Inhibition of Candida albicans Growth by Murine Peritoneal Neutrophils and Augmentation of the Inhibitory Activity by Bacterial Lipopolysaccharide and Cytokines

Abstract

Anti‐Candida activity of murine neutrophils and its regulation by immunomodulators were studied in vitro. Murine neutrophils which were prepared from peritoneal‐exudated cells inhibited the growth of Candida albicans at an effector: target (E/T) ratio of 30/1 or above. This anti‐Candida activity of neutrophils was augmented by lipopolysaccharide from Escherichia coli, murine tumor necrosis factor (TNF), murine interferon‐γ (IFN‐γ) and murine granulocyte macrophage colony‐stimulating factor (GM‐CSF) but not by granulocyte colony‐stimulating factor (G‐CSF) added to the incubation medium. Greater extent of augmentation was obtained when TNF plus GM‐CSF or INF‐γ plus GM‐CSF were used in combination. These results indicate that anti‐Candida activity of murine neutrophils is regulated similarly to that of the human neutrophils reported previously. Therefore murine peritoneal neutrophils can be used as a favorable substitute for human neutrophils in studies on protective machinery against C. albicans infection.

CD8– dendritic cells preferentially cross‐present Saccharomyces cerevisiae antigens

“…A few studies have reported direct killing of yeasts by CD8 + T cells [58,59]. Alternatively IFN-c or TNF-a, produced by activated CD8 + T cells, could enhance anti-fungal activity of macrophages and neutrophils [60], since IFN-c is used as therapy for fungal infections [61].…”

“…A few studies have reported direct killing of yeasts by CD8 + T cells [58,59]. Alternatively IFN-c or TNF-a, produced by activated CD8 + T cells, could enhance anti-fungal activity of macrophages and neutrophils [60], since IFN-c is used as therapy for fungal infections [61].Previously, we have shown that CD8 -DC can crosspresent immune complexes [36]. In CD8 -DC this process is dependent on the signaling FcR c-chain, whereas cross-presentation by CD8 + DC does not require the FcR c-chain.…”

Effect of orally administered bovine lactoferrin on the immune response in the oral candidiasis murine model

“…It is well known that TNF-AE and IFN-ª stimulate macrophages and neutrophils, which kill C. albicans directly (Tansho et al, 1994). Farah et al (2002) demonstrated that TNF-AE in oral tissue was an important mediator in the recovery from oropharyngeal candidiasis in mice.…”

A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

“…This assay in microwell plates made possible accurate and objective measurement of Candida growth under the influence of a limited number of effector cells. In our experience with this assay technique, we noticed that 3H-glucose incorporation seemed to be correlated with the degree of hyphal growth of C. albicans which could be observed microscopically (5). We therefore wanted to develop a new nonradiolabel assay which rapidly and conventionally measured the effector cell inhibition of Candida growth.…”