A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of <i>Candida albicans</i>

Abe, Shigeru; Satoh, Tamae; Tokuda, Yoshiko; Tansho, Shigeru; Yamaguchi, Hideyo

doi:10.1111/j.1348-0421.1994.tb01795.x

Cited by 49 publications

(39 citation statements)

References 6 publications

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“…C. albicans TIMM1768 which had been stored at Ϫ80 C in the Research Center for Mycology at Teikyo University was used in this study (1). After thawing, 10 6 cells were inoculated to 10 ml of Sabouraud glucose liquid medium supplemented with 0.5% yeast extract.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Effector Cells with Anti‐Candida Activity Obtained from Murine Bone Marrow Cells Cultured in the Presence of rhG‐CSF: Comparison between Normal and CY‐Treated Mice

Morofuji

Abe

Okinaga

2005

Microbiology and Immunology

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Bone marrow cells (BMC) obtained from normal and cyclophosphamide (CY)-treated mice were cultured in the presence of recombinant human granulocyte-colony stimulating factor (rhG-CSF) and their effector cell activities inhibiting growth of C. albicans were examined. When BMC from CY-treated mice were preincubated with 0.05 ng/ml of rhG-CSF, effector cells with enhanced anti-C. albicans activity were recovered in the adherent cell population, whereas anti-C. albicans activity of BMC from normal mice was found in the non-adherent cell population. During culture without the presence of rhG-CSF, nonadherent BMC, seemingly granulocytes, from normal mice showed apoptotic change, but addition of rhG-CSF clearly inhibited this change. On the other hand, when BMC from CY-treated mice were cultured with rhG-CSF, adherent cells as the main effector had the appearance of monocytes. These differences between the effectors with anti-C. albicans activity obtained from normal and CY-treated mice are discussed.

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Section: Methodsmentioning

confidence: 99%

Characterization of Effector Cells with Anti‐Candida Activity Obtained from Murine Bone Marrow Cells Cultured in the Presence of rhG‐CSF: Comparison between Normal and CY‐Treated Mice

Morofuji

Abe

Okinaga

2005

Microbiology and Immunology

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“…In Vitro Activity of Geranium Oil and Geraniol against Candida Mycelial Growth The mycelial growth of C. albicans was measured based on the method of Abe et al 16) Geranium oil and geraniol were dissolved in dimethyl sulfoxide (DMSO) in 100 mg/ml solution. Geranium oil was further diluted using RPMI1640 containing 2.5% FCS to 50, 100, 200, 400 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

Protective Activity of Geranium Oil and Its Component, Geraniol, in Combination with Vaginal Washing against Vaginal Candidiasis in Mice

Maruyama

Takizawa

Ishibashi

et al. 2008

Biological & Pharmaceutical Bulletin

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In order to evaluate an effective administration method of essential oils for vaginal candidiasis, efficacy of vaginal application of essential oils against murine experimental candidiasis was investigated. The effect on vaginal inflammation and Candida growth form was also studied. Vaginal candidiasis was established by intravaginal infection of C. albicans to estradiol-treated mice. These mice intravaginally received essential oils such as geranium and tea tree singly or in combination with vaginal washing. Vaginal administration of clotrimazole significantly decreased the number of viable C. albicans cells in the vaginal cavity by itself. In contrast, these essential oils did not lower the cell number. When application of geranium oil or geraniol was combined with vaginal washing, the cell number was decreased significantly. The myeloperoxidase activity assay exhibited the possibility that essential oils worked not only to reduce the viable cell number of C. albicans, but also to improve vaginal inflammation. The smear of vaginal washing suspension suggested that more yeast-form cells appeared in vaginal smears of these oil-treated mice than in control mice. In vitro study showed that a very low concentration (25 m mg/ml) of geranium oil and geraniol inhibited mycelial growth, but not yeast growth. Based on these findings, it is estimated that vaginal application of geranium oil or its main component, geraniol, suppressed Candida cell growth in the vagina and its local inflammation when combined with vaginal washing.

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“…We focused our attention on the examination of the effects of resveratrol on C. albicans growth, particularly the effects on morphological transition from single yeast cells to hyphal filaments under various hyphae-inducing conditions. To determine the effects of resveratrol on mycelial growth of C. albicans, a crystal violet (CV, Wako Pure Chemical Industries, Osaka, Japan)-staining assay developed by Abe et al [1,2] was performed. (Fig.…”

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Resveratrol Impaired the Morphological Transition of Candida albicans Under Various Hyphae-Inducing Conditions

Okamoto-Shibayama

Satō²,

Azuma³

2010

J. Microbiol. Biotechnol.

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The ability of the human fungal pathogen Candida albicans to undergo the morphological transition from a single yeast form to pseudohyphal and hyphal forms in response to various conditions is known to be an important for its virulence. Many studies have shown the pharmacological effects of resveratrol, a phytoalexin polyphenolic compound. In this study, we investigated the antifungal activity of resveratrol against C. albicans. Both yeast-form and mycelial growth of C. albicans were inhibited by resveratrol. In addition, normal filamentation of C. albicans was affected and yeast-to-hypha transition under serum-, pH-, and nutrient-induced hyphal growth conditions was impaired by resveratrol.Key words Candida albicans; resveratrol (trans-3,4',-5-trihydroxystilebene); dimorphism 3 Candida albicans, a major fungal pathogen causing mucosal and systemic infections in immuno-compromised hosts [5,16], is dimorphic and grows as yeast as well as filamentous modes in host organisms and in vitro [19]. A key property of this fungus is its ability to switch from the yeast to the hyphal form in the host and this has been implicated in the process of pathogenesis since mutants defective in hyphal growth are known to be less virulent in systemic infections [15]. This transition is known to occur in response to a variety of environmental conditions, such as the presence of serum, body temperature (37°C), neutral pH, and growth with a poor carbon source [19].Resveratrol (trans-3,4',-5-trihydroxystilebene) is a phytoalexin polyphenolic compound produced by the innate host defense systems of plants [3,9]. Although many studies have shown various pharmacological effects of resveratrol, for instance, antiviral properties, protective effects against inflammation, enhancement of stress resistance, and lifespan extension [3,4,7], little is known about their effects on fungi. The antifungal activity of resveratrol was first demonstrated by Jung et al. [10,11]. However Weber et al. [21] recently mentioned that the potential candidacidal activity of resveratrol was not confirmed. The effective concentration of resveratrol against C. albicans observed by other groups varies considerably [6,13]. This suggests the difficulties still remain in the treatment with such biological agent. Therefore, there are great interests in the effects of resveratrol on C.albicans. The present study was designed to gain a better understanding the antifungal activity of resveratrol. We focused our attention on the examination of the effects of resveratrol on C. albicans growth, particularly the effects on morphological transition from single yeast cells to hyphal filaments under various hyphae-inducing conditions. (Fig. 2).For experiments involving hyphal growth, C. albicans yeast cell suspensions were spread on hyphae-inducing solid media without or with resveratrol and the colonies were photographed after 6-day incubation at 37°C. Resveratrol impairs the yeast-to-hyphae transition and induces colony morphological changes of C. albicans (Fig. 3). Normally, C....

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A Rapid Colorimetric Assay for Determination of Leukocyte‐Mediated Inhibition of Mycelial Growth of Candida albicans

Cited by 49 publications

References 6 publications

Characterization of Effector Cells with Anti‐Candida Activity Obtained from Murine Bone Marrow Cells Cultured in the Presence of rhG‐CSF: Comparison between Normal and CY‐Treated Mice

Characterization of Effector Cells with Anti‐Candida Activity Obtained from Murine Bone Marrow Cells Cultured in the Presence of rhG‐CSF: Comparison between Normal and CY‐Treated Mice

Protective Activity of Geranium Oil and Its Component, Geraniol, in Combination with Vaginal Washing against Vaginal Candidiasis in Mice

Resveratrol Impaired the Morphological Transition of Candida albicans Under Various Hyphae-Inducing Conditions

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