Inhibition of <i>Candida albicans</i>
 biofilm and hyphae formation by biocompatible oligomers

Lee, J.-H.; Kim, Y.-G.

doi:10.1111/lam.13016

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…56 In view of this, many studies have investigated the potential of yeast pseudohyphae inhibition as new therapies for treatment of mycoses in general. 38,56,57 In addition to the inhibition of pseudohyphae formation in C albicans, it was also demonstrated for this yeast that the cell surface appeared to be altered and irregular by the compound 2j ( Figure 3J) and anidulafungin ( Figure 3F Figure 3J) suggesting that the normal blastoconidium division process may have been impaired resulting in single cells with multiple attempts cycle defects, which has already been verified for Candida cells exposed to fluconazole. 58 Structural changes in filamentous fungi after in vitro treatment with compound 2j were also evaluated by SEM.…”

Section: Scanning Electron Microscopy Imagessupporting

confidence: 60%

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Discovery of a novel and selective fungicide that targets fungal cell wall to treat dermatomycoses: 1,3‐bis(3,4‐dichlorophenoxy)propan‐2‐aminium chloride

Lana

Lavorato

Giuliani

et al. 2019

Mycoses

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Summary Background Fungal infections are highly prevalent and are responsible for high rates of morbidity and mortality. In this context, the search for new treatment alternatives is very relevant. Objectives Analyse chemical compounds for antifungal potential against dermatomycosis fungi. Methods The antifungal activity of 121 compounds, intermediates or derivatives of 1,3‐bis(aryloxy)propane substituted at C‐2 (111 compounds) and isothiouronium derivatives (10 compounds) was investigated through susceptibility tests, mechanism of action, toxicity and hydrogel incorporation. Results The compound 1,3‐bis(3,4‐dichlorophenoxy)propan‐2‐aminium chloride (2j) was the most active fungicide against dermatophytes and Candida spp., at very low concentrations (0.39‐3.12 µg/mL), including action on resistant and multidrug‐resistant clinical strains. Compound 2j has presented a promising toxicity profile, showing selectivity index >10, relative to human lymphocytes. The compound was classified as non‐irritant by the HET‐CAM test and did not cause histopathological alterations in pig ear skin, thus presenting an excellent perspective for topical application. 2j targets the fungal cell wall, which was confirmed by scanning electron microscopy, which also indicated the additional ability of 2j to inhibit the Candida albicans pseudohyphae formation and biofilm of Microsporum canis. Compound 2j was incorporated in a hydrogel with bioadhesive potential. The results of the human skin permeation showed that 2j remained significantly in the epidermis, ideally for the dermatomycosis treatment. Conclusions Therefore, the compound 2j demonstrated the potential for antifungal drug development, with a action mechanism elucidated and already applied in a semisolid formulation as a new therapeutic option for fungal skin infections.

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Section: Scanning Electron Microscopy Imagessupporting

confidence: 60%

“…The ability of filamentous forms formation is an important factor for pathogenicity at dermatomycosis and invasive fungal infections . In view of this, many studies have investigated the potential of yeast pseudohyphae inhibition as new therapies for treatment of mycoses in general …”

Section: Resultsmentioning

confidence: 99%

Discovery of a novel and selective fungicide that targets fungal cell wall to treat dermatomycoses: 1,3‐bis(3,4‐dichlorophenoxy)propan‐2‐aminium chloride

Lana

Lavorato

Giuliani

et al. 2019

Mycoses

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“…Candida albicans is a fungal pathogen that easily colonizes host tissues and implant devices forming mature biofilms directly involved in pathogenesis and drug resistance [18]. On the other hand, the pathogenicity of this opportunistic human fungal pathogen depends on the competence of host immune response that if diminishes, C. albicans behaves like a true pathogen.…”

Section: Introductionmentioning

confidence: 99%

Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candidaalbicans interface

Diez-Orejas

Casarrubios

Feito

et al. 2021

International Immunopharmacology

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The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects of the drug but, the uptake of these nanocarriers could induce adverse effects on cells and tissues after their incorporation. Macrophages play a central role in host defense and are responsible for in vivo nanoparticle trafficking. Assessment of their defense capacity against pathogenic micro-organisms after nanoparticle uptake, is necessary to prevent infections associated with nanoparticle therapies. In this study, the effects of hollow mesoporous SiO2-CaO nanospheres labeled with fluorescein isothiocyanate (FITC-NanoMBGs) on the function of peritoneal macrophages was assessed by measuring their ability to phagocytize Candida albicans expressing a red fluorescent protein. Two macrophage/fungus ratios (MOI 1 and MOI 5) were used and two experimental strategies were carried out: a) pretreatment of macrophages with FITC-NanoMBGs and subsequent fungal infection; b) competition assays after simultaneous addition of fungus and nanospheres. Macrophage pro-inflammatory phenotype markers (CD80

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“…Currently, there have been many advanced technologies used to combat fungal infections due to C. albicans biofilm formation, including antifungal chemotherapies , and surface modifications by coating polymers , or using plasma techniques. , …”

Section: Introductionmentioning

confidence: 99%

“…Biofilm formation by C. albicans occurs in four stages: (1) the attachment and propagation of round yeast cells to the surface; (2) the initial attachment, in which cells grow in a dense community to form a basal layer for anchoring, after which a transformation to the biofilm-forming phenotype occurs (cells are no longer round and form pseudohyphae and hyphae); (3) the maturation phase, where the biofilm-type cells are enclosed in the extracellular polymeric substances (EPS); and (4) the dispersal phase, where the cells are dispersed from the mature biofilm. 9,10 Currently, there have been many advanced technologies used to combat fungal infections due to C. albicans biofilm formation, including antifungal chemotherapies 11,12 and surface modifications by coating polymers 13,14 or using plasma techniques. 15,16 While many studies have been focused on comprehending the Candida molecular processes involved in biofilm formation in order to prevent biofilm formation, 17,18 there has been less attention on understanding whether the modifications of surface topography can influence C. albicans biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale Surface Roughness Influences Candida albicans Biofilm Formation

Nguyên

Aburto‐Medina

et al. 2020

ACS Appl. Bio Mater.

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The microbial contamination of surfaces presents a significant challenge due to the adverse effects associated with biofilm formation, particularly on implantable devices. Here, the attachment and biofilm formation of the opportunistic human pathogen, Candida albicans ATCC 10231, were studied on surfaces with decreasing magnitudes of nanoscale roughness. The nanoscale surface roughness of nonpolished titanium, polished titanium, and glass was characterized according to average surface roughness, skewness, and kurtosis. Nonpolished titanium, polished titanium, and glass possessed average surface roughness (S a) values of 350, 20, and 2.5 nm; skewness (S skw) values of 1.0, 4.0, and 1.0; and (S kur) values of 3.5, 16, and 4, respectively. These unique characteristics of the surface nanoarchitecture were found to play a key role in limiting C. albicans attachment and modulating the functional phenotypic changes associated with biofilm formation. Our results suggest that surfaces with a specific combination of surface topographical parameters could prevent the attachment and biofilm formation of C. albicans. After 7 days, the density of attached C. albicans cells was recorded to be 230, 70, and 220 cells mm–2 on nonpolished titanium, polished titanium, and glass surfaces, respectively. Despite achieving a very low attachment density, C. albicanscells were only observed to produce hyphae associated with biofilm formation on nonpolished titanium surfaces, possessing the highest degree of surface roughness (S a = 350 nm). This study provides a more comprehensive picture of the impact of surface architectures on C. albicans attachment, which is beneficial for the design of antifungal surfaces.

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Inhibition of Candida albicans biofilm and hyphae formation by biocompatible oligomers

Cited by 12 publications

References 19 publications

Discovery of a novel and selective fungicide that targets fungal cell wall to treat dermatomycoses: 1,3‐bis(3,4‐dichlorophenoxy)propan‐2‐aminium chloride

Discovery of a novel and selective fungicide that targets fungal cell wall to treat dermatomycoses: 1,3‐bis(3,4‐dichlorophenoxy)propan‐2‐aminium chloride

Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candidaalbicans interface

Nanoscale Surface Roughness Influences Candida albicans Biofilm Formation

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