Inhibition of human pancreatic cancer growth in nude mice by boron neutron capture therapy

Abstract

Histopathologically, hyalinization and necrosis were found in '0B-treated tumours, while tumour tissue injected with saline or saline-containing immunoliposomes showed neither destruction nor necrosis. These results suggest that intratumoral injection of boronated immunoliposomes can increase the retention of '0B atoms by tumour cells, causing tumour growth suppression in vivo upon thermal neutron irradiation. Boron neutron capture therapy (BNCT) with intratumoral injection of immunoliposomes is able to destroy malignant cells in the marginal portion between normal tissues and cancer tissues from the side of 4He generation.

Antitumour effect of polyoxomolybdates: induction of apoptotic cell death and autophagy in in vitro and in vivo models

“…We plan to investigate the induction mechanism of tumour cell death in detail, especially how PM-17 turns the cell death programme on, and continue research for in vivo models of antitumour therapy and the development of novel drug-delivery systems using PM-17 (Yanagie et al, 1997(Yanagie et al, , 2004. …”

Boron delivery agents for neutron capture therapy of cancer

“…Targeting moieties such as MoAbs [ 92 ], antibodies directed against carcinoembryonic antigens (CEA) [ 100 ], transferrin [ 101 ], and EGFR [ 102 ] also have been introduced on the surface of liposomes to specifically target tumor cells. These immunoliposomes could deliver low molecular weight hydrophobic agents such as BSH that have been incorporated into their lipid bilayers [ 102 , 103 ], and liposomes can transport large numbers of boron-containing molecules intracellularly, resulting in high tumor boron uptake [ 104 ].…”

Biopharmaceutics of boronated radiosensitizers: Liposomal formulation of MnBOPP (manganese chelate of 2,4‐(α,β‐dihydroxyethyl) deuterioporphyrin IX) and comparative toxicity in mice

“…17,18,20 Because of the beneficial effects of encapsulation on toxicity and selectivity of deposition, liposomes hold considerable promise for the delivery of boronated compounds in NCT. [21][22][23][24] With the reduction in toxicity observed here, dose escalation and possibly increased tumor uptake of MnBOPP may be achievable via liposome encapsulation. However, liposomal formulation of lipophilic compounds involves optimization of the large number of parameters that affect stability; 25-27 these parameters include the preferred membrane physical state, determined by the phase transition temperature (T m ) of the predominant lipid constituent, the lipid acyl chain composition, and the CHOL content, which both modifies T m and has the effect of stabilizing liposomes in vivo.…”