Inhibition of human ovarian cancer cell lines by Devil's club Oplopanax horridus

Tai, Joseph; Cheung, Susan; Chan, Edwin; Hasman, David

doi:10.1016/j.jep.2009.10.010

Cited by 16 publications

(12 citation statements)

References 17 publications

(19 reference statements)

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“…The combinational cytotoxic effect of subamolide A (1, 5 and 10 M) with CDDP (1 and 3 M) or Gem (2 and 8 nM) was determined in NTUB1 cells with MTT assay for 24, 48 and 72 h in triplicates, respectively. The proportions of surviving cells were determined and expressed as the ratio to the un-treated control Tai et al, 2010). The combinational effects of subamolide A with CDDP and Gem were analyzed by median-effect analysis and the computer software Calcusyn (version 1.1.1, 1996, Biosoft Inc., Cambridge, UK).…”

Section: Analysis Of Cytotoxicity With Mtt Assay and Observation Of Cmentioning

confidence: 99%

Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1

Liu

Chen

Huang

et al. 2011

Journal of Ethnopharmacology

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Section: Analysis Of Cytotoxicity With Mtt Assay and Observation Of Cmentioning

confidence: 99%

Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1

Liu

Chen

Huang

et al. 2011

Journal of Ethnopharmacology

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“…Natural products have promising results as potential treatment for cancer in general and ALL in particular (Jadhav et al, 2007;Tai et al, 2010;Abdel-Massih et al, 2010;Davicino et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Bassil¹,

Abdel-Massih²,

El-Chami³

et al. 2012

JPS

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We tested the effect of ethanol extracts from Ruscus aculeatus (Asparagaceae) and Pleurotus ostreatus (Agaricales) on the survival and proliferation of the Jurkat cell line, a model of human acute T-cell lymphocytic leukemia. R. aculeatus extracts were toxic to both Jurkat cells and human primary lymphocytes. P. ostreatus extracts were significantly more toxic to Jurkat cells than primary lymphocytes, as seen by LDH release and MTT assays. Annexin-V detection as well as Bax, Bcl-2, Smac/Diablo, and Caspase-8 expression were largely unchanged by P. ostreatus extracts. Cell cycle analysis and CDK-4 expression revealed stop of Jurkat cell proliferation. Interestingly, both extracts cause a strong, dose-dependent decrease in p53 levels in Jurkat cells, consistent with toxicity and cell death occurring via an unknown, non-apoptotic mechanism.

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“…Recently, the O. horridus extract was found to have potential anticancer activity on a number of cancer cell lines in vitro (Tai et al, 2009; Tai et al, 2006; Wang et al, 2009). Owing to unavailability of chemical entities, however, it is unclear which are bioactive compounds possessing inhibitory effects on cancer cells in this plant.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic constituents and their potential anticancer activities from Devil's Club (Oplopanax horridus Miq.)

Sun

et al. 2010

Journal of Ethnopharmacology

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Ethnopharmacological relevance-Devil's Club Oplopanax horridus (Sm.) Miq. is a widely used folk medicine in the Pacific Northwest such as Alaska and British Columbia for treating a variety of ailments including arthritis, cold, fever, infections, diabetes and cancer.Aim of the study-To investigate hydrophobic constituents and their potential anticancer activity from Devil's Club O. horridus. Materials and methods-The root bark of O. horridus (Sm.) Miq was isolated by chromatographic techniques. Structures of isolated compounds were identified by spectroscopic methods and comparison with published data. The anti-proliferation of isolated hydrophobic constituents in human breast cancer MCF-7 cells, human colon cancer SW-480 and HCT-116 cells were tested. The potential mechanism of anti-proliferation was also investigated using cell cycle and apoptosis assays.Results and discussion-Six compounds were isolated and structurally identified as 9,17-octadecadiene-12,14-diyne-1,11,16-triol, 1-acetate (1), oplopandiol acetate (2), falcarindiol (3), oplopandiol (4), trans-nerolidol (5) and t-cadinol (6). These compounds showed potential anticancer activities on human breast cancer and colon cancer cells, of which compound 3 possesses the strongest activity. Further cell cycle and apoptosis test by flow cytometry showed the polyacetylenes 1-4 induced HCT-116 cell arresting in G2/M phase and inhibited proliferation by the induction of apoptosis at both earlier and later stage.Conclusion-These results provide promising baseline information for the potential use of O. horridus as well as some of the isolated compounds in the treatment of cancer.

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Inhibition of human ovarian cancer cell lines by Devil's club Oplopanax horridus

Cited by 16 publications

References 17 publications

Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1

Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1

Pleurotus ostreatus and Ruscus aculeatus Extracts Cause Non-Apoptotic Jurkat Cell Death

Hydrophobic constituents and their potential anticancer activities from Devil's Club (Oplopanax horridus Miq.)

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