Inhibition of human <i>in vitro</i> osteoclastogenesis by <i><scp>E</scp>quisetum arvense</i>

Costa‐Rodrigues, João; Carmo, S.; Silva, J. C.; Fernandes, Maria Helena

doi:10.1111/j.1365-2184.2012.00848.x

Cited by 27 publications

(23 citation statements)

References 50 publications

(82 reference statements)

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“…Co-culture of monocytes with osteoblasts is a well-known method of generating osteoclasts, utilising the RANKL supplied by the osteoblastic cells to induce differentiation. Our data that inhibition of osteoclast differentiation by FG-4592 was greater under co-culture with osteoblasts is similar to that previously described with reagents such as magnesium extract [41], triptolide [42] and Equisetum arvense [43]. It is possible that this is due to changes in the RANKL:OPG ratio, although our data regarding effects on OPG:RANKL expression was variable and inconclusive (data not shown).…”

Section: Discussionsupporting

confidence: 87%

Osteoblast-osteoclast co-culture amplifies inhibitory effects of FG-4592 on osteoclast formation and reduces bone resorption activity

Hulley

Papadimitriou-Olivgeri

Knowles

2019

Preprint

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The link between bone and blood vessels is regulated by hypoxia and the hypoxia-inducible transcription factor, HIF, which drives both osteogenesis and angiogenesis. The recent clinical approval of PHD enzyme inhibitors, which stabilise HIF protein, introduces the potential for a new clinical strategy to treat osteolytic conditions such as osteoporosis, osteonecrosis and skeletal fracture and non-union. However, bone-resorbing osteoclasts also play a central role in bone remodelling and pathological osteolysis and HIF promotes osteoclast activation and bone loss in vitro. It is therefore likely that the final outcome of PHD enzyme inhibition in vivo would be mediated by a balance between increased bone formation and increased bone resorption. It is essential that we improve our understanding of the effects of HIF on osteoclast formation and function, and consider the potential contribution of inhibitory interactions with other musculoskeletal cells.The PHD enzyme inhibitor FG-4592 stabilised HIF protein and stimulated osteoclast-mediated bone resorption, but inhibited differentiation of human CD14+ monocytes into osteoclasts. Formation of osteoclasts in a more physiologically relevant 3D collagen gel did not affect the sensitivity of osteoclastogenesis to FG-4592, but increased sensitivity to reduced concentrations of RANKL. Coculture with osteoblasts amplified inhibition of osteoclastogenesis by FG-4592, whether the osteoblasts were proliferating, differentiating or in the presence of exogenous M-CSF and RANKL. Osteoblast co-culture dampened the ability of high concentrations of FG-4592 to increase bone resorption.This data provides support for the therapeutic use of PHD enzyme inhibitors to improve bone formation and/or reduce bone loss for treatment of osteolytic pathologies, and indicates that FG-4592 might also act to inhibit the formation and activity of the osteoclasts that drive osteolysis.

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Section: Discussionsupporting

confidence: 87%

Osteoblast-osteoclast co-culture amplifies inhibitory effects of FG-4592 on osteoclast formation and reduces bone resorption activity

Hulley

Papadimitriou-Olivgeri

Knowles

2019

Preprint

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“…Therefore, using active ingredients of medicinal plants in bone regeneration studies have a promising potential as alternative options. There are many pieces of evidence in in vitro and in vivo studies that show some herb species have a positive effect on bone metabolism . As a typical example Suganya et al .…”

Section: Introductionmentioning

confidence: 99%

Physical, morphological, and biological studies on PLA/nHA composite nanofibrous webs containing Equisetum arvense herbal extract for bone tissue engineering

Khakestani

Jafari

Zahedi

et al. 2017

J of Applied Polymer Sci

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A series of herbal extract incorporated into poly(lactic acid) (PLA) composite nanofibrous scaffolds were successfully prepared by using electrospinning technique. Equisetum arvense extract (EE) and nanohydroxyapatite (nHA) in different quantities were loaded into PLA solution to fabricate composite nanofibrous webs under various electrospinning conditions. Uniform nanofibers were obtained with an average diameter of 157 6 47 nm in the case of those containing the herbal extract. Characterization of the webs was carried out by means of Fourier transform infrared (FTIR) spectroscopy, field emission-scanning electron microscopy (FESEM), energydispersive X-ray spectroscopy (EDX), and differential scanning calorimetry (DSC) techniques. Mechanical properties, porosity, and contact angle of the prepared webs were also determined. Releasing behavior was investigated in phosphate buffer solution (pH 7.2) medium. Moreover, cell studies and osteogenic capacity were assessed in vitro using human adipose tissue-derived mesenchymal stem cell (AT-MSC). Evaluations of cell attachment, spreading, and proliferation of AT-MSC were done by SEM observation and thiazolyl blue (MTT) assay. Osteogenic differentiation capability of AT-MSC on the nanofibrous webs was analyzed by alkaline phosphatase activity and calcium content assay. It was found that with the addition of nHA and EE to PLA nanofibrous webs, their surface hydrophobicity was reduced while the tensile strength and Young's modulus were increased satisfactorily. Regarding the samples containing EE and nHA, cellular adhesion was observed with flattened normal morphology. Osteogenic differentiation of AT-MSC on PLA/nHA/EE webs showed the highest mineralization capacity after 3 weeks which, was about 1.8 and 3 times higher than that of PLA/nHA and tissue culture polystyrene as control, respectively.

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“…The extract reduced human osteoclast development and function, both in osteoclast precursor cell cultures and in cocultures of osteoclastic and osteoblastic cells. In studying of the effect of hydromethanolic extract on behavior of human bone marrow cells for osteoblastic modulation in vitro, the extract promoted osteoblastic response while preventing risk of infection at the biomaterial/ bone interface by local delivery system [105][106].…”

Section: Effect On Bonesmentioning

confidence: 99%

The pharmacology of Equisetum arvense- A review

Al-Snafi¹

2017

IOSRPHR

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Abstract:-The previous studies revealed that Equisetum arvense contained alkaloids, carbohydrate, proteins and amino acids, phytosterols, saponins, sterols, ascorbic acid, silicic acid, phenol , tannin, flavonoids, triterpenoids, volatile oils and many other biological active constituents. The pharmacological studies showed that it possessed antioxidant, anticancer, antimicrobial, smooth muscle relaxant effects of the vessels and ileum, anticonvulsant, sedative, anti-anxiety, dermatological immunological, antinociceptive, anti-inflammatory, antidiabetic, diuretic, inhibition of platelet aggregation, promotion of osteoblastic response, anti-leishmanial, and many other effects. The current review highlights the chemical constituents and pharmacological effects of Equisetum arvense.

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Inhibition of human in vitro osteoclastogenesis by Equisetum arvense

Cited by 27 publications

References 50 publications

Osteoblast-osteoclast co-culture amplifies inhibitory effects of FG-4592 on osteoclast formation and reduces bone resorption activity

Osteoblast-osteoclast co-culture amplifies inhibitory effects of FG-4592 on osteoclast formation and reduces bone resorption activity

Physical, morphological, and biological studies on PLA/nHA composite nanofibrous webs containing Equisetum arvense herbal extract for bone tissue engineering

The pharmacology of Equisetum arvense- A review

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