Inhibition of human glioma cell proliferation by altered Bax/Bcl-2-p53 expression and apoptosis induction by <i>Rhaponticum carthamoides</i> extracts from transformed and normal roots

Skała, Ewa; Sitarek, Przemysław; Toma, Monika; Szemraj, Janusz; Radek, Maciej; Nieborowska-Skorska, Malgorzata; Skórski, Tomasz; Wysokińska, Halina; Śliwiński, Tomasz

doi:10.1111/jphp.12619

Cited by 27 publications

(33 citation statements)

References 28 publications

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“…Thus, the increasing Bax/Bcl-2 ratio results in apoptosis in human glioblastoma cells while the decreasing Bax/Bcl-2 ratio represses cell apoptosis and contributes to the resistance of glioblastoma cells to chemotherapeutic agent (28). It is reported that the upregulation of Bax/Bcl-2 resulted in the loss of mitochondrial membrane potential (MMP), inducing cell apoptosis via the intrinsic pathway in glioma cells (29). Since the ratio of Bax/Bcl-2 is regulated by ERK1/2 signaling pathway (30), we hypothesized that SFN-Cys might induce intrinsic apoptosis by upregulating Bax/Bcl-2 ratio mediated via the activated ERK1/2.…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane-cysteine induces apoptosis by sustained activation of ERK1/2 and caspase 3 in human glioblastoma U373MG and U87MG cells

Zhou

Yang

et al. 2017

Oncology Reports

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We previously demonstrated that sulforaphane (SFN) inhibited invasion via sustained activation of ERK1/2 in human glioblastoma cells. However, sulforaphane-cysteine (SFN-Cys), an analog of SFN, enriched in plasma with longer half-life, had more potentiality to induce apoptosis. Here we investigated the molecular mechanisms of SFN-Cys-induced apoptosis in human glioblastoma U373MG and U87MG cells. Cell viability assay showed that SFN-Cys inhibited cell viability in a dose-dependent manner. Cell morphology observation also showed SFN-Cys increased the phenotype of cell death in a dose-dependent manner. Furthermore, flow cytometry assay showed that SFN-Cys induced apoptosis significantly in a dose-dependent manner in both cell lines. Furthermore, western blot analysis demonstrated that SFN-Cys induced activation of ERK1/2 in a sustained manner and the activation contributed to upregulation of Bax/Bcl-2 ratio and cleaved caspase 3, and these results can be reversed by the ERK1/2 blocker PD98059. Our results showed that SFN-Cys induced cell apoptosis via sustained activation of ERK1/2 and the ERK1/2 mediated signaling pathways such as activation of caspase 3 and apoptosis-related proteins, thus indicating that SFN-Cys might be a more promising therapeutic agent versus SFN to resist glioblastoma cells, especially in Taxol-resistant cancer cells.

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Section: Introductionmentioning

confidence: 99%

Sulforaphane-cysteine induces apoptosis by sustained activation of ERK1/2 and caspase 3 in human glioblastoma U373MG and U87MG cells

Zhou

Yang

et al. 2017

Oncology Reports

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“…Iljin (Asteraceae) has been used for centuries in traditional Siberian medicine in the case of overstrain and weakness after illness [8]. The plant has demonstrated several biological activities, including adaptogenic, antioxidant, anticancer, and antimicrobial effects [8–10]. The raw material of R. carthamoides comprises roots and rhizomes of three- to four-year-old plants which are used in various dietary supplements or nutraceutical preparations.…”

Section: Introductionmentioning

confidence: 99%

The Essential Oils of Rhaponticum carthamoides Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities

Skała

Rijo

García

et al. 2016

Oxidative Medicine and Cellular Longevity

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The essential oils were isolated by hydrodistillation from the hairy roots (HR) and roots of soil-grown plants (SGR) of Rhaponticum carthamoides and were analyzed by GC-MS method. In the both essential oils 62 compounds were identified. The root essential oils showed the differences in the qualitative and quantitative composition. The sesquiterpene hydrocarbons (55–62%) dominated in both essential oils. The major compounds of HR essential oil were cyperene, 13-norcypera-1(5),11(12)-diene, and cadalene while aplotaxene, nardosina-1(10),11-diene, and dauca-4(11),8-diene dominated in SGR essential oil. Both essential oils showed antibacterial activity especially against Enterococcus faecalis (ATCC 29212) and Pseudomonas aeruginosa (ATCC 27853) (MIC value = 125 µg/mL). HR and SGR essential oils also decreased the expression of IL-1β, IL-6, and TNF-α and the ROS level in LPS-treatment astrocytes. This is the first report to describe the chemical composition of R. carthamoides essential oil from hairy roots, its protective effect against LPS-induced inflammation and ROS production in astrocytes, and its antimicrobial potential. The results show that R. carthamoides hairy roots may be a valuable source of the essential oil and may be an alternative to the roots of soil-grown plants.

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“…However, a pervious study shows that overexpression of Bax accelerates apoptosis induced by cytokine deprivation in cell lines (31). Ska"a et al (32) indicated that the high expression of pro-apoptotic Bax protein and low expression of anti-apoptotic Bcl-2 protein are beneficial to inhibit cell proliferation and promote apoptosis. More importantly, Bcl-2 protein also activates the downstream caspase cascade by regulating mitochondrial extracorporeal membrane permeabilization to perform apoptosis (33).…”

Section: Discussionmentioning

confidence: 99%

Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis

Zhang

Wang

et al. 2020

Braz J Med Biol Res

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This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics +empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, proliferation, and apoptosis in HSECs. Moreover, the levels of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), Bcl-2, p-P13K, and p-AKT in HAECs were detected by western blot. MiR-106b was down-regulated in ox-LDL-induced HAECs. PTEN was the target gene of miR-106b-5p. Overexpression of PTEN inhibited the anti-apoptotic effect of miR-106b. Compared with the control group, the proportion and number of HAECs apoptosis and Bax, caspase-3, and caspase-9 expression in ox-LDL and miR-106b mimics+PTEN+ox-LDL groups were significantly increased (all Po0.05). Moreover, the activity of HAECs and Bcl-2 were decreased significantly (all Po0.05). Overexpression of miR-106b in ox-LDLinduced AS inhibited endothelial cell apoptosis. Furthermore, miR-106b might activate the PI3K/AKT pathway by downregulating the expression of PTEN in ox-LDL-induced HAECs.

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Inhibition of human glioma cell proliferation by altered Bax/Bcl-2-p53 expression and apoptosis induction by Rhaponticum carthamoides extracts from transformed and normal roots

Abstract: The results of this study may offer a new insight into the potential anticancer activity of R. carthamoides root extracts.

Cited by 27 publications

References 28 publications

Sulforaphane-cysteine induces apoptosis by sustained activation of ERK1/2 and caspase 3 in human glioblastoma U373MG and U87MG cells

Sulforaphane-cysteine induces apoptosis by sustained activation of ERK1/2 and caspase 3 in human glioblastoma U373MG and U87MG cells

The Essential Oils of Rhaponticum carthamoides Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities

Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis

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