Inhibition of human breast cancer Matrigel invasion by Streptolysin O activation of the EGF receptor ErbB1

Hall, Emily H.; Gurel, Volkan; Dahĺberg, Albert E.; McMichael, John; Brautigan, David L.

doi:10.1016/j.cellsig.2011.07.007

Cited by 9 publications

(6 citation statements)

References 15 publications

(14 reference statements)

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“…The human breast cancer cell lines MCF-7 and MDA-MB-231 were cultured in DMEM high glucose and Leibovitz’s-L15, respectively, supplemented with 10% ( v / v ) FBS [ 102 , 103 ]. The non-cancerous human breast cell line, MCF-10A, was cultured in DMEM/F12, supplemented with 5% ( v / v ) horse serum, 20 ng/mL of epidermal growth factor, 10 µg/mL of insulin, and 0.5 µg/mL of hydrocortisone.…”

Section: Methodsmentioning

confidence: 99%

Cannabinoid Combination Induces Cytoplasmic Vacuolation in MCF-7 Breast Cancer Cells

2020

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This study evaluated the synergistic anti-cancer potential of cannabinoid combinations across the MDA-MB-231 and MCF-7 human breast cancer cell lines. Cannabinoids were combined and their synergistic interactions were evaluated using median effect analysis. The most promising cannabinoid combination (C6) consisted of tetrahydrocannabinol, cannabigerol (CBG), cannabinol (CBN), and cannabidiol (CBD), and displayed favorable dose reduction indices and limited cytotoxicity against the non-cancerous breast cell line, MCF-10A. C6 exerted its effects in the MCF-7 cell line by inducing cell cycle arrest in the G2 phase, followed by the induction of apoptosis. Morphological observations indicated the induction of cytoplasmic vacuolation, with further investigation suggesting that the vacuole membrane was derived from the endoplasmic reticulum. In addition, lipid accumulation, increased lysosome size, and significant increases in the endoplasmic reticulum chaperone protein glucose-regulated protein 78 (GRP78) expression were also observed. The selectivity and ability of cannabinoids to halt cancer cell proliferation via pathways resembling apoptosis, autophagy, and paraptosis shows promise for cannabinoid use in standardized breast cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Cannabinoid Combination Induces Cytoplasmic Vacuolation in MCF-7 Breast Cancer Cells

2020

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“…The number of invading cells in vitro was measured through Matrigel‐coated transwell inserts (Costar, Cambridge, MA) as described previously . All assays were repeated at least three times independently.…”

Section: Methodsmentioning

confidence: 99%

MiR125a‐5p acting as a novel Gab2 suppressor inhibits invasion of glioma

Sun

Zhang

Liu

et al. 2015

Molecular Carcinogenesis

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Poor prognosis of glioma is due to the characteristics of high invasiveness. Recently, it was demonstrated that Gab2 was over-expressed and related to cellular migration and invasion in glioma, however, the mechanisms of regulation are still unknown. A better understanding of molecular events key to the carcinogenesis and tumor progression may facilitate development of new therapeutic targets and anti-glioma strategies. This study is the first to focus on miR125a-5p, which was predicted to regulate Gab2 with directly targeting the 3' un-translated region (3'UTR) of Gab2 and could inhibit migration and invasion of glioma cells by mediating Gab2 to affect cytoskeleton rearrangement and matrix metalloproteinases expression. Interestingly, further evaluation revealed that the miR125a-5p promoter was hypermethylated and that attenuating promoter methylation was sufficient to up-regulate miR125a-5p expression in glioma cells. Additionally, we reported that miR125a-5p was down-regulated in glioma as well as statistical analysis suggested that its expression level correlated with the World Health Organization grades of glioma (P < 0.05) and that patients with a low miR125a-5p level exhibited shorter survival time (P < 0.05). Taken together, these results reveal that miR125a-5p represents potential therapeutic targets in glioma by modulating Gab2.

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“…The anti-invasive effect of oxidized streptolysin O on MDA-MB231 breast cancer cells is characterized by its ability to activate the autophosphorylation of EGFR selectively on tyrosine residues besides Y845, indicating that, in this case, Y845 may not be involved in the anti-cancer malignancy [93]. On the other hand, Y845 phosphorylation has been identified as a diagnostic marker for the inhibitory effect of ipriflavone on osteolytic bone metastasis of the same cells in a nude mouse model [94].…”

Section: Y845 Phosphorylation and Cancer Cell Functionsmentioning

confidence: 99%

Cellular Functions Regulated by Phosphorylation of EGFR on Tyr845

Sato

2013

IJMS

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The Src gene product (Src) and the epidermal growth factor receptor (EGFR) are prototypes of oncogene products and function primarily as a cytoplasmic non-receptor tyrosine kinase and a transmembrane receptor tyrosine kinase, respectively. The identification of Src and EGFR, and the subsequent extensive investigations of these proteins have long provided cutting edge research in cancer and other molecular and cellular biological studies. In 1995, we reported that the human epidermoid carcinoma cells, A431, contain a small fraction of Src and EGFR in which these two kinase were in physical association with each other, and that Src phosphorylates EGFR on tyrosine 845 (Y845) in the Src-EGFR complex. Y845 of EGFR is located in the activation segment of the kinase domain, where many protein kinases contain kinase-activating autophosphorylation sites (e.g., cAMP-dependent protein kinase, Src family kinases, transmembrane receptor type tyrosine kinases) or trans-phosphorylation sites (e.g., cyclin-dependent protein kinase, mitogen-activated protein kinase, Akt protein kinase). A number of studies have demonstrated that Y845 phosphorylation serves an important role in cancer as well as normal cells. Here we compile the experimental facts involving Src phosphorylation of EGFR on Y845, by which cell proliferation, cell cycle control, mitochondrial regulation of cell metabolism, gamete activation and other cellular functions are regulated. We also discuss the physiological relevance, as well as structural insights of the Y845 phosphorylation.

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Inhibition of human breast cancer Matrigel invasion by Streptolysin O activation of the EGF receptor ErbB1

Cited by 9 publications

References 15 publications

Cannabinoid Combination Induces Cytoplasmic Vacuolation in MCF-7 Breast Cancer Cells

Cannabinoid Combination Induces Cytoplasmic Vacuolation in MCF-7 Breast Cancer Cells

MiR125a‐5p acting as a novel Gab2 suppressor inhibits invasion of glioma

Cellular Functions Regulated by Phosphorylation of EGFR on Tyr845

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