Inhibition of Herpes Simplex Virus Replication in vitro by Human Cytotoxic T Cell Clones and Natural Killer Cell Clones

Yasukawa, Masaki; Kobayashi, Yoshinari

doi:10.1099/0022-1317-66-10-2225

Cited by 19 publications

(36 citation statements)

References 16 publications

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“…The gD Ϫ18 to Ϫ10 , gD [53][54][55][56][57][58][59][60][61] , gD 70 -78 , and gD 278 -286 peptides had the highest affinity binding to HLA-A*0201 molecules (K d affinity values of 19 -62 nM). Of the remaining six peptides, gD 153-161 , gD 224 -232 , and gD showed medium affinity to HLA-A*0201 molecules (K d affinity values of 2002-4891 nM), whereas gD Ϫ13 to Ϫ5 , gD Ϫ11 to Ϫ2 , and gD 95-103 had low affinity (K d Ͼ 50,000 nM).…”

Section: In Silico Prediction and In Vitro Binding Of Potential Hsv-1mentioning

confidence: 99%

“…Three of the 10 predicted epitopes are located within the gD signal sequence (gD Ϫ13 to Ϫ5 , gD Ϫ11 to Ϫ2 , and gD Ϫ18 to Ϫ10 ). Four belong to the external N-terminal portion of gD (gD [53][54][55][56][57][58][59][60][61] , gD 70 -78 , gD [95][96][97][98][99][100][101][102][103] , and gD 153-161 ). Three are adjacent to the hydrophobic membrane anchor domain (gD 224 -232 , gD [253][254][255][256][257][258][259][260][261][262] , and gD 278 -286 ).…”

Section: In Silico Prediction and In Vitro Binding Of Potential Hsv-1mentioning

confidence: 99%

“…1). Of the four peptides previously showing the highest affinity binding to purified HLA-A*0201 molecules, gD [53][54][55][56][57][58][59][60][61] , gD 70 -78 , and gD 278 -286 peptides significantly increased levels of HLA-A*0201 molecules on the surface of the T 2 cells ( p Ͻ 0.005) in a dose-dependent manner (Fig. 1).…”

Section: High-affinity Gd Peptides Stabilized Hla-a*0201 Molecules Onmentioning

confidence: 99%

“…ϩ T cell assays, we found three CD8 ϩ T cell epitopes: gD [53][54][55][56][57][58][59][60][61] , gD 70 -78 , and gD 278 -286 . These minimal T cell epitopes are highly conserved among and between HSV-1 and HSV-2 strains.…”

mentioning

confidence: 99%

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HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D

Chentoufi

Zhang

Lamberth³

et al. 2008

The Journal of Immunology

140

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Evidence obtained from both animal models and humans suggests that T cells specific for HSV-1 and HSV-2 glycoprotein D (gD) contribute to protective immunity against herpes infection. However, knowledge of gD-specific human T cell responses is limited to CD4+ T cell epitopes, with no CD8+ T cell epitopes identified to date. In this study, we screened the HSV-1 gD amino acid sequence for HLA-A*0201-restricted epitopes using several predictive computational algorithms and identified 10 high probability CD8+ T cell epitopes. Synthetic peptides corresponding to four of these epitopes, each nine to 10 amino acids in length, exhibited high-affinity binding in vitro to purified human HLA-A*0201 molecules. Three of these four peptide epitopes, gD53–61, gD70–78, and gD278–286, significantly stabilized HLA-A*0201 molecules on T2 cell lines and are highly conserved among and between HSV-1 and HSV-2 strains. Consistent with this, in 33 sequentially studied HLA-A*0201-positive, HSV-1-seropositive, and/or HSV-2-seropositive healthy individuals, the most frequent and robust CD8+ T cell responses, assessed by IFN-γ ELISPOT, CD107a/b cytotoxic degranulation, and tetramer assays, were directed mainly against gD53–61, gD70–78, and gD278–286 epitopes. In addition, CD8+ T cell lines generated by gD53–61, gD70–78, and gD278–286 peptides recognized infected target cells expressing native gD. Lastly, CD8+ T cell responses specific to gD53–61, gD70–78, and gD278–286 epitopes were induced in HLA-A*0201 transgenic mice following ocular or genital infection with either HSV-1 or HSV-2. The functional gD CD8+ T cell epitopes described herein are potentially important components of clinical immunotherapeutic and immunoprophylactic herpes vaccines.

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Section: In Silico Prediction and In Vitro Binding Of Potential Hsv-1mentioning

confidence: 99%

Section: In Silico Prediction and In Vitro Binding Of Potential Hsv-1mentioning

confidence: 99%

Section: High-affinity Gd Peptides Stabilized Hla-a*0201 Molecules Onmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D

Chentoufi

Zhang

Lamberth³

et al. 2008

The Journal of Immunology

140

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“…7). We supposed that the viruses may be eliminated by the SNK-6 cells derived from the NK/T cell line which limited the replication of the viruses in vitro (23). These unexpected findings suggest that the SNK-6 cell line was unsuitable for use in the viral vector-mediated gene therapy, while the Hut 78 cell line should be an attractive target for viral therapy.…”

Section: Discussionmentioning

confidence: 92%

Evaluation of HSV-1 and adenovirus vector-mediated infection, replication and cytotoxicity in lymphoma cell lines

Zhang

Zhao²,

Hang³

et al. 2011

Oncol Rep

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Abstract. Lymphoma is not a common focus for viral therapy as many researchers do not consider lymphoma as a suitable target for viral vectors. In the present study, the infection, replication and cytotoxicity of herpes simplex virus (HSV) and adenovirus vectors were screened and evaluated in different lymphoma cell lines. Three recombinant viruses, BAC-HSV-1-eGFP, Adv-eGFP and an NV1020-like oncolytic HSV-1 virus strain named BAC-HSV-1-eGFP-delIRs, inserted with green fluorescent protein (GFP) as a reporter, were applied to evaluate the efficiency of viral infection and replication and cytotoxicity to lymphoma cells. Four types of lymphoma cell lines (SNK-6, Jurkat, Raji and Hut 78) were examined. We found that the HSV-1 vector, BAC-HSV-1-eGFP, was able to infect and replicate in the three lymphoma cell lines (Jurkat, Raji and Hut 78) and inhibit the growth of these cells more efficiently than adenovirus vector Adv-eGFP. The sensitivity of the four types of lymphoma cell lines to the viral vectors was different. The human cutaneous TL cell line Hut 78 was more sensitive to the viral vectors than the other cell lines. However, the human NK/T lymphoma cell line SNK-6 was not infected by any of the viral vectors and did not allow replication of the viruses. In conclusion, lymphoma may be a potential target for HSV-1 vector-mediated viral therapy.

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Natural Host Defense Systems Active against Herpes Simplex Virus Infections

López¹,

Fitzgerald-Bocarsly

1989

Functions of the Natural Immune System

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Inhibition of Herpes Simplex Virus Replication in vitro by Human Cytotoxic T Cell Clones and Natural Killer Cell Clones

Cited by 19 publications

References 16 publications

HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D

HLA-A*0201-Restricted CD8+ Cytotoxic T Lymphocyte Epitopes Identified from Herpes Simplex Virus Glycoprotein D

Evaluation of HSV-1 and adenovirus vector-mediated infection, replication and cytotoxicity in lymphoma cell lines

Natural Host Defense Systems Active against Herpes Simplex Virus Infections

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