Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by aqueous extracts and pectic polysaccharides from the roots of Cochlospermum tinctorium A. Rich. and Vernonia kotschyana Sch. Bip. ex Walp

Inngjerdingen, Kari Tvete; Thöle, Christian; Diallo, Drissa; Paulsen, Berit Smestad; Hensel, Andreas

doi:10.1016/j.fitote.2014.03.009

Cited by 28 publications

(25 citation statements)

References 32 publications

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“…Third, by upregulating the release of neutralizing cytokines such as IL-1Ra, a natural inhibitor of proinflammatory IL-1␤. Also, we demonstrated that dietary fibers, just like reported for probiotics [35], may contribute to shift in T-cell polarization as will be discussed in more detail below.…”

Section: Discussionsupporting

confidence: 57%

“…Gut mucosal DCs, especially the CX3CR1 + DCs are considered to be involved in sampling luminal antigens for priming immune activation or tolerance . We decided to study direct effects on our human DCs as it has been shown that dietary fibers are able to pass the intestinal barrier and encounter DCs in the lamina propria and in the systemic circulation directly . These direct effects of dietary fibers on DCs were rather minor.…”

Section: Discussionmentioning

confidence: 99%

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The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

Bermúdez-Brito

Sahasrabudhe

Rösch

et al. 2015

Molecular Nutrition Food Res

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

Bermúdez-Brito

Sahasrabudhe

Rösch

et al. 2015

Molecular Nutrition Food Res

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“…The pharmacological activity of certain plants, such as the Asteraceae family, are attributed to the presence of sesquiterpenes[21, 23–25]. Other bioactive compounds with anti-inflammatory effects have been isolated from the Vernonia genus, such as flavonoids[26], steroid glicoside[27, 28], polysaccharides[29, 30] and chalconoids[31].…”

Section: Discussionmentioning

confidence: 99%

Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells

et al. 2019

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Species of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2 than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription.

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“…[ 2 ] Similarly, the attachment of Helicobacter pylori to human gastric cells can be inhibited by water extracts of Vernonia kotschyana or Cochlospermum tinctorium . [ 3 ] Moreover, Rhodomyrtus tomentosa leaf extract has demonstrated inhibitory activity of the adhesion of pathogen microorganisms to buccal cells. [ 4 ]…”

Section: Introductionmentioning

confidence: 99%

Extracts from Flammulina velutipes inhibit the adhesion of pathogenic fungi to epithelial cells

et al. 2016

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Background:Recently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch-chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion.Objective:In this study, we assessed the capacity of extracts from F. velutipes (wild-type AQF-1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells.Materials and Methods:The aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 μg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin-FITC.Results:The extract from strain AQF-1 (50, 100 and 150 μg/mL) inhibited the adhesion of: S. schenkii in a dose-dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose-independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans.Conclusion:The extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed.SUMMARY The yields of mycelium from two strains of F. velutipes and the extract from it were similar.Extracts from both strains have inhibited adhesion of S. schenkii and C. albicans to epithelial cells in vitro, but the extract from strain AQF-1 was more effective.The extracts have not prevented damage to epithelial cells caused by pathogenic fungi. Abbreviation Used: YPG: Yeast peptone glucose, DMEM: Dulbecco's Modified Eagle's medium, FITC: Fluorescein isothiocyanate

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Inhibition of Helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by aqueous extracts and pectic polysaccharides from the roots of Cochlospermum tinctorium A. Rich. and Vernonia kotschyana Sch. Bip. ex Walp

Cited by 28 publications

References 32 publications

The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

The impact of dietary fibers on dendritic cell responses in vitro is dependent on the differential effects of the fibers on intestinal epithelial cells

Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells

Extracts from Flammulina velutipes inhibit the adhesion of pathogenic fungi to epithelial cells

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