Inhibition of growth of nonbasal planes in ice by fish antifreezes.

Raymond, James A.; Wilson, Peter W.; DeVries, Arthur L.

doi:10.1073/pnas.86.3.881

Cited by 148 publications

(96 citation statements)

References 26 publications

(27 reference statements)

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“…All the classical MD simulations were performed on the systems in canonical (NVT) ensemble with the GROMACS software package [32]. Following equilibration, trajectories of 2 ns were obtained at 300 K with a Nose-Hoover thermostat [22,23]. Nonbonded interactions were gradually brought to zero by a shift function for the electrostatics as well as a switch function for van der Waals interactions between 10 and 12Å [24,25].…”

Section: Methodsmentioning

confidence: 99%

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Analysis of Water and Hydrogen Bond Dynamics at the Surface of an Antifreeze Protein

Gnanasekaran

Leitner

2012

Journal of Atomic, Molecular, and Optical Physics

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We examine dynamics of water molecules and hydrogen bonds at the water-protein interface of the wild-type antifreeze protein from spruce budworm Choristoneura fumiferana and a mutant that is not antifreeze active by all-atom molecular dynamics simulations. Water dynamics in the hydration layer around the protein is analyzed by calculation of velocity autocorrelation functions and their power spectra, and hydrogen bond time correlation functions are calculated for hydrogen bonds between water molecules and the protein. Both water and hydrogen bond dynamics from subpicosecond to hundred picosecond time scales are sensitive to location on the protein surface and appear correlated with protein function. In particular, hydrogen bond lifetimes are longest for water molecules hydrogen bonded to the ice-binding plane of the wild type, whereas hydrogen bond lifetimes between water and protein atoms on all three planes are similar for the mutant.

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Section: Methodsmentioning

confidence: 99%

“…The generally accepted mechanism for the Type I AFP is the adsorption-inhibition mechanism [22][23][24], which proposes that AFPs adsorb onto the preferred growth sites of an ice surface, thereby preventing new ice growth [25]. It was initially thought that ice and AFP interacted through hydrogen bonding [22].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Water and Hydrogen Bond Dynamics at the Surface of an Antifreeze Protein

Gnanasekaran

Leitner

2012

Journal of Atomic, Molecular, and Optical Physics

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“…This has been attributed to a secondary property of AF(G)Ps known as dynamic ice shaping (DIS), which is intrinsically linked to their TH property. Essentially, AF(G)Ps bind to specific planes of ice, inhibiting their growth, but upon cooling, the remaining exposed faces grow, giving rise to a spicular (needle-like) morphology causing mechanical damage 17 . Carpenter et al 11 and Matsumoto et al 18 demonstrated small improvements in cryopreservation with an AFP and an AF(G)P mimic respectively, but the benefit was limited by the low concentrations, which could be applied before cell viability was reduced because of ice-shaping.…”

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confidence: 99%

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

et al. 2014

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The cryopreservation of cells, tissue and organs is fundamental to modern biotechnology, transplantation medicine and chemical biology. The current state-of-the-art method of cryopreservation is the addition of large amounts of organic solvents such as glycerol or dimethyl sulfoxide, to promote vitrification and prevent ice formation. Here we employ a synthetic, biomimetic, polymer, which is capable of slowing the growth of ice crystals in a manner similar to antifreeze (glyco)proteins to enhance the cryopreservation of sheep and human red blood cells. We find that only 0.1 wt% of the polymer is required to attain significant cell recovery post freezing, compared with over 20 wt% required for solvent-based strategies. These results demonstrate that synthetic antifreeze (glyco)protein mimics could have a crucial role in modern regenerative medicine to improve the storage and distribution of biological material for transplantation.

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“…The data also revealed that the low molecular weight AFGP 6-8 are less active than the high molecular weight AFGP 1-5. Also, the thermograms of AFGP revealed an initial shoulder in the exotherm direction upon cooling which correlates with observed c-axis ice growth (Raymond et al 1989, Hansen et al 1991; and, the loss of the shoulder during annealing experiments represents the end of the ice growth.…”

Section: Discussionmentioning

confidence: 91%

Antifreeze glycopeptides and peptides in Antarctic fish species from the Weddell Sea and the Lazarev Sea

Wöhrmann¹

1996

Mar. Ecol. Prog. Ser.

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Antifreeze glycopeptides and peptides have been isolated from 37 species of Antarctic fish representing the families Nototheniidae, Artedidraconidae, Bathydraconidae, Channichthyidae, Muraenolepididae. Liparididae, Zoarcidae and Myctophidae. Amino acid and carbohydrate analysis as well as antifreeze activity indicate that all investigated notothenioids contain antifreeze glycopeptides [AFGP). Pleuragramma antarcticurn, Lepidonotothen kempi, Bathydraco rnarri and Dolloidraco longedorsalis synthesize additional antifreeze molecules. The non-notothenioid species possess antifreeze peptides (AFP), except Muraenolepis marrnoratus and Macrourus holotrachys, which possess a glycosylated antifreeze pept~de similar to the AFGP found in the notothenio~d species. A novel glycopeptide comprised of the carbohydrate residue N-acetylglucosamine and the amino acids asparagine, glutamine, glycine, alanine, and traces of arginine, valine, leucine and threonine was isolated and characterized from P. antarcticum. The level of antifreeze concentration was dependent on the ambient water temperature, the depth of catch and life cycle of the species. Antifreeze activity of AFGP varies between 0.52 (Neopagetopsis ionah) and 1.20°C ( P antarctjcum) at a concentration of 20 mg ml-' Antifreeze activity of AFP is lower than 0.50°C. A linear increase in activity of the AFGP could be demonstrated concomitant with decreasing ice content. The structural diversity of antifreeze molecules and their occurrence in a wide range of Arctic and Antarctic fish species suggest that they evolved from precursor proteins before the continental drift and recently during Cenozoic glaciation into the various antifreeze molecules.

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Inhibition of growth of nonbasal planes in ice by fish antifreezes.

Cited by 148 publications

References 26 publications

Analysis of Water and Hydrogen Bond Dynamics at the Surface of an Antifreeze Protein

Analysis of Water and Hydrogen Bond Dynamics at the Surface of an Antifreeze Protein

Synthetic polymers enable non-vitreous cellular cryopreservation by reducing ice crystal growth during thawing

Antifreeze glycopeptides and peptides in Antarctic fish species from the Weddell Sea and the Lazarev Sea

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