Inhibition of generalized wound infection in immunized rats

Ai, Shchegolev; Od, Mishnev

doi:10.1007/bf02444314

Cited by 3 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of bacterial cells in the control remained unchanged. The early signs of bacteriopyknosis, i.e., conversion of bacteria into detritus [3,4], were seen. There were intermediate forms between bacteria and detritus, i.e., bacterial cells with pronounced deformations (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Some animals were sacrificed 0007-4888/96/0009-0956515.00 9 1997 Plenum Publishing Corporation under ketamine narcosis 0.5 to 22 h after the injection, and the sites of infection were studied morphologically. The antibody titer was determined by the agglutination reaction [3]. The significance of differences was evaluated by the Wilcoxon t e s t .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Suppression of phagocytosis in immunized animals

et al. 1996

Self Cite

View full text Add to dashboard Cite

It is found that phagocytosis in animals immunized with Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis is suppressed in comparison with intact animals, and the degree of suppression rises with the antibody titer. Morphological study of the site of infection shows that antibodies markedly enhance bacteriopyknosis, i.e., convert bacteria into nonphagocytizable detritus. Focal suppression of phagocytosis in immunized animals is attributed to rapid bacteriopyknosis and, consequently, to a sharp decrease in the number of intact bacteria that can be digested by phagocytes. Key Words: infectious immunity; antibodies; phagocytosis; nonspecific organism's resistanceEnhanced phagocytosis in immunized individuals has been considered as the main mechanism of antibacterial immunity. This enhancement is provided by antibodies [1,2,5,9]. Antibodies mediate bacterial binding to the phagocyte surface with subsequent formation of the antibody-bacterium complexes which trigger the classical pathway of complement activation. As a result, bacteria bind to phagocytes via the receptors for antibodies and complement [6,10], Opsonization, i.e., the phagocyte-stimulating effect of antibodies, was confirmed by in vitro experiments: absorption and killing of bacteria by neutrophils and macrophages is considerably increased in the presence of antibodies. Systemic administration of antibodies often produces a protective effect and alleviates the disease. The protective effect of antibodies in vivo and the opsonizing effect in vitro provide the basis for the modern concept of infectious immunity. This theory has been confirmed by considerable experimental and clinical data accumulated during the last century; however, there is evidence contradicting it. It was found that despite the pronounced opsonizing effect, antibodies elicit no protective effect in some experimental infections [7,8,11]. These reports, however, did not Here we report a simple experiment demonstrating that phagocytosis in vivo, in contrast to that in vitro, is inhibited by antibodies and make an attempt to explain this phenomenon. MATERIALS AND METHODSExperiments were carried out on nonpedigree rats weighing 170-300 g. The animals were infected with one-day-old agar cultures of Pseudomonas aeruginosa (strain 453), Staphylococcus aureus (strains 3377 and 3384), and Staphylococcus epidermidis (strain 4017) isolated from burns. The bacteria were suspended in Hanks' solution, and 0.3 ml of this suspension was injected into the gastrocnemius muscle. The intensity of phagocytosis was assessed by the mass of exudate in the site of injection after 7 days. All necrotic detritus from the site of infection was collected and weighted. For active immunization the same cultures were injected into rats in a 10-fold lower dose 3 times at one-week intervals. Immunized and control (intact) rats were infected simultaneously with the same bacterial suspension one week after the last immunization. Some animals were sacrificed

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Suppression of phagocytosis in immunized animals

et al. 1996

Self Cite

View full text Add to dashboard Cite

show abstract

“…The intensity of bacteriopyknosis was estimated by the mass of purulent-necrotic exudate formed in the site of injection 7 days after injection of microbial suspension. Previously, it was demonstrated that bacteriopyknosis inhibits phagocytosis and reduces the size of abscess [1,2]. The significance of differences was evaluated by Wilcoxon's test.…”

Section: Methodsmentioning

confidence: 99%

“…A relationship between erythrocyte damage and progressive microcirculatory disorders upon artificial [1,5,7] and assisted circulation [6] has been established. However, the information regarding the effect of direct cardiac massage (DCM) on peripheral blood erythrocytes is scanty.…”

Section: Abstract: Direct Cardiac Massage; Erythrocyte Morphology; Smentioning

confidence: 99%

See 1 more Smart Citation

On the mechanisms of bacteriopyknosis

et al. 1997

Self Cite

View full text Add to dashboard Cite

Morphological examination of primary foci several hours after intramuscular infection of rats with Pseudomonas aeruginosa, Staphylococcus aureus, or Staphylococcus epidermidis showed that bacteriopyknosis is associated with deficiency of compounds necessary for the microorganisms or excess of secreted microbial metabolites. Motility is a factor of virulence, since it inhibits bacteriopyknosis. The biological significance of divalent antibodies and agglutination provided by them probably lies in considerable stimulation of bacteriopyknosis in agglutinates. Formation of IgM also enhances bacteriopyknosis.

show abstract

The role of agglutination in bacterial infection

et al. 1999

Self Cite

View full text Add to dashboard Cite

The process of concentration of microorganisms, which leads to inhibition of their growth and death, is a mechanism of nonspecific resistance and immunity in bacterial infection. It was suggested that inhibition and death of microorganisms are caused by deficiency of essential substances in the concentration site, which determines the significance of agglutination in the immunity. Agglutination of microbial bodies increases their concentration in tissues. By contrast, dispersion of bacteria in the tissues weakens the effect of the concentration factor and, consequently, increases the virulence of the bacteria.

show abstract

Inhibition of generalized wound infection in immunized rats

Cited by 3 publications

References 6 publications

Suppression of phagocytosis in immunized animals

Suppression of phagocytosis in immunized animals

On the mechanisms of bacteriopyknosis

The role of agglutination in bacterial infection

Contact Info

Product

Resources

About