Inhibition of Gastric H⁺,K⁺-ATPase andHelicobacter pyloriGrowth by Phenolic Antioxidants ofCurcuma amada

Abstract

Gastric ulcer is the most prevalent gastrointestinal disorder, resulting from oxidative stress, Helicobacter pylori infection, up-regulation of proton potassium ATPase (PPA) activity, down-regulation of gastric mucosal defense, etc. In this paper it is reported that phenolic fractions of Curcuma amada, commonly known as mango ginger, acted as potent inhibitors of PPA and H. pylori growth. Mango ginger free phenolics (MGFP) and mango ginger bound phenolics (MGBP) inhibited PPA at IC50 values of 2.2 +/- 0.21 and 0.7 +/- 0.08 microg/mL, respectively, exhibiting 9-27-fold better potency over lansoprazole (IC(50) of 19.3 +/- 2.2 microg/mL). MGFP is constituted by caffeic (26%), gentisic (24%), ferulic (20%), gallic (10%), cinnamic (7%), and protocatechuic acids (7%) and MGBP by ferulic (47%), cinnamic (29%), p-coumaric acid (11%), and syringic (5%) acids as major phenolic acids. MGFP and MGBP further exhibited free radical scavenging (IC(50) of 2.2 +/- 0.17 and 4.2 +/- 0.36 microg/mL), reducing power abilities (193-104 units/g), inhibition of lipid peroxidation (IC(50) of 10.3 +/- 0.91 and 15.6 +/- 1.6 microg/mL), and DNA protection (80% at 4 microg), indicating strong antioxidative properties. MGFP and MGBP thus may be potential and inexpensive multistep blockers against ulcers.

Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds

“…A recent study has shown that the phenolic antioxidants of Curcuma amada (popularly known as mango ginger), which possesses antioxidative and antimicrobial properties against H. pylori, were capable of inhibiting the human gastric H + ,K + -ATPase activity that is responsible for acid secretion [59].…”

Mango ginger (Curcuma amada Roxb.) – A promising spice for phytochemicals and biological activities

“…Antibacterial activity of free and bound phenolics from mango ginger rhizomes has been reported by Siddaraju and Dharmesh (2007). Both free and bound phenolic fractions of mango ginger are effective in inhibiting H+, K+-ATPase activity and Helicobacter pylori growth.…”

“…The free phenolic acids (figure 4) present in mango ginger are caffeic (26%, 195 mg/g), gentisic (24%, 180 mg/g) and ferulic (20%, 150 mg/g) followed by gallic (10%, 75 mg/g), cinnamic (7%, 52.5 mg/g), protocatechuic (7%, 52.5 mg/g) and small amounts of syringic (4%, 30 mg/g) and p-coumaric acids (2%, 15 mg/g) (Siddaraju and Dharmesh 2007). It also contains bound phenolic compounds like ferulic acid (47%, 391.5 mg/g) and cinnamic acid (29%, 237 mg/g), p-coumaric acid (11%, 95 mg/g), syringic acid (5%, 38.8 mg/g), caffeic acid (4%, 30.7 mg/g), gallic acid (1%, 11.5 mg/g) and gentisic acid (1%, 4.9 mg/g).…”

Nutritional value, phytochemical composition, and biological activities of edibleCurcumaspecies: A review

“…Biological activities Curcuma aeruginosa Antimicrobial [164] , cytotoxicity [152] ; antinociceptive, antipyretic [13] ; uterine relaxant [74] ; antioxidant [165] ; inhibition of nitric oxide production [166] ; and anti-inflammatory [153] Curcuma amada Antifungal and insecticidal [79] ; CNS depressant and analgesic [16,167] ; antiulcer [168] ; anti-tubercular [135] ; antioxidant, antimicrobial, cytotoxic and antiplatelet [20] ; anti-inflammatory [169] ; anticancer [170] ; and larvicidal [171] Curcuma angustifolia Antimicrobial, antiulcerogenic [172,173] ; antioxidant [155] ; antifungal and antibacterial [129] Curcuma aromatica Hypoglycemic [172] ; anti-Alzheimer [173] ; antioxidant [2] ; cytotoxicity [174] ; insecticidal [175] ; antiinflammatory [169] ; antibacterial [11] ; inhibition of nitric oxide production [176] ; antiproliferative [177] ; and gastroprotective [21] Curcuma australasica Inhibition of nitric oxide and prostaglandin production, anti-inflammatory, and cytotoxic [115] Curcuma caesia Smooth muscle relaxant [178] ; anxiolytic, CNS depressant and neuropharmacological [179,180] ; antimicrobial [151] ; antiulcerogenic [181] ; antitumor and antioxidant [116] ; anticancer [182] ; antihepatotoxicity and nephrotoxicity [...…”