Inhibition of fluorescent advanced glycation end products (AGEs) of human serum albumin upon incubation with 3-β-hydroxybutyrate

Bohlooli, Mousa; Moosavi‐Movahedi, Ali Akbar; Taghavi, Fereshteh; Saboury, Ali Akbar; Maghami, Parvaneh; Seyedarabi, Arefeh; Moosavi-Movahedi, Faezeh; Ahmad, Faizan; Shockravi, Abbas; Habibi-Rezaei, Mehran

doi:10.1007/s11033-014-3235-1

Cited by 18 publications

(13 citation statements)

References 43 publications

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“…However, the concentration of these species in serum and thereby their emission are very low; therefore, the fluorescence of advanced glycation end products (AGEs) having similar spectra was suggested by Münch [19]. Recently, studies on AGEs of human serum albumin by means of circular dichroism, fluorescence spectroscopy and differential scanning calorimetry have been reported by Bohlooli et al [20]. The fluorescence spectra at 325 and 365 nm obtained in our study seem to be similar to those from glycated human serum.…”

Section: Methodsmentioning

confidence: 99%

Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

et al. 2016

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The paper presents an investigation into the effects of endurance training and testosterone enanthate (TE) treatment on rat blood serum by fluorescence spectroscopy. Blood serum was collected from adolescent male Wistar rats randomized between the untrained controls and trained animals, either steroid untreated or receiving I.M. injections (8 or 80 mg/kgBW) of testosterone enanthate. Fluorescence measurements were performed for undiluted or 20-and 500-fold diluted serum and analysed separately in the ultraviolet and visible light regions. In both regions the impact of the TE administration was more pronounced than the effect of endurance training. Moreover, in the visible region of spectrum, originated from a number of fluorescent metabolites, the influence of studied factors was more noticeable. The opposite effect of endurance training and administration of TE on serum emission was found for a lower (8 mg/kgBW) dose of testosterone, whereas a high-dose TE (80 mg/kgBW) treatment resulted in a return of fluorescence intensity.

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Section: Methodsmentioning

confidence: 99%

Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

et al. 2016

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“…There are many compounds which have been reported as inhibitors of the glycation process with potential health benefits . Recently, the 3‐β‐hydroxybutyrate (3BHB) has been studied as an inhibitor of fluorescence AGEs formation . It is produced by the liver as a ketone body .…”

Section: Introductionmentioning

confidence: 99%

Determination of Amadori Product in Glycated Human Serum Albumin by Spectroscopy Methods

2018

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The Amadori product is a stable intermediate in the glycation process, and its increased formation is a marker of hyperglycemia in diabetes mellitus. Its accumulation in the body contributes to pathological complications of diabetes including diabetic nephropathy and retinopathy, and cardiovascular diseases. In this study the effect of 3‐β‐hydroxybutyrate on the production of Amadori products and structural changes of human serum albumin (HSA) after incubation with glucose was investigated using FT‐IR, circular dichroism (CD), and UV‐visible and fluorescence spectroscopy. Our results showed that the production of Amadori products in HSA incubated with glucose was decreased in the presence of 3‐β‐hydroxybutyrate. The glycation‐mediated alterations in HSA secondary and tertiary structures were also reduced in the presence of 3‐β‐hydroxybutyrate. These changes were attributed to reduced formation of glucose covalent bonds with the HSA lysine residues in the presence of 3‐β‐hydroxybutyrate. Thus, 3‐β‐hydroxybutyrate reduced the production of Amadori products by reacting with lysine residues and decreasing HSA covalent modifications by glucose.

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“…This binding causes extensive protein structural changes [7]. The results of free lysine content assay (TNBSA test) showed that the amount of modified lysine residues in HSA incubated with AA + Glc was significantly greater than that of HSA incubated with Glc and AA alone (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The third step is the irreversible stage, which produces the AGEs through oxidation, dehydration and cyclization [5]. AGEs are yellow-brown, often fluorescent (some are non-fluorescent), intra- or inter-molecular cross-linked, and insoluble compounds [6], which contribute to pathogenesis of many diseases [7]. …”

Section: Introductionmentioning

confidence: 99%

The role of acetoacetate in Amadori product formation of human serum albumin

Bohlooli

Ghaffari‐Moghaddam

Khajeh

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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Amadori product is an important and stable intermediate, which is produced during glycation process. It is a marker of hyperglycemia in diabetes mellitus, and its accumulation in the body contributes to microvascular complication of diabetes including diabetic nephropathy and retinopathy. In this study, the effect of acetoacetate on the formation of Amadori products and biophysical properties of human serum albumin (HSA), after incubation with glucose, was investigated using various methods. These included circular dichroism (CD), Fourier transform infrared (FTIR) spectroscopy, and UV–visible and fluorescence spectroscopy. Our results indicated that the production of Amadori products in HSA incubated with glucose (GHSA) was increased in the presence of acetoacetate. We also detected alterations in the secondary and tertiary structure of GHSA, which was increased in the presence of acetoacetate. These changes were attributed to the formation of covalent bonds between the carbonyl group of acetoacetate and the nucleophilic groups (lysine residues) of HSA. Thus, acetoacetate can enhance the production of Amadori products through formation of covalent bonds with biomaterials.

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Inhibition of fluorescent advanced glycation end products (AGEs) of human serum albumin upon incubation with 3-β-hydroxybutyrate

Cited by 18 publications

References 43 publications

Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

Determination of Amadori Product in Glycated Human Serum Albumin by Spectroscopy Methods

The role of acetoacetate in Amadori product formation of human serum albumin

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