Effect of Endurance Training and Testosterone Treatment on the Fluorescence Spectra of Rat Serum

Abstract

The paper presents an investigation into the effects of endurance training and testosterone enanthate (TE) treatment on rat blood serum by fluorescence spectroscopy. Blood serum was collected from adolescent male Wistar rats randomized between the untrained controls and trained animals, either steroid untreated or receiving I.M. injections (8 or 80 mg/kgBW) of testosterone enanthate. Fluorescence measurements were performed for undiluted or 20-and 500-fold diluted serum and analysed separately in the ultraviolet and visible light regions. In both regions the impact of the TE administration was more pronounced than the effect of endurance training. Moreover, in the visible region of spectrum, originated from a number of fluorescent metabolites, the influence of studied factors was more noticeable. The opposite effect of endurance training and administration of TE on serum emission was found for a lower (8 mg/kgBW) dose of testosterone, whereas a high-dose TE (80 mg/kgBW) treatment resulted in a return of fluorescence intensity.

Serum Autofluorescence and Biochemical Markers in Athlete’s Response to Strength Effort in Normobaric Hypoxia: A Preliminary Study

“…The main fluorescent species of serum have tentatively been identified by comparison with literature data as tryptophan (Trp), tyrosine (Tyr), reduced nicotinamide adenine dinucleotide (NADH), and its phosphate, pyridoxic acid lactone, pyridoxal phosphate Schiff base, and protein-bound bilirubin [36]. Their emission could be detected in two well-separated parts in the ultraviolet and visible regions (see Figures 1(a) and 1(b)), similarly as it was reported earlier for human and rat serum by Wolfbeis and Leiner [36] and Drzazga et al [39], respectively.…”

“…There are also reports demonstrating that fluorescence studies of serum can be potentially useful in distinguishing deviations from the normal status of serum in various types of disease [36–38]. Recently, the impact of endurance training and testosterone treatment on rat blood serum using fluorescence spectroscopy has been determined [39].…”