Inhibition of ferricyanide reduction in chloroplast particles by anaerobicity

King, Dan; Erbes, David L.; Gibbs, Martin

doi:10.1016/0006-291x(77)90240-6

Cited by 5 publications

(1 citation statement)

References 4 publications

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“…In this formulation, reduced pyridine nucleotides feed electrons into a component (plastoquinone) between the two photosystems that would account for H2 release insensitive to DCMU. There are two reports (23,29), however, describing inactivation of PSII activity after anaerobic incubation ofintact cells and thylakoidal preparations of C. reinhardii wt and F-60. The inhibitory effect of anaerobiosis remains unexplained but would account for the lack of 02 evolution by our cells.…”

Section: Methodsmentioning

confidence: 99%

Fermentative Metabolism of Chlamydomonas reinhardii

Gibbs¹,

Gfeller²,

Chen³

1986

Plant Physiol.

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The anaerobic photodissimilation of acetate by Chlamydomonas reinhardii F-60 adapted to a hydrogen metabolism was studied utilizing manometric and isotopic techniques. The rate of photoanaerobic (N2) acetate uptake was approximately 20 moles per milligram chlorophyll per hour or one-half that of the photoaerobic (air) rate. Under N2, cells produced 1.7 moles H2 and 0.8 mole CO2 per mole of acetate consumed. Gas production and acetate uptake were inhibited by monofluoroacetic acid (MFA), 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) and by H2. Acetate uptake was inhibited about 50% by 5% H2 (95% N2 (3) suggested that acetate increased gas production by consuming ATP which regulated the unspecified sequence of reactions giving rise to CO2 and H2. Healey (18) modifying a mechanism put forward by Jones and Myers (20) to explain the Kok effect in blue-green algae, proposed a flow of electrons from acetate via the citric acid cycle into PSI resulting in the photoevolution of H2 from reduced Fd. The operation of an anaerobic and light-dependent citric acid cycle which affects the stoichiometric conversion of acetate to CO2 and H2 had been documented in the photosynthetic purple bacteria ( 13).The present communication summarizes the results of a detailed investigation of the anaerobic photometabolism of acetate by C. reinhardii F-60, with reference to stoichiometry of gas (CO2 and H2) production, incorporation into cellular components, and sensitivity of the process to a variety of inhibitors. The stoichiometric relationships observed, together with the isotopic distribution following assimilation of ["4C]acetate, constitute strong evidence for the conclusion that anaerobic carbon oxidation occurs in part through the reactions of the glyoxylate and citric acid cycles. MATERIALS AND METHODSAlgal Growth Conditions. Chlamydomonas reinhardii (Dangeard) F-60, a mutant strain with an incomplete photosynthetic carbon reduction cycle but with an intact photosynthetic electron transport chain, was obtained from R. K. Togasaki, Indiana University. Cells were grown in batch cultures on an acetatesupplemented medium (14)

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Section: Methodsmentioning

confidence: 99%

Fermentative Metabolism of Chlamydomonas reinhardii

Gibbs¹,

Gfeller²,

Chen³

1986

Plant Physiol.

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Chemotactic Receptors of Dictyostelium discoideum

Frazier

Nandini-Kishore

Meyers

1982

J of Cellular Biochemistry

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For over 30 years, the cellular slime molds, and in particular the species Dictyostelium discoideum, have been viewed as a eukaryotic model system for the study of a variety of regulatory and developmental processes [l]. In particular, the transition from the free-living amoeboid stage to the multicellular slug stage has been the focus of a great deal of attention by those investigators studying the regulation of development [ 1-31. The first morphological evidence that differentiation of a starved population of cells is proceeding on schedule is the appearance of ripples or streams in a lawn of cells resting on a moist surface [l-31. This prelude to the readily identified tight aggregate stage indicates the functioning of the cells' endogenous chemosensory system. In 1969, Bonner and coworkers [4] identified the endogenously produced chemoattractant or acrasin of these cells as cyclic AMP (CAMP). While cAMP is widely encountered as an intracellular regulatory agent or second messenger, the Dictyostelia provide one of the few well documented examples of cAMP as an extracellular messenger molecule.The Polysphondylia such as P violaceum and P pallidum appear to employ a nonnucleotide molecule as their acrasin during starvation induced chemotaxis. This compound has been studied by Bonner and coworkers [S] and has been tentatively identified as a peptide with a blocked amino terminus, although further characterization is required for unambiguous identification. Bonner's group has also been responsible for the identification of folic acid and pterin compounds as chemoattractants of vegetative Dictyostelium amoebae [6-81. This identification of a nonacrasin chemoattractant raises the possibility of studying the transition from a vegetative to a developmental mode of chemotaxis. DEVELOPMENTAL CHEMOTAXIS IN D discoideumThe phenomenology of the CAMP chemotactic system in D discoideum has been well established [9,10]. It is convenient for descriptive purposes to imagine

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Hydrogen Metabolism

Ben‐Amotz¹

1979

Photosynthesis II

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Inhibition of ferricyanide reduction in chloroplast particles by anaerobicity

Cited by 5 publications

References 4 publications

Fermentative Metabolism of Chlamydomonas reinhardii

Fermentative Metabolism of Chlamydomonas reinhardii

Chemotactic Receptors of Dictyostelium discoideum

Hydrogen Metabolism

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