Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro

Abstract

In the present study, the effect of fatty acid synthase (FASN) inhibition on cell invasion and migration in vitro was investigated. A recombinant plasmid containing a microRNA targeting the FASN gene was used to inhibit FASN expression in U2‑OS cells. Cell migration and invasion were investigated using wound healing and Transwell invasion assays. We found that cell invasion and migration were suppressed by inhibiting FASN. In addition, the effect of inhibition of FASN on phosphorylation of Akt was investigated by detecting the expression levels of pAkt using western blot analysis. Furthermore, protein expression levels of nuclear factor‑κB (NF‑κB; p65) and matrix metalloproteinase (MMP)‑2 and ‑9 were also measured by western blot analysis. Results demonstrated that expression levels of pAkt, NF‑κB (p65) and MMP‑2 and ‑9 proteins were reduced significantly by inhibiting FASN. Therefore, we confirmed that inhibition of FASN by RNA interference suppresses osteosarcoma cell metastasis via downregulation of the phosphoinositide 3‑kinase/Akt/NF‑κB signaling pathway in vitro.

Podocalyxin promotes cisplatin chemoresistance in osteosarcoma cells through phosphatidylinositide 3-kinase signaling

“…A B OS cells. These findings are also concordant with those of previous studies, which indicated that the PI3K/Akt pathway has a critical role in cancer chemoresistance (22)(23)(24)(25). Of note, the selective PI3K inhibitor only blocked ~50% of the effect of PCX on cisplatin chemoresistance and cell survival in OS cells, suggesting the involvement of other signaling pathways, which may be identified in further studies.…”

“…Previous studies have demonstrated that the PI3K/Akt pathway is important for cancer cell chemoresistance (22)(23)(24)(25). Therefore, the present study examined the effects of PCX on the activity of PI3K and phosphorylation of Akt in OS cells.…”

Protective effect of proanthocyanidins on anoxia-reoxygenation injury of myocardial cells mediated by the PI3K/Akt/GSK-3β pathway and mitochondrial ATP-sensitive potassium channel

“…The protective effect of ischemic pretreatment on the myocardium is predominantly due to the activation of the PI3K/Akt signal transduction pathway (22). Efthymiou et al (23) identified that administering atorvastatin reduced the myocardial infarction area (MI) in the ischemia reperfusion model of the normal myocardium of rats in vitro.…”

Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells <i>via</i> the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury

“…It was also determined that the migratory ability of OECs was governed by the expression of migrationrelated factors, such as MMPs and NCAM1. MMP-2 and MMP-9 play important roles in the migration and invasion of various cells, 24) including OECs. 25,26) Secreted MMPs regulate the activity of the membrane, which is important for both cell migration and axonal regeneration.…”