Inhibition of DNA synthesis by Carvacrol in mouse myoblast cells bearing a human N-RAS oncogene

Zeytinoğlu, Hülya; İncesu, Zerrin; Başer, K. H. C.

doi:10.1078/094471103322004785

Cited by 88 publications

(57 citation statements)

References 24 publications

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“…The agents that alter the cell cycle have been of particular interest since cell cycle regulation was the basic mechanism underlying cell fate, i.e. proliferation, differentiation or apoptosis [29]. These results are in accordance with the previous results [30,31].…”

Section: Caspase-3 Activity Assaysupporting

confidence: 82%

Induction of Caspase-3 Dependent Apoptosis, Cell Cycle Arrest and Cytotoxicity in Breast Cancer Cells by Abrus Precatorius

Sofi

Nabi

2018

Int J Pharm Pharm Sci

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Objective: To evaluate the leaf extract of Abrus precatorius as a potential therapy for breast cancer treatment.Methods: Aqueous leaf extracts of A. precatorius was prepared by the process of maceration. The collected filtrate was further partitioned successively into five solvent fractions starting from water, hexane, chloroform, ethyl acetate and butanol by solvent fractionation method using separating funnel. Finally, all the five fractions were subjected to cytotoxic activity assay. The molecular mechanism underlying cytotoxicity was determined by using various approaches viz., 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Fluorescence-activated cell sorting (FACS) analysis, Western blot analysis, casp-glow assay and Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. Results: Among five fractions only ethyl acetate fraction of A. precatorius (EAF-AP) showed significant cytotoxic activity on MDA-MB-231 cells, with an IC50Conclusion: All these results indicate that EAF-AP, induced apoptosis in MDA-MB-231 cells, making A. precatorius a potentially good candidate for anticancer drug development.value of 47.3 µg/ml. Apoptosis was confirmed by the appearance of Sub G0/G1 (apoptotic) peak by FACS analysis. Western blotting results clearly indicated cleavage of Caspase-3 and PARP. Casp-glow assay confirmed activation of caspase-3, an important mediator of apoptosis. Semiquantitative RT-PCR analysis showed an up-regulation of pro-apoptotic genes (p21, p53 and Bax) and down-regulation of the anti-apoptotic Bcl-2 gene, which is an important hallmark of an apoptosis.

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Section: Caspase-3 Activity Assaysupporting

confidence: 82%

Induction of Caspase-3 Dependent Apoptosis, Cell Cycle Arrest and Cytotoxicity in Breast Cancer Cells by Abrus Precatorius

Sofi

Nabi

2018

Int J Pharm Pharm Sci

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“…In addition, some findings suggested that the antitumor activity of carvacrol is not due to direct cytotoxicity but possibly by prevention of prenylation of several proteins including Ras (18). Reports state that its mechanism is due to its antioxidant nature (19).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Effect of Thymus caramanicus Jalas on Human Oral Epidermoid Carcinoma KB Cells

et al. 2017

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Identifying new chemotherapeutic agents with fewer side effects is a major concern for scientists today. Thymus caramanicus Jalas (Lamiaceae family) is one of the species of Thymus that grows wild in different regions of Iran. Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis and cancer. Here was investigated the cytotoxic property of Thymus caramanicus essential oil and extract in human oral epidermoid carcinoma KB cells. Cell viability was measured by MTT and neutral red assays. The cells were exposed to different concentrations of essential oil (0.05-1 µL/mL) and extract (25-150 µg/mL) for 24 h. Doxorubicin was used as anticancer control drug. The data showed that the essential oil (IC50=0.44 µL/mL) and extract (IC50=105 µg/mL) induce potent cytotoxic property. Surprisingly, cytotoxic effects of essential oil and extract of this plant on KB cancer cells were greater than those on normal gingival HGF1-PI1 cell line. In addition, Thymus caramanicus could potentiate the effect of doxorubicin in subeffective concentrations. The results of the present study indicate that essential oils and extracts of Thymus caramanicus have potential anti-proliferative property on KB cells and can be used as pharmaceutical case study for oral cancer treatments.

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“…The assay was carried out according to previous studies. [15][16][17] Briefly, A549 and HeLa cells were cultured (2 Â 10 3 ) in 96 well plates and 0.1 to 50 mg ml À1 NBA derivatives were added. The plates were incubated for 24 and 48 h. After each time period, 20 ml (5 mg ml À1 ) MTT dye was added and the plates were measured with a ELx808-IU Bio-Tek apparatus at 540 nm.…”

Section: Mtt Assaymentioning

confidence: 99%

Antiproliferative effects of some N‐benzylideneanilines

Özkay

İncesu

Işıkdağ

et al. 2007

Cell Biochemistry & Function

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A series of Schiff bases including N-benzylideneaniline (NBA) nuclei were prepared. The chemical products obtained were characterized by mass spectometry (APCI), 1H NMR, and IR spectroscopy in order to seek their cytotoxic and proliferation effects on human small lung (A549) and cervical (HeLa) cancer cell lines with biochemical assays. All of the synthesized compounds showed antiproliferative effects to different extents.

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Inhibition of DNA synthesis by Carvacrol in mouse myoblast cells bearing a human N-RAS oncogene

Cited by 88 publications

References 24 publications

Induction of Caspase-3 Dependent Apoptosis, Cell Cycle Arrest and Cytotoxicity in Breast Cancer Cells by Abrus Precatorius

Induction of Caspase-3 Dependent Apoptosis, Cell Cycle Arrest and Cytotoxicity in Breast Cancer Cells by Abrus Precatorius

Cytotoxic Effect of Thymus caramanicus Jalas on Human Oral Epidermoid Carcinoma KB Cells

Antiproliferative effects of some N‐benzylideneanilines

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