Inhibition of dentine demineralization by zinc oxide: In vitro and in situ studies

Takatsuka, Tsutomu; Tanaka, Keiko; Iijima, Youichi

doi:10.1016/j.dental.2005.02.006

Cited by 56 publications

(45 citation statements)

References 15 publications

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“…It may also be that effective inhibitors of MMPs included in resin-dentine bonding agents may protect the seed crystallite-sparse collagen fibrils of the scaffold from degradation, permitting them to be remineralized [Liu et al, 2011]. Zinc has also been shown to inhibit dentine demineralization [Takatsuka et al, 2005] and facilitates dentine remineralization [Lynch et al, 2011]. These effects make zinc attractive for use as a therapeutic agent in the fields of hard and soft tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

et al. 2012

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Background: Dentine matrix metalloproteinases (MMPs) play an important role in the dentine caries process. Aims: To determine if MMP-mediated collagen degradation of acid-demineralized dentine may be inhibited by zinc or zinc chelators. Methods: Human dentine specimens were demineralized by phosphoric acid (PA), ethylenediaminetetraacetic acid (EDTA), Clearfil SE Bond primer (SE), or Xeno V (XE) and stored in artificial saliva. Chlorhexidine digluconate (CHX), doxycycline, EDTA, or ZnCl₂ was added. C-terminal telopeptide determinations (ICTP) were performed by radioimmunoassay after 24 h and 4 weeks. Results: Collagen degradation was prominent in PA-demineralized (ICTP values from 74.01 µg/l at 24 h to 202.46 µg/l after 4 weeks) and EDTA-demineralized dentine (ICTP values from 83.93 µg/l at 24 h to 158.82 µg/l after 4 weeks) stored in artificial saliva. Doxycycline fully blocked proteolysis. CHX and EDTA reduced collagen degradation only at 24 h. Zinc in excess strongly inhibited hydrolysis of collagen in all tested groups (ICTP values were: PA, 13.56 µg/l; EDTA, 11.21 µg/l; SE, 1.52 µg/l, and XE, 2.37 µg/l) and its effect was maintained for up to 4 weeks, except for EDTA-treated dentine (ICTP values were: PA, 40.76 µg/l; EDTA, 79.15 µg/l; SE, 5.29 µg/l, and XE, 6.38 µg/l). Conclusion: EDTA and CHX exerted time-limited MMP inhibition, and excess zinc served as an effective inhibitor of MMP-mediated collagen degradation in strong or mildly demineralized dentine. MMP degradation of collagen was reduced in resin-infiltrated dentine; the presence of excess zinc chloride exerted an additional protective effect.

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Section: Discussionmentioning

confidence: 99%

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

et al. 2012

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“…Zinc has been demonstrated to reduce MMPs-mediated collagen degradation (Osorio et al, 2011), to inhibit dentin demineralization (Takatsuka et al, 2005) and to induce dentin remineralization at the bonded interface (Toledano et al, 2013a). Zinc influences signaling pathways and stimulates a metabolic effect in hard tissue mineralization (Hoppe et al, 2011) and remineralization processes (Barcellos et al, 2016;Lynch et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

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“…Zinc has also been shown to inhibit dentin demineralization [Takatsuka et al, 2005] and somehow facilitates enamel remineralization [Lynch et al, 2011]. These effects make zinc attractive for use as a therapeutic agent in the fields of hard and soft tissue engineering.…”

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confidence: 99%

Zinc Induces Apatite and Scholzite Formation during Dentin Remineralization

Osorio

Cabello

et al. 2014

Caries Res

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The aim of this study was to ascertain whether zinc may improve the repair ability of demineralized dentin. Dentin disks were demineralized by phosphoric acid during 15 s and immersed in artificial saliva, remineralizing solution, a zinc chloride solution and a zinc oxide solution. Dentin specimens were analyzed after 24 h and 1 month of storage. Surface morphology was assessed by atomic force and scanning electron microscopy, mechanical properties were analyzed by nanohardness testing in a TriboIndenter, and chemical changes at the surfaces were determined by X-ray diffraction, Raman and energy-dispersive elemental analyses. After phosphoric acid application, dentin was only partially demineralized. Demineralized dentin was remineralized after 24 h of storage in any of the tested solutions (nanohardness increased and hydroxylapatite formation was detected by Raman). Remineralization was maintained up to 1 month in dentin stored in remineralizing solution, zinc chloride and zinc oxide. Zinc and phosphate were important for hydroxylapatite homeostasis. Scholzite formation was encountered in dentin stored in zinc-containing solutions. Zinc might allow to reach the balance between dentin demineralization and remineralization processes.

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Inhibition of dentine demineralization by zinc oxide: In vitro and in situ studies

Cited by 56 publications

References 15 publications

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

Zinc-Inhibited MMP-Mediated Collagen Degradation after Different Dentine Demineralization Procedures

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Zinc Induces Apatite and Scholzite Formation during Dentin Remineralization

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