Inhibition of Cuticle Deposition and Chitin Biosynthesis by a New Insect Growth Regulator, Buprofezin, inNilaparvata lugensStål

Mode of Action of Buprofezin on the Twenty-eight-spotted Ladybird,Henosepilachna vigintioctopunctataFabricius

“…Thus, the lethal effect of buprofezin on H. vigintioctopunctata larvae can be attributed to the inhibition of chitin biosynthesis, as already reported for N. lugens nymphs. 4 ,S) Indeed, the poisoning, symptoms of H. vigintioctopunctata larvae intoxicated with buprofezin were quite similar to those reported for other chitin biosynthesis inhibitors.…”

Challenges with managing insecticide resistance in agricultural pests, exemplisfied by the whitefly Bemisia tabaci

“…Although both chemicals act primarily against immature stages of white£ies, they possess distinct modes of action and are therefore very unlikely to be a¡ected by the same resistance mechanism. Buprofezin inhibits chitin synthesis and results in nymphal death during ecdysis (Uchida et al 1985), whereas pyriproxyfen is a juvenile hormone mimic interrupting nymphal and pupal development, but also suppressing egg hatch by direct exposure of eggs or transovarially via the treatment of adult females (Ishaaya & Horowitz 1992). These di¡erences, coupled with a high degree of species selectivity, make them ideally suited as rotation partners in control programmes that place emphasis on the preservation of natural enemies of B. tabaci and co-existing pest species .…”

Ultrastructural changes in the cuticle of the greenhouse whitefly Trialeurodes vaporariorum, induced by the insect growth inhibitor, buprofezin

“…Uchida et al (1985) reported, after observation by means of the light microscope, that the pharate cuticle ofbuprofezin treated N. lugens nymphs did not stain with Mallory triple stain after 84 h, in contrast with that of untreated ones. Uchida et al (1985) reported, after observation by means of the light microscope, that the pharate cuticle ofbuprofezin treated N. lugens nymphs did not stain with Mallory triple stain after 84 h, in contrast with that of untreated ones.…”

“…To our knowledge, no data concerning the effect of buprofezin on the ultrastructure of the integument have been published until now. Uchida et al (1985) reported, after observation by means of the light microscope, that the pharate cuticle ofbuprofezin treated N. lugens nymphs did not stain with Mallory triple stain after 84 h, in contrast with that of untreated ones. Suppression of normal cuticle development was observed in Lucilia cuprina (Binnington, 1985), in Leptinotarsa decemlineata (Hegazy et al, 1989) and in Musca 301 domestica (Degheele et al, 1988) after treatment with diflubenzuron or other chitin synthesis inhibitors.…”