Inhibition of Chlamydia trachomatis growth in McCoy, HeLa, and human prostate cells by zinc

Greenberg, Stephen B.; Harris, Dominic A.; Giles, Petrina B.; Martin, Rachel W.; Wallace, R. J.

doi:10.1128/aac.27.6.953

Cited by 18 publications

(9 citation statements)

References 27 publications

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“…The antimicrobial spectrum of Zn has been suggested to include many potential genitourinary pathogens, gram‐positive and gram‐negative ones (Fair et al. , 1976; Greenberg et al. , 1985).…”

Section: Introductionmentioning

confidence: 99%

Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury

2009

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The relationship of calcium, magnesium, zinc (Zn) and copper (Cu) concentrations in blood and seminal plasma on sperm parameters of spinal cord-injured (SCI) men and of normal controls was determined. The study included 93 SCI men and 145 neurologically intact men. Colorimetric and flame atomic absorption spectrophotometers were used. Seminal Zc and Cu had a relationship with sperm motility in SCI men.

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Section: Introductionmentioning

confidence: 99%

Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury

2009

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“…Zinc ions inhibit the in vitro replication of diverse viruses, including rhinovirus (4,5), herpes simplex virus (7), and vaccinia virus (3), and other pathogenic organisms, including Chlamydia trachomatis (2). Zinc chloride (0.1 mM) inhibited plaque formation by eight of nine human rhinoviruses in HeLa cells and reduced the yield of rhinovirus 1A when added as late as 6 to 8 h after infection (5).…”

mentioning

confidence: 99%

In vitro activity of zinc salts against human rhinoviruses

Geist¹,

Bateman²,

Hayden³

1987

Antimicrob Agents Chemother

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The antiviral activity of zinc salts against rhinovirus types 1A and 39 was assayed by yield reduction and inhibition of cytopathic effect in cell culture. The findings indicate that the zinc salts tested have low in vitro therapeutic indices and suggest that the possible beneficial effects of zinc lozenges in reducing cold symptoms may not be related to selective antirhinovirus activity.Zinc ions inhibit the in vitro replication of diverse viruses, including rhinovirus (4, 5), herpes simplex virus (7), and vaccinia virus (3), and other pathogenic organisms, including Chlamydia trachomatis (2). Zinc chloride (0.1 mM) inhibited plaque formation by eight of nine human rhinoviruses in HeLa cells and reduced the yield of rhinovirus 1A when added as late as 6 to 8 h after infection (5). The antirhinovirus activity of this zinc salt appears to be mediated by inhibition of posttranslational cleavage of precursor polypeptides (4). Zinc gluconate lozenges were reported to be effective in reducing the duration of symptoms in natural colds of undefined viral etiology (1). In contrast, another study, using a somewhat lower dosage of zinc acetate lozenges, found no clinical benefit compared with a placebo in the treatment of natural colds (R. Douglas, personal communication). Unpalatable taste, mouth irritation, and nausea have been reported side effects of zinc lozenge administration (1; B. M. Farr and J. M. Gwaltney, Jr., J. Chronic Dis., in press).The present studies were undertaken to determine the in vitro antiviral activity of a variety of zinc salts against two human rhinoviruses representing serotypes previously shown to be inhibited by zinc chloride (5). Serotype 1A (passage history: MRC-5/5, WI-38/1, fetal tonsil/1) and serotype 39 (passage history: WI-38/2, MRC-5/4) were used. The following zinc salts (molecular weight) were provided by R. G. Achari, Bristol Meyers Products, Inc., Hillside, N.J.: acetate (243.5), carbonate (125.4), chloride (136.3), gluconate (455.7), lactate (183.5), sulfate (161.4), and oxide (81.4). The salts were dissolved in sterile distilled water or 0.1 N HCl to make stock solutions of 10 mM concentration. Serial logl0 or 0.5 log10 dilutions of the stock solutions were made in cell culture maintenance medium appropriate to the cell type. MRC-5 and WI-38 human embryonic lung fibroblast cells (M.A. Bioproducts, Inc., Walkersville, Md.) at passage levels 23 to 25 were maintained in Eagle minimal essential medium supplemented with glutamine, 2% fetal bovine serum (HyClone Laboratories, Logan, Utah), vancomycin (20 jig/ml), gentamicin (50 ,ug/ml), and amphotericin (1 jig/ml). For cytopathic effect (CPE) inhibition and infectious virus yield reduction assays, monolayers of a particular cell type were inoculated with 0.2 ml of Hanks balanced salt solution (HBSS) containing 30 to 1,000 50% tissue culture infective doses (TCID50) of virus. After incubation at 35°C for 1 h, the inoculum was decanted, and the monolayers were rinsed once or twice with Hanks balanced salt solution. The monolayers were o...

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“…Zinc salts have been shown to have in vitro activity against a variety of pathogens, including the causative agents of a number of important sexually transmitted diseases (STD), such as human immunodeficiency virus, herpes simplex virus (HSV), and Chlamydia trachomatis (1,9,11,12,14,17,18). In addition to in vitro activity, mice inoculated intravaginally with HSV type 2 (HSV-2) and then treated either with 100 mM zincmedicated collagen sponges or by an intravaginal 100 mM zinc salt douche had reduced incidences of genital herpes compared to controls (19,20).…”

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confidence: 99%

Efficacy and Toxicity of Zinc Salts as Candidate Topical Microbicides against Vaginal Herpes Simplex Virus Type 2 Infection

Bourne

Stegall

Montano

et al. 2005

Antimicrob Agents Chemother

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Zinc salt solutions administered as topical microbicides provided significant protection against herpes simplex virus type 2 infection in a mouse vaginal challenge model. However, at the therapeutic concentration, the salt solutions caused sloughing of sheets of vaginal epithelial cells. These observations limit the utility of zinc salts as microbicides and suggest that the application of zinc solutions to mucosal surfaces has the potential to cause damage that might increase susceptibility to secondary infections at a later time.

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Inhibition of Chlamydia trachomatis growth in McCoy, HeLa, and human prostate cells by zinc

Cited by 18 publications

References 27 publications

Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury

Concentration of blood and seminal plasma elements and their relationships with semen parameters in men with spinal cord injury

In vitro activity of zinc salts against human rhinoviruses

Efficacy and Toxicity of Zinc Salts as Candidate Topical Microbicides against Vaginal Herpes Simplex Virus Type 2 Infection

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