Inhibition of cell proliferation on lens capsules by 4197X-ricin A immunoconjugate

Abstract

Immunotoxin 4197X-RA may help prevent PCO after primary cataract surgery.

Specific features of apoptosis in human lens epithelial cells induced by mitomycin C in vitro

“…], certain IOL materials and sharp-edged IOL designs ("contact inhibition"). Different pharmacological studies have been conducted, using immunotoxins and cytotoxins [21], lens epithelial necrosis factor (LENF) [8], proteolytic enzymes [17] or a sustained drug delivery system (SDDS) [22]. Antimetabolic strategies have been investigated in vitro and in vivo, using mainly daunomycin (also called daunorubicin) [6,23] or mitomycin C (MMC) [7,25].…”

Selective Death of Lens Epithelial Cells Using Demineralized Water and Triton X-100 With PerfectCapsule Sealed Capsule Irrigation

“…13 To target LECs, a number of cytotoxic agents have been investigated previously in human and animal studies, ex vivocapsularbagorganculture,andLECculturesinvitro. [14][15][16][17][18][19][20][21][22][23][24][25][26] Although the aim was to selectively target the LECs, the researchers were unable to avoid toxic effects to other intraocular tissues, such as the sensitive corneal endothelium, which was a concern while using these agents. Selective targeting of LECs to prevent PCO can now be safely conducted using sealed capsule irrigation (SCI) by precise delivery of known doses of pharmacologic agents to eliminate the risk of toxic side effects to surrounding intraocular structures.…”

“…(eg, antimetabolites such as methotrexate, mitomycin, daunomycin, 5-fluorouracil, colchicine, and daunorubicin), anti-inflammatory substances, hypo-osmolar drugs, and immunoconjugate (MDX-RA; Medarex Inc, Princeton, NJ) and immunological agents. [14][15][16][17][18][19][20][21][22][23][24][25] Immune targeting has been investigated using ricin A immunotoxin 26 with good effect, but it is complicated by suspected collateral damage to the retina, possibly due to a crossreaction of the antibody with retinal epithelium. The specific delivery of agents to LECs has been difficult.…”

Evaluation of a human capsular bag model for secondary cataract determination after intraocular lens implantation

“…In vitro studies using tissue-cultured LECs cannot provide these results; they can easily test the inhibitory effects of different substances [7,18], but not the effect of IOL design within the physiological system of the capsular bag. Animal model systems cannot consider speciesdependent factors [4,20].…”