Inhibition of blood clearance and hepatic tissue binding of Escherichia coli by liver lectin-specific sugars and glycoproteins

Abstract

The effects of sugars and glycoproteins that are known to bind to lectins of liver tissue on the clearance of cells of Escherichia coli from mouse blood was investigated. The administration of 100 mg per mouse of methyl-at-D-mannoside, methyl-a-D-glucoside, or methyl-cx-D-fucoside, but not of methyl-a-D-galactoside or L-rhamnose, markedly inhibited the blood clearance of cells of E. coli 346. Clearance was similarly inhibited by 0.1 and 1.0 mg per mouse of asialofetuin or ovalbumin, respectively, whereas fetuin had no effect. The inhibitory effects of the sugars on blood clearance was abolished by pretreating the E. coli cells with antibodies against whole organisms. All of these effects were equal for fimbriated and nonfimbriated phenotypes of E. coli 346. Homogenates of mouse liver tissue coaggregated with nonfimbriated cells of E. coli. The aggregation was blocked by 100 mM solutions of methyl-a-D-mannoside, or methyl-oa-D-glucoside, 1 mg of bacterial lipopolysaccharide per ml, or 10 mM EDTA but not by L-rhamnose. These results suggest that the mannose-N-acetylglucosamine hepatic lectin recognizes specific sugars on the surface of E. coli and may be centrally involved in the nonimmune clearance of nonfimbriated E. coli from the blood of the infected host.255:4607-4613. 7. Firon, N., I. Ofek, and N. Sharon. 1983. Carbohydrate specificity of the surface lectins of Escherichia coli, Klebsiella pneumo-

Effects of Inactivated Enterococcus faecalis and Mannan Oligosaccharide and Their Combination on Growth, Immunity, and Disease Protection in Rainbow Trout

“…Mannose-specific lectins belong to this group of PAMP molecules. They function not only as adhesins, binding bacteria like Campylobacter jejuni (McSweegan and Walker 1986) and A. hydrophila (Merino et al 1996) to epithelial cells, but also as stimulators of phagocytic cell activities (Perry and Ofek 1984;Wright et al 1989).…”

An Elevated Dietary Level of Ascorbic Acid Fails to Influence the Response of Anterior Kidney Neutrophils toEdwardsiella ictaluriin Channel Catfish

“…Bactericidal-static assay. -Killing of E. ictaluri by neutrophils for each fish was assessed according to a plate-count method to determine the number of colony-forming units (CFU) of unphagocytized bacteria (Perry and Ofek 1984;Honda et al 1986). At 0, 90, 180, 270, and 360 min, a 0.1-mL aliquot was diluted in 0.9 mL sterile distilled water to release engulfed and all adsorbed bacteria.…”

Neutrophil–Kupffer-cell interaction in host defenses to systemic infections

“…The reduced capacity of mice pretreated i.v. with sugars (e.g., methyl-␣-D-mannoside) or neoglycoproteins (mannose-conjugated bovine serum albumin) to remove organisms from the blood suggests that clearance is mediated in part by the interaction of lectins expressed by Kupffer cells and carbohydrate residues expressed by the bacteria [48,49]. Indeed, fixed tissue macrophages exhibit receptors specific for ligands present on the surface of microorganisms, but not normally displayed by host cells.…”