An Elevated Dietary Level of Ascorbic Acid Fails to Influence the Response of Anterior Kidney Neutrophils to<i>Edwardsiella ictaluri</i>in Channel Catfish

Johnson, Michael R.; Ainsworth, A. Jerald

doi:10.1577/1548-8667(1991)003<0266:aedloa>2.3.co;2

Cited by 17 publications

(10 citation statements)

References 26 publications

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“…It has been hypothesized that high levels of vitamin C might inhibit cortisol biosynthesis by preventing the conversion of unsatured fatty acids into cholesterol ester which is the precursor of the hormone molecule (Montero et al, 1999). However, studies suggest that post-stress cortisol concentration is not dependent on vitamin C (Johnson & Ainsworth, 1991, Li et al, 1998Ortuño et al, 2003) as observed in our study. According to Dabrowska et al (1991) the relationship between plasma cortisol and tissue concentration of ascorbate is not known in fish, and questions relating to ascorbic acid status and steroidogenesis are still unclear.…”

contrasting

confidence: 63%

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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The role of vitamin C on physiological responses of matrinxã (Brycon amazonicus) submitted to air exposure was analyzed. Nine hundred fish (70.15 g) were distributed in fifteen 500 l boxes (60 fish.box -1 ) and fed five rations (treatments): Control (no vitamin C); T100 (100 mg); T200 (200 mg); T400 (400 mg) and T800 (800 mg of vitamin C kg.ration -1 ). Each ration was offered to fish of three boxes during 60 days before the stress challenge that consisted of exposing fish to air for two minutes. Samplings were carried out for 5, 15, 30 and 60 minutes after the air exposure. Blood was collected for glucose, cortisol, total protein, sodium, chloride, hematocrit, hemoglobin determination, and white and red cell count. Liver was removed for hepatosomatic index (HSI) calculation and glycogen determination. Vitamin C did not affect the levels of cortisol, chloride, total protein, hemoglobin, leukocytes, hepatic glycogen or HSI in air exposed fish. Blood glucose levels elevation observed 60 minutes after the challenge did not depend on the levels of vitamin C, nor did the drop in serum sodium levels verified 60 minutes after stressor. In general, hematocrit did not change by effect of vitamin C but it was lower at 15 and 30 minutes after the challenge. The number of erythrocytes decreased in fish after 5 minute sampling in all treatments, especially at 30 and 60 minutes. The air exposure evoked alterations in stress indicators of matrinxã, and the vitamin C did not alter the responses. KEY WORDSBrycon amazonicus, capture, stress, ascorbyl -polyphosphate. de glicose, cortisol, proteína total, sódio, cloreto, hematócrito, hemoglobina, contagem Respostas fisiológicas de matrinxã (Brycon amazonicus) arraçoados com diferentes níveis de vitamina C e submetidos à exposição aérea RESUMO Este trabalho avaliou a participação da vitamina C nos indicadoras de estresse no matrinxã (Brycon amazonicus), durante exposição aérea. Novecentos peixes (70,15g) foram distribuídos em caixas de cimento (500 l) e alimentados com os tratamentos: Controle (sem vitamina C); T100 (100 mg); T200 (200 mg); T400 (400 mg); T800 (800 mg de vitamina C kg.ração -1 ). Cada ração foi fornecida a peixes de três caixas por 60 dias antes da aplicação do estressor, que consistiu em suspender os peixes mantendo-os no ar por dois minutos. Cinco, 15, 30 e 60 minutos depois, os peixes anestesiados foram amostrados. Foi coletado sangue para determinação PALAVRAS -CHAVEBrycon amazonicus, captura, estresse, ascorbil polifosfato.

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contrasting

confidence: 63%

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Abreu

Urbinati

2006

Acta Amaz.

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“…Vitamin C and vitamin E, singly or in combinations are the most extensively studied vitamins for their influence on immunity and disease resistance in fish (Blazer and Wolke 1984; Li and Lovell 1985; Johnson and Ainsworth 1991; Blazer 1992; Hardie et al 1993; Waagbø et al 1993; Wise et al 1993; Verlhac and Gabaudan 1994; Li et al 1998; Mulero et al 1998; Walhi et al 1998; Ortuno et al 1999; Ortuno et al 2001; Sealey and Gatlin 2002; Lim et al 2006). Dietary deficiencies of vitamins C and E have been shown to decrease immune response and increase susceptibility to infection.…”

Section: Discussionmentioning

confidence: 99%

Growth Performance, Immune Response, and Resistance toStreptococcus iniaeof Nile Tilapia,Oreochromis niloticus, Fed Diets Containing Various Levels of Vitamins C and E

Lim¹,

Yildirim‐Aksoy²,

Welker³

et al. 2010

Journal of the World Aquaculture Society

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Growth, immunity, and resistance of Nile tilapia to Streptococcus iniae challenge were evaluated after feeding diets supplemented with vitamin C (0, 100, 2000 mg/kg) and E (0, 50, 500 mg/kg) for 12 wk. Supplementation of 100 mg vitamin C/kg to the basal diet was sufficient to increase growth and feed efficiency. The amount of vitamin E present in the basal diet (23.1 mg/kg) was sufficient to promote good growth and feed efficiency, but adding 50 mg vitamin E/kg was necessary to increase survival. Liver vitamin C and E concentrations increased with increasing dietary concentrations of the corresponding vitamin. Dietary vitamin E concentrations had no effect on liver vitamin C concentration, but increasing dietary vitamin C increased liver vitamin E. Although hematology was generally unaffected by dietary vitamin E, significantly lower red blood cell count and hemoglobin and higher mean corpuscular volume were observed in fish fed the vitamin C‐unsupplemented diets. Total immunoglobulin and lysozyme activity were significantly higher and lower, respectively, in fish fed 2000 mg/kg vitamin C diets. Vitamin E at 500 mg/kg diet significantly decreased alternative complement activity. Dietary concentrations of vitamin C had no effect on mortality following S. iniae challenge, but mortality significantly decreased in fish fed vitamin E–supplemented diets.

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“…Because of the very rapid rate of growth during this early stage (fry) in life, a higher dose of vitamin C was given to C. mrigala . However, bigger fish require lower levels of vitamin C than that required by smaller ones (Johnson & Ainsworth 1991). Thus, the dose of vitamin C was maintained up to 500 mg kg −1 practical feed during feeding to fingerlings of L. rohita .…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary vitamin C on immunity, growth and survival of Indian major carp Labeo rohita, fingerlings

Misra¹,

Das²,

Mukherjee³

et al. 2007

Aquaculture Nutrition

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An 8‐week feeding trial was conducted to evaluate the efficacy of dietary l‐ascorbic acid (AA) dosages on immunity, growth and survival of the fingerlings of Labeo rohita. AA was supplemented at 0, 100, 200 and 500 mg kg−1 basal diets. Each diet was fed for 8 weeks to duplicate groups of fingerlings of rohu with initial body weight of 35 ± 5 g. Selected biochemical and haematological analyses were evaluated every second week. At the end of the feeding trial of 56 days, fish were divided into two subgroups under each major treatment group for challenge with two pathogens, Aeromonas hydrophila and Edwardsiella tarda. The mortality (%) and agglutinating antibody titre were recorded on 28th day postchallenge. Non‐specific immune parameters like superoxide anion production, lysozyme activity and complement activity increased independently of the levels of AA in the diet. Whereas phagocytic ratio, phagocytic indices, lymphokine production indices and serum bactericidal activity were better in the group of fish fed with 200 mg AA kg−1 feed for 42 days. However, growth parameters such as specific growth rate, feed conversion ratio and survival were better in the group of fish fed with the highest level of AA through feed for 56 days. These data indicated that elevated levels of dietary AA could be recommended for optimum immunity, growth and survival of fingerlings of L. rohita.

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An Elevated Dietary Level of Ascorbic Acid Fails to Influence the Response of Anterior Kidney Neutrophils toEdwardsiella ictaluriin Channel Catfish

Cited by 17 publications

References 26 publications

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Physiological responses of matrinxã (Brycon amazonicus) fed different levels of vitamin C and submitted to air exposure

Growth Performance, Immune Response, and Resistance toStreptococcus iniaeof Nile Tilapia,Oreochromis niloticus, Fed Diets Containing Various Levels of Vitamins C and E

Effects of dietary vitamin C on immunity, growth and survival of Indian major carp Labeo rohita, fingerlings

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