Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone

Ren, Dacheng; Sims, James J.; Wood, Thomas K.

doi:10.1046/j.1472-765x.2002.01087.x

Cited by 129 publications

(94 citation statements)

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“…[30][31][32][33][34][35][36]55 Furanone-1 [(5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone] has been shown to inhibit >70% of the AI-2 regulated genes in E. coli, has been observed in Streptococcus pyogenes. 50 In total, these data demonstrate that the altered phenotypes in the ∆luxS strain are due to luxS disruption and not to other undefined coincident mutations.…”

Section: Discussionmentioning

confidence: 99%

“…Inhibition of QS circuits by QS antagonists, such as the halogenated furanones from the redsea alga Delisea pulchra, offers an attractive method for inhibiting bacterial pathogens. [26][27][28][29][30][31][32][33][34][35][36] B. anthracis synthesizes AI-2 or an AI-2-like auto-inducer molecule that induces bioluminescence in the Vibrio harveyi Quorum-sensing (Qs), the regulation of bacterial gene expression in response to changes in cell density, involves pathways that synthesize signaling molecules (auto-inducers). The luxS/AI-2-mediated QS system has been identified in both Grampositive and Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Jones¹,

Peterson²,

Benn³

et al. 2010

Virulence

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Jones¹,

Peterson²,

Benn³

et al. 2010

Virulence

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“…Negative and positive controls were sterile CFM and CFM from highdensity cultures of 34F 2 ⌬luxS and V. harveyi, respectively. synthetic furanone, (5Z)-4-bromo-5-(bromethylene)-3-butyl-2(5H)-furanone, has the ability to inhibit AI-2-mediated quorum sensing in E. coli and V. harveyi (19) as well as swarming and biofilm formation by B. subtilis (20). Examination of this or similar molecules could permit ascertainment of the role of AI-2-mediated mechanisms in virulence gene expression of B. anthracis.…”

Section: Discussionmentioning

confidence: 99%

Detection of a luxS -Signaling Molecule in Bacillus anthracis

Jones

2003

Infect Immun

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Quorum-sensing regulation of density-dependent genes has been described for numerous bacterial species. The partially annotated genome sequence of Bacillus anthracis contains an open reading frame (BA5047) predicted to encode an ortholog of luxS, required for synthesis of the quorum-sensing signaling molecule autoinducer-2 (AI-2). To determine whether B. anthracis produces AI-2, the Vibrio harveyi luminescence bioassay was used. Cell-free conditioned media from vaccine (Sterne) strain 34F 2 induced luminescence in V. harveyi reporter strain BB170, indicating its production of AI-2. Cloned BA5047, expressed in Escherichia coli DH5␣ cells, restored AI-2 activity to these cells. To evaluate whether BA5047 is essential for AI-2 synthesis, it was deleted through allelic exchange with marker rescue; the resulting mutant had no functional luxS activity and had reduced growth in vitro. In the wild-type strain, AI-2 activity was greatest during the exponential phase of growth. In total, these data indicate that BA5047 is a functional luxS ortholog in B. anthracis necessary for growth-phase-specific AI-2 expression. Thus, B. anthracis may utilize extracellular signaling molecules to regulate density-dependent gene expression.Quorum sensing is the regulation of bacterial gene expression in response to changes in cell density (13). Bacteria that utilize quorum-sensing signaling pathways synthesize signaling molecules (autoinducers [AI]) which have been found in nature as N-homoserine lactones (8) or small peptides (13). AI levels are directly proportional to the size of the bacterial population (7), and at threshold levels detectable by bacterial cell receptors, AI binding alters bacterial gene expression (13). Quorum-sensing-based regulation of gene expression is critical for the pathogenesis of clinically important bacterial infections, such as those due to Pseudomonas aeruginosa in patients with cystic fibrosis (4) or to Vibrio cholerae (14).Quorum sensing has been well characterized in Vibrio harveyi, a bioluminescent bacterium that freely lives in the ocean floor sediment or on the exterior of fish (17). The luminescence genes are expressed only when the V. harveyi populations are at high cell density under the control of the lux quorumsensing system (13). The luxCDABE operon is regulated by two-component systems that are stimulated by the AI ligands, AI-1 (acyl-homoserine lactone [AHL]) and AI-2 (13). Synthesis of AI-1 requires luxLM. AI-1 diffuses freely through the cell wall into the extracellular milieu, and when sufficient quantities are recognized by its sensor histidine kinase, luxN, a hybrid two-component system-signaling cascade is initiated (13). The lux cascade also is regulated by another AI molecule, AI-2, which is predicted to be a furanosyl borate diester and is synthesized by the product of luxS (2). The luxS product converts S-ribosylhomocysteine to 4,5-dihydroxyl-2,3-pentanedione, catalyzing AI-2 formation (2). V. harveyi strain BB170, in which luxN is mutated, is unable to detect AI-1 molecules and m...

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“…Epidural and subarachnoid block were used successfully in patients for non-carcinoid surgery [27,28]. But care must be taken for cardiovascular stability as these procedures can cause hypotension which can be resistant to the usual therapy as sympathomimetics can exacerbate the condition.…”

Section: Regional Anesthesiamentioning

confidence: 99%

Perioperative Management of Carcinoid Syndorme-An Anaesthesiologist’s Perspective

Mvs¹

2016

J Anesth Clin Res

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Occurrence of carcinoid syndrome is very rare. So the expertise and literature on this syndrome is limited. Physiologically active substances that are secreted by the carcinoid tumour cause major problems for the anesthesiologist in the perioperative period. Preoperative preparation of the patient is the most important step that decides the recovery of the patient. Discovery of octreotide lead to the improvement in treating and decreasing the morbidity and mortality during surgery. If the surgery is palliative post-operative management also makes it difficult for the anesthesiologist. Extreme vigilance and common sense had to be applied during the surgery by the anesthesiologist. Pathophysiology, symptomatology, diagnosis and perioperative management are described in this article.

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Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone

Cited by 129 publications

References 31 publications

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Detection of a luxS -Signaling Molecule in Bacillus anthracis

Perioperative Management of Carcinoid Syndorme-An Anaesthesiologist’s Perspective

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