Role ofluxSinBacillus anthracisgrowth and virulence factor expression

Disrupting theluxSquorum sensing gene does not significantly affectBacillus anthracisvirulence in mice or guinea pigs

“…A previous report indicated that a luxS mutation in the attenuated Sterne strain of B. anthracis led to decreased growth in liquid culture 13 . Given this relationship between LuxS activity and bacterial growth, we compared the growth kinetics of the Ames wild-type and luxS mutant strains in brain heart infusion (BHI) broth.…”

“…Researchers found subsequently that AI-2 QS inhibitors limit growth and toxin gene expression in the bacterium 9 . Recently, a luxS -deficient mutant of the B. anthracis Sterne strain was shown to exhibit similar phenotypic defects 13 . These findings suggest that opportunities might exist to treat B. anthracis infections using QS inhibitors.…”

LuxS contributes to virulence in avian pathogenic Escherichia coli O78:K80:H9

“…3). LuxS QS system has been shown in modulating the virulence gene expression (Jones et al, 2010;Sircili et al, 2004;Sperandio et al, 1999;Winzer et al, 2002;Zhu et al, 2002), bacterial growth (Jones et al, 2010), biofilm formation (Balestrino et al,Fig. 3.…”

“…In most cases, AI-2 has been implicated in controlling bacterial virulence. For example, LuxS is known to regulate the expression of a number of virulence genes involved in the pathogenesis of bacteria like enterohemorrhagic E. coli (EHEC) (Sperandio et al, 1999), enteropathogenic E. coli (EPEC) (Sircili et al, 2004;Sperandio et al, 1999), Neisseria meningitidis (Winzer et al, 2002), Vibrio cholerae (Zhu et al, 2002), Bacillus anthracis (Jones et al, 2010) or affecting biological behaviors such as B. anthracis bacterial growth (Jones et al, 2010), Klebsiella pneumoniae and Streptococcus pneumoniae biofilm formation (Balestrino et al, 2005;Vidal et al, 2011) and V. cholerae motility (Zhu et al, 2002). Although many studies have been carried out in the above-mentioned bacteria, the association between the luxS gene and the virulence of APEC has not been determined.…”

A Chemical Biology Approach to Interrogate Quorum-Sensing Regulated Behaviors at the Molecular and Cellular Level

“…In an analogous vein, the brominated furanone QS inhibitor, (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone, was utilized to study cell density-dependent processes in Bacillus anthracis . (Jones, et al, 2005; Jones, et al, 2010) Nevertheless, this method may also give rise to “red herrings” in that certain QS inhibitors may also interfere with essential bacterial processes and thus affect cell viability. (Lowery, et al, 2009) For example, the brominated furanones inhibit the biofilm formation of S. typhimurium , a commonly QS-associated process, but do so independently of the known Salmonella QS system.…”