Inhibition of Antigen-Antibody Reactions by Aminocarboxylic Acids

Atchley, William A.; Bhagavan, N.V.

doi:10.1126/science.138.3539.528

Cited by 21 publications

(6 citation statements)

References 3 publications

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“…Our results with lysine (a,e-diaminocaproic acid), for example, are similar to those of Atchley and Bhagaban [1] who found that various protein-antiprotein reactions were inhibited by aliphatic aminocarboxylic acids, with maximum inhibitory power residing in the compounds having a six-carbon chain and an amino group in the terminal position, thus e-aminocaproic acid was their best inhibitor for the precipitin reaction of crystalline bovine serum albumin with rabbit antiserum and for antiglobulin acting on globu lin-coated nuclei of leukocytes. Gill et al [6] found that, of the amino acids they tested, only lysine and arginine would inhibit the precipi tation of synthetic polypeptides and their antibodies.…”

Section: Effect Of Inhibitors On Uptake Of Nonspecific Sieva Lima Beasupporting

confidence: 78%

Inhibition of Hemagglutinins by Amino Acids

Chattoraj¹,

Boyd²

1965

Vox Sanguinis

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Section: Effect Of Inhibitors On Uptake Of Nonspecific Sieva Lima Beasupporting

confidence: 78%

Inhibition of Hemagglutinins by Amino Acids

Chattoraj¹,

Boyd²

1965

Vox Sanguinis

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“…In general, most other purines and purine derivatives were moderately good inhibitors whereas pyrimidines were relatively poor inhibitors. Although it has been reported that high concentrations of e-aminocaproic acid are capable of inhibiting antigenantibody reactions (18), no such effect was observed with the concentrations used in these experiments (Table I).…”

Section: Resultscontrasting

confidence: 53%

A Study of the Cross-Reactivity of Antipurin-6-Oyl Serum With Deoxyribonucleic Acid (Dna)

Butler

Tanenbaum

Beiser

1965

The Journal of Experimental Medicine

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The specificity of the reaction of antipurin-6-oyl sera with thermally denatured DNA has been studied by means of hapten inhibition techniques. The relative order of effectiveness of various haptens as inhibitors of the complement fixation reaction between DNA and antipurin-6-oyl serum was found to be comparable to their relative order of effectiveness as inhibitors of the precipitin reaction between purin-6-oyl-protein conjugates and antipurin-6-oyl serum. Antipurin-6-oyl antibody has been purified and has been shown to be capable of reacting with thermally denatured DNA. It is concluded that the reactivity of these purine-specific antisera with DNA is a property of antibody with specificity for the purin-6-oyl moiety. In addition, the reaction between antipurinoyl sera and DNA has been demonstrated by the techniques of radioimmunoelectrophoresis and passive cutaneous anaphylaxis.

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“…From these observations, it has been suggested that EACA does not inhibit the formation of antibodies, as cortisone , but rather has an effect on the antigen-antibody reaction. While the exact mode of the action is uncertain, inhibition of reactions between antigen and antibody may be due to specific relationships between the amino acid and the antigen-antibody complex (Atchley and Bhagavan, 1962). No effect of EACA was observed on the assay response to LATS .…”

Section: Immunofluorescent Localization Of Latsmentioning

confidence: 93%

“…EACA is known as an inhibitor of plasminogen activation, but also inhibits tissue reactions suspected to be allergic (tuberculin reaction) (Itoga and Yogo, 1959), immunohemolysis (Taylor and Fudenberg, 1964;Marschke and Tewksbury, 1966) , precipitation of antigen-antibody (Atchley and Bhagavan, 1962;Auerswald and Doleschel, 1966), homograft rejection (Gillette et al, 1963;Bertelli et al, 1964), and anaphylaxic reactions (Zweifach et al, 1961). Also, Atchley and Bhagavan reported an inhibitory effect of EACA on the reaction of globulincoated nuclei with fluorescent anti-globulin conjugates.…”

Section: Immunofluorescent Localization Of Latsmentioning

confidence: 99%

Comparative Studies on the Properties of LATS and TSH

Ochi

DeGroot

1970

Endocrinol Japon

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LATS activity was neutralized by pretreatment of mice with anti-IgG in vivo. Simultaneous injection of epsilon-amino caproic acid and hydrocortisone does not inhibit LATS action. Human thyroid-microsomal fractions neutralize LATS activity and TSH activity. The decrease in activity of LATS and TSH caused by thyroid microsomes correlates positively. Calf thyroid microsomes are much less active than human thyroid microsomes in absorbing LATS activity. Deoxycholate and sonification solubilize the LATS neutralizing activity. No visible precipitate formed between these solubilized fraction and LATS-IgG, using the Ouchterlony agar diffusion method. LATS did not differ from TSH in heat stability, and partially heat denatured LATS still had a long acting type response. Dissociation of a small rapidly acting molecule from LATS could not be obtained in high molar NaCI solution. LATS-IgG fragments digested by proteolytic enzymes (papain, pepsin, trypsin, and ficin) had a prolonged response like LATS. However, these fragments, in the presence of cysteine, either gave the same response after 2 hr and 8 hr, or gave a short acting response like TSH. The thyroid stimulating active site of the LATS active gamma globulin may be situated in Fab or Fab fragments having the antibody combining site. LATS did not stimulate the chicken thyroid, although TSH had this action. The proteolytic fractions of LATS-IgG also did not stimulate the chicken thyroid. No increase of gammaglobulin and/or IgG was found in some sera with a high LATS activity. Binding of LATS-IgG to mouse or human thyroid could not be visualized using the Coon's immunofluorescent method. These data support the concept that LATS is an IgG having a high specific activity of thyroid stimulating action.

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Inhibition of Antigen-Antibody Reactions by Aminocarboxylic Acids

Abstract: Aliphatic aminocarboxylic acids inhibit at least two different antigen-antibody reactions. The degree of inhibition depends upon carbon chain length and the position of the amino group in relation to the carboxyl group.

Cited by 21 publications

References 3 publications

Inhibition of Hemagglutinins by Amino Acids

Inhibition of Hemagglutinins by Amino Acids

A Study of the Cross-Reactivity of Antipurin-6-Oyl Serum With Deoxyribonucleic Acid (Dna)

Comparative Studies on the Properties of LATS and TSH

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