Inhibition of adenovirus 5 replication in COS-1 cells by antisense RNAs against the viral E1a region

Miroshnichenko, Olga I.; Ponomareva, T. I.; Tikchonenko, T.I.

doi:10.1016/0378-1119(89)90142-x

Cited by 14 publications

(2 citation statements)

References 27 publications

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“…Interference with viral functions by endogenously expressed AS mRNAs has already been achieved by using constitutively expressed specific AS transcripts, which are controlled by promoters that are activated by cellular transactivator factors and therefore expected to be expressed continuously. This approach has generally resulted in unsatisfactory degrees of in-hibition of viral replication, both for human immunodeficiency virus (4,17,23,32,37,38,39) and other viruses such as adenovirus (25,26), Moloney murine leukemia virus (43), human T-cell leukemia virus (44), and herpes simplex virus (HSV) (46). Both AS oligonucleotides (1) and transient expression of AS RNA oligomers (3), directed against immediate-early (IE) transcripts, have been shown to efficiently inhibit replication of HCMV.…”

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confidence: 99%

Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene

Ripalti

Boccuni

Campanini

et al. 1995

J Virol

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We have used an antisense RNA approach in the analysis of gene function in human cytomegalovirus (HCMV). An astrocytoma cell line (U373-MG) that is permissive for virus replication was permanently transfected with a construct bearing sequence from HCMV UL44 (coding for the major late DNA-binding protein, ppUL44, also known as pp52 or ICP36) in an antisense orientation and under the control of the immediate-early enhancer-promoter element. Upon HCMV infection at a high multiplicity, we found a marked reduction in UL44 protein products (the ICP36 family of proteins) in established cell transfectants and a strong inhibition of virus yield in infected-cell supernatants at two weeks postinfection, while herpes simplex virus replication was not affected. In infected cells, viral DNA replication was strongly inhibited. While gene products such as pUS22 and pUL32 were also inhibited, pUL123 and pUL82 accumulated in the infected cells over time. Our data suggest an essential role for the UL44 family of proteins in HCMV replication and represent a model of virus inhibition by virus-induced antisense RNA synthesis in genetically modified cells.

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confidence: 99%

Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene

Ripalti

Boccuni

Campanini

et al. 1995

J Virol

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“…Since Chang & Stoltzfus (1985) demonstrated the inhibitory effect of antisense RNA on Rous sarcoma virus gene expression, the effects have been investigated on several other viruses, including human immunodeficiency virus 1 (HIV-1 ; Chatterjee et al, 1992), adenovirus (Miroshnichenko et al, 1989), herpes simplex virus (Cantin et al, 1992), human cytomegalovirus (CMV ; Bryant & Sinclair, 1993), human T cell leukaemia virus type I (von Ruden & Gilboa, 1989), Moloney murine leukaemia virus (MoMLV ; Sullenger et al, 1990) and polyomavirus (Ottanvio et al, 1992). Successful inhibition of gene expression has been demonstrated for these viruses, especially for HIV-1.…”

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confidence: 99%

The inhibitory effects of antisense RNA on hepatitis B virus surface antigen synthesis.

Wu¹,

Gerber²

1997

Journal of General Virology

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Antisense RNA-mediated inhibition of gene expression has the potential for gene therapy of virus infections. We studied the inhibitory effect of antisense RNA directed against the hepatitis B virus (HBV) genome on the expression of the HBV surface antigen (HBsAg). Three prokaryotic antisense RNA expression constructs were produced which expressed antisense RNA complementary to the entire coding region (1n4 kb) and to 1n0 kb and 582 bp of the 5h region of HBsAg mRNA, respectively. In an in vitro translation system, all three antisense RNAs showed concentration-dependent inhibitory effects on translation of HBsAg mRNA. In a coupled in vitro transcription and translation system, concentration-dependent inhibition of HBsAg synthesis was observed for all above mentioned antisense RNAs. Three mammalian anti-

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Controlling Herpes Simplex Virus Infections: is Intracellular Immunization the Way of the Future?

Wong

Chatterjee

1992

Current Topics in Microbiology and Immunology

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Inhibition of adenovirus 5 replication in COS-1 cells by antisense RNAs against the viral E1a region

Cited by 14 publications

References 27 publications

Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene

Cytomegalovirus-mediated induction of antisense mRNA expression to UL44 inhibits virus replication in an astrocytoma cell line: identification of an essential gene

The inhibitory effects of antisense RNA on hepatitis B virus surface antigen synthesis.

Controlling Herpes Simplex Virus Infections: is Intracellular Immunization the Way of the Future?

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