Inhibition Effect of Vegetable Oils on the Mycelial Growth of Macrophomina phaseolina (Tassi.). Goid

Abstract

Macrophomina phaseolina (Tassi.). Goid is the etiological agent of one of the major diseases in cowpea bean crop, commonly known as gray rot stem. Due to the lack of registered phytosanitary products to control this disease, the search for alternative control methods is increasingly common. The objective of this study was to evaluate the effect of essential oils on mycelial growth of M. phaseolina. The oils tested were Mentha sp., Eucalyptus spp., Copaifera sp., and Lippia gracilis at concentrations of 0.4; 0.6; 0.8 and 1.0%. The work was conducted in Phytopathology Laboratory of the Federal University of Campina Grande. Daily measurements of the colony diameter were performed in two perpendicular directions until it filled the entire surface of the culture medium of one of the plates. The experiment was conducted in a completely randomized experimental design in a factorial arrangement 4 × 4 + 1 + 1, with sixteen treatments plus one negative control and one positive control, which consisted of the supplemental application in the medium (BDA) of the fungicide Sportak 450 EC (Procloraz), five replications, totaling 90 experimental plots. The data were interpreted through non-parametric analysis of variance and the means were compared by the Kruskal-wallis test, with a 5% probability of error and Scott Knott at 5% probability in cases where there were significant differences and data normality. The Mentha sp. essential oil and Lippia gracilis showed better results in inhibiting mycelial growth, while the Eucalyptus essential oil and Copaifera, although potentially promising, showed intermediate inhibition of fungal mycelial growth.

Management of Macrophomina phaseolina on Tomato using some Plant Extracts, Plant Oils, and some Biocontrol Agents

“…However, nigella and cactus oils were the least effect tested ones. These results are in harmony with Ugulino et al, 2018 who reported that using essential oils could inhibite the mycelial growth of Macrophomina phaseolina. Beg and Ahmed 2002, reported that Fusarium chlamydosporum and Macrophomina phaseolina were found to be highly sensitive to clove oil.…”

Macrophomina phaseolina: General Characteristics of Pathogenicity and Methods of Control

“…Moreover, some extracts were able to induce the activity of defense enzymes in soybean plants inoculated with M. phaseolina (Lorenzetti et al, 2018). Additionally, Lippia gracilis oil extract showed an important inhibitory effect on the mycelial growth of M. phaseolina, becoming a promising alternative as control method (Ugulino et al, 2018). Furthermore, exogenous application of the synthetic strigolactone (SL) GR24 suppressed M. phaseolina hyphal branching.…”

Control of Some Phytopathogenic Fungi Using Clove Essential Oil (Syzygium aromaticum L.)

“…For example, the total inhibition of F. verticillioides was achieved by França et al [39], Yamamoto-Ribeiro et al [40] and Bonfin et al [41] using the Palmarosa (Cymbopogon martinii), Ginger (Zingiber officinale) and Rosemary (Rosmarinus officinalis L.) essential oils at concentrations of 0.2%, 2.500 µg ml -1 (0.25%) and 150 µg ml -1 (0.015%), respectively. On control of M. phaseolina, Khaledi et al [42] and Ugulino et al [43] found maximum inhibition using Peppermint (Mentha piperita L.) and Alecrim-da-chapada (Lippia gracilis) at concentrations of 2.000 ppm (0.2%) and 0.4 to 1.0%, respectively.…”