Inhibition by Thyrocalcitonin (Calcitonin) of the Cyclic AMP-Mediated Stimulation of Thymocyte Proliferation by Epinephrine

Jf, Whitfield; Jp, MacManus; Dj, Gillan

doi:10.1055/s-0028-1094121

Cited by 12 publications

(5 citation statements)

References 2 publications

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“…In mixed cell preparations from rat thymus, salmon calcitonin inhibited apparently cAMP-mediated stimulation by epinephrine of thymocyte proliferation (19). On the other hand, the calcitonin did not affect [3H]thymidine incorporation into 8866 cells (not shown).…”

Section: Methodsmentioning

confidence: 91%

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Moran¹,

Hunziker²,

Fischer³

1978

Proc. Natl. Acad. Sci. U.S.A.

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Receptors for calcitonin, as assayed by the specific binding of 'SI-labeled salmon calcitonin and stimulation of cyclic AMP formation, were found in 886 cels derived from a human lymphoid line. The affinity of calcitonin from different species and of various analogues of human calcitonin for the binding sites and their ability to stimulate cyclic AMP formation were closely related to their hypocalcemic activity and presumably reflected biological properties of the hormones. Cell Culture, Isolation, and Incubation. The 8866 cells were derived from a patient with chronic myelogeneous leukemia (6) and were generously provided by S. J. Marx (NIAMDD). They were cultured in RPMI 1640 medium with 2 mM L-glutamine, supplemented with 10% fetal calf serum (vol/vol; Gibco, Glasgow, Scotland) and 0.5 ,ug of neomycin sulfate per ml, under a humid atmosphere of 95% air/5% CO2 at 370. Cells were fed two to three times weekly by 1:3 dilution in fresh growth medium. The cells were harvested at the late phase of exponential growth by sedimentation for 10 min at 300 X g at 40, washed three times in the incubation medium, and resuspended to a density of 107 cells per ml.The incubation medium contained 25 mM The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.

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Section: Methodsmentioning

confidence: 91%

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Moran¹,

Hunziker²,

Fischer³

1978

Proc. Natl. Acad. Sci. U.S.A.

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“…In 1973, Harrell, Binderman and Rodan confirmed these findings in isolated bone cells (Harrell, Binderman & Rodan, 1973). Other indirect evidence suggests that the cytoplasmic calcium activity is depressed by calcitonin (Nisket & Nordin, 1968;MacManus & Whitfield, 1970;Whitfield, MacManus & Gillan, 1971). Both reports concluded that calcitonin may act by inhibiting the active transport mechanism responsible for the cellular extrusion of calcium or by decreasing the concentration of intracellular calcium available for transport.…”

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confidence: 86%

Regulation of cellular calcium metabolism and calcium transport by calcitonin

Borle

1975

J. Membrain Biol.

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Calcitonin was studied in isolated kidney cells and in isolated mitochondria. A concentration of 10 ng/ml of synthetic calcitonin increases the cellular accumulation of 45Ca and the total cell calcium. The mitochondrial pool is increased several-fold. Kinetic analysis of the data shows that although the total cellular exchangeable calcium pool is enlarged, calcium influx and efflux are significantly depressed by calcitonin. The absence of phosphate or the presence of inhibitors of mitochondrial calcium transport completely abolish the effects of the hormone. In isolated mitochondria, the hormone stimulates the active calcium uptake and depresses the extramitochondrial calcium activity. Calcitonin counteracts the effects of cyclic AMP which stimulates the release of calcium from mitochondria and increases the extramitochondrial calcium activity. These data indicate that cellular calcium homeostasis is controlled by the mitochondrial calcium turnover. They suggest that calcitomin regulates the cell calcium metabolism and inhibits the transcellular calcium transport by stimulating the rate of calcium uptake by mitochondria which depresses cytoplasmic calcium activity.

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“…This localization of the initiating reactions is based on the fact that exogenous cyclic AMP maximally stimulates DNA synthesis and cell proliferation without entering the cell (6,7,9,10). Cyclic AMP (both endogenous and exogenous) may stimulate the activation site to produce intermediate compounds which enter the cell and start the calciuminsensitive DNA-synthetic reaction and the calcium-sensitive mitogenic process (Fig.…”

Section: Discussion the Additional Cyclic Ampmentioning

confidence: 99%

“…Since PGE1, like other cyclic AMP elevators, and cyclic AMP itself (6,(8)(9)(10)(11)13), rapidly (within 1 hr) stimulates the initiation of DNA synthesis (Table I ) , cyclic GMP and its mediator, calcium, may influence the prostaglandin mitogenic action by affecting this initial process. Despite the different elevations of the intracellular cyclic AMP level caused by 0.5 and 5.0 p g of PGEJml, DNA synthesis was equally stimulated, and was completely unaffected by calcium (Table I).…”

mentioning

confidence: 99%

“…The proliferative activity of the mitotically competent lymphoblasts, which constitute only 20% of a thymocyte population, was determined by adding the metaphase-1 Issued as NRCC No. 12474. blocking agent, colchicine (0.06 mM) to the medium and then measuring the subsequent accumulation, in the numerically constant cell population, of cells in colchicine metaphase (6)(7)(8)(9)(10)(11)(12)(13).…”

mentioning

confidence: 99%

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Calcium-Mediated Effects of Cyclic GMP on the Stimulation of Thymocyte Proliferation by Prostaglandin E1

Whitfield¹,

MacManus²

1972

Experimental Biology and Medicine

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Inhibition by Thyrocalcitonin (Calcitonin) of the Cyclic AMP-Mediated Stimulation of Thymocyte Proliferation by Epinephrine

Abstract: important mitogcnic action of parathyroid hormone, it is proposed that thyrocalcitonin (calcitonin) may bc a general regulator of cyclic AMP-controlled cell proliferation in vivo.

Cited by 12 publications

References 2 publications

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Calcitonin and calcium ionophores: cyclic AMP responses in cells of a human lymphoid line.

Regulation of cellular calcium metabolism and calcium transport by calcitonin

Calcium-Mediated Effects of Cyclic GMP on the Stimulation of Thymocyte Proliferation by Prostaglandin E1

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