Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: an<i>in vitro</i>study

Alım, Zuhal; Kılınç, Namık; Şengül, Bülent; Beydemır, Şükrü

doi:10.1080/14756366.2016.1250752

Cited by 51 publications

(33 citation statements)

References 54 publications

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“…The molar extinction coefficient is 6.22 M −1 cm −1 at pH 6.2 for NADPH. One enzyme unit was defined as the amount of enzyme that catalyzes the hydrolysis of 1 μmol of substrate at 25°C …”

Section: Methodsmentioning

confidence: 99%

“…According to this method, DE‐52 cellulose, Sephadex G‐100, and 2′5′‐ADP Sepharose 4B columns were used as anion exchange, gel filtration, and affinity chromatography, respectively. These techniques had been explained in the previous study . All experiments on purification were carried out at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…The elutions were performed by an increasing linear gradient of 0.25–2.5 mM NaCl (+4°C). The enzyme activity observed tubes were combined for other kinetic studie …”

Section: Methodsmentioning

confidence: 99%

“…One enzyme unit was defined as the amount of enzyme that catalyzes the hydrolysis of 1 mol of substrate at 25 • C. [29]…”

Section: Ar Activity Assaymentioning

confidence: 99%

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Phenolic compounds inhibit the aldose reductase enzyme from the sheep kidney

Demir

Işık

Gülçın

et al. 2017

J Biochem Mol Toxicol

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Aldose reductase (AR) is the key enzyme for the polyol pathway and responsible for sorbitol accumulation during the hyperglycemia. The present article focuses on the role of phenol, pyrogallol, hydroquinone, resorcinol, catechol, and phloroglucinol in in vitro inhibition of AR. For this purpose, AR was purified from the sheep kidney with 5.33 EU mg specific activity and 0.64% yield using several chromatographic methods. Various concentrations of the compounds were tested on in vitro AR activity. IC values were found for phenol, pyrogallol, hydroquinone, resorcinol, catechol, and phloroglucinol as 6.5, 1.13, 5.45, 2.21, 1.8, and 2.09 mM, respectively, and their K constant was calculated as 3.45 ± 0.92, 0.96 ± 0.28, 3.07 ± 0.46, 1.59 ± 0.43, 2.5 ± 0.35, and 2.54 ± 0.45 mM, respectively. Pyrogallol showed better inhibitory effect compared to the other compounds. The inhibition mechanisms of all compounds were noncompetitive. In the presents study, in vitro AR inhibition was examined by the phenolic compounds.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The elutions were performed by an increasing linear gradient of 0.25–2.5 mM NaCl (+4°C). The enzyme activity observed tubes were combined for other kinetic studie …”

Section: Methodsmentioning

confidence: 99%

“…One enzyme unit was defined as the amount of enzyme that catalyzes the hydrolysis of 1 mol of substrate at 25 • C. [29]…”

Section: Ar Activity Assaymentioning

confidence: 99%

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Phenolic compounds inhibit the aldose reductase enzyme from the sheep kidney

Demir

Işık

Gülçın

et al. 2017

J Biochem Mol Toxicol

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“…In the flowers of Musa spp., phenolic acids such as gallic acid, catechol, protocatechuic acid, gentisic acid, vanillic acid, caffeic acid, syringic acid, p‐coumaric acid, ferulic acid and epicatechin have been identified . These compounds have inhibitory activity on enzymes such as α‐amylase and aldose reductase, contributing to the reduction in glycaemic levels and the prevention of complications . Among these, vanillic acid has been shown to have a protective effect against hyperinsulinaemia, hyperglycaemia and hyperlipidaemia in rats fed high‐fat diets; the mechanism of action involves decreasing hepatic accumulation of nonesterified free fatty acid …”

Section: Introductionmentioning

confidence: 99%

Antidiabetic potential of Musa spp. inflorescence: a systematic review

Vilhena

Fachi

Marson

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives Extracts of parts Musa spp. have been used for the treatment of various diseases in traditional medicine. Studies have shown that these extracts have hypoglycaemic properties. The aim of this work was to gather evidence on the antidiabetic effects of Musa spp. inflorescence. Methods A systematic review was conducted with searches in three electronic databases, along with manual searches. Studies evaluating the antidiabetic properties of extracts of flower or bract of the genus Musa (in vitro or in vivo) were included. Key findings Overall, 16 studies were found. The reported assays were of hypoglycaemic effects, oral glucose tolerance, inhibitory activities in carbohydrate metabolism and digestive enzymes, enhanced glucose uptake activity and popular use of the extract in patients with diabetes type 2. In vitro studies showed that use of the extract was associated with antidiabetic effects (e.g. increased glucose uptake and inhibition of carbohydrate digestion enzymes). In induced diabetic models, Musa spp. extracts showed dose‐dependent glycaemic level reductions compared with pharmacological drugs (P < 0.05). Summary In general, promising results regarding antidiabetic activity were found for inflorescence of Musa spp., suggesting that this plant could represent a natural alternative therapy for treating diabetes mellitus type 2.

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Antidiabetic potential: In vitro inhibition effects of bromophenol and diarylmethanones derivatives on metabolic enzymes

Demir

Taslimi

Özaslan

et al. 2018

Archiv der Pharmazie

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Aldose reductase converts glucose to sorbitol in the polyol pathway. It is an important enzyme to prevent diabetic complications. In this study, we studied the inhibitory effects of bromophenol derivatives on aldose reductase (AR), α-glucosidase, and α-amylase enzymes. In the bromophenols series, compound 1f showed the maximum inhibition effect against AR with a K i value of 0.05 ± 0.01 μM, while compound 1d showed the lowest inhibition effect against AR with a K i value of 1.13 ± 0.99 μM. In addition, α-amylase from porcine pancreas and α-glucosidase from Saccharomyces cerevisiae were used as enzymes. In this study, all compounds were tested for the inhibition of the α-glucosidase enzyme and demonstrated efficient inhibition profiles with K i values in the range of 43.62 ± 5.28 to 144.37 ± 16.37 nM against α-glucosidase.Additionally, these compounds were tested against the α-amylase enzyme, which determined an effective inhibition profile with IC 50 values in the range of 9.63-91.47 nM. These compounds can be selective inhibitors of AR, α-glucosidase, and α-amylase enzymes as antidiabetic agents. K E Y W O R D S α-amylase, α-glucosidase, aldose reductase, bromophenol, enzyme inhibition Arch Pharm Chem Life Sci. 2018;351:e1800263.wileyonlinelibrary.com/journal/ardp AR activity was assayed according to previous studies and it measured the decrease of NADPH at 340 nm spectrophotometrically. [45,46] | Enzyme purificationEnzyme purification procedures were done according to previous studies. [47,48] | Protein determinationThe protein concentration was measured according to the Bradford method by using with Coomassie Brilliant Blue G-250. [49] 4.2.7 | SDS polyacrylamide gel electrophoresisAfter the enzyme purification, the purity degree of the enzyme was controlled according to Laemmli's method [50] by using 3-8% batch sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis process was carried out according to our previous papers. [51][52][53] The gel was stained with the silver staining method and photographed after staining. CONFLICT OF INTERESTThe authors declare that there are no conflicts of interest. ORCIDParham Taslimi

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Inhibition behaviours of some phenolic acids on rat kidney aldose reductase enzyme: anin vitrostudy

Cited by 51 publications

References 54 publications

Phenolic compounds inhibit the aldose reductase enzyme from the sheep kidney

Phenolic compounds inhibit the aldose reductase enzyme from the sheep kidney

Antidiabetic potential of Musa spp. inflorescence: a systematic review

Antidiabetic potential: In vitro inhibition effects of bromophenol and diarylmethanones derivatives on metabolic enzymes

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