Inhibiting Pathogen Surface Adherence by Multilayer Polyelectrolyte Films Functionalized with Glucofuranose Derivatives

Abstract

Inhibiting pathogenic bacterial adherence on surfaces is an ongoing challenge to prevent the development of biofilms. Multilayer polyelectrolyte films are feasible antibacterial materials. Here, we have designed new films made of carbohydrate polyelectrolytes to obtain antibacterial coatings that prevent biofilm formation. The polyelectrolyte films were constructed from poly(maleic anhydride- alt-styrene) functionalized with glucofuranose derivatives and quaternized poly(4-vinylpyridine) N-alkyl. These films prevent Pseudomonas aeruginosa and Salmonella Typhimurium, two important bacterial contaminants in clinical environments, from adhering to surfaces. When the film was composed of more than 10 layers, the bacterial population was greatly reduced, while the bacteria remaining on the film were morphologically damaged, as atomic force microscopy revealed. The antibacterial capacity of the polyelectrolyte films was determined by the combination of thickness, wettability, surface energy, and most importantly, the conformation that polyelectrolytes adopt the function of nature of the carbohydrate group. This polyelectrolyte film constitutes the first green approach to preventing pathogenic bacterial surface adherence and proliferation without killing the bacterial pathogen.

Multicomponent Approach to Homo- and Hetero-Multivalent Glycomimetics Bearing Rare Monosaccharides

“…Scale up: from 2.5 mmol (650 mg) 6-OH-isop-Gal, 63 % (408 mg) of 11 was obtained over three steps; from 3.5 mmol (910 mg) 6-OH-isop-Gal, 59 % (535 mg) of 11 was obtained over three steps; from 6.4 mmol (1664 mg) 6-OH-isop-Gal, 45 % (746 mg) of 11 was obtained over three steps. 43 1,2;5,6-di-O-isopropylidene-α-D-Glucofuranose (520 mg, 2.00 mmol) was dissolved in dry DCM (22 mL) under nitrogen at -20 °C and then pyridine (432 μL) anhydride of trifluoromethanesulfonic acid (497 μL) dissolved in dry DCM (5.5 mL) were added slowly over 20 min. After 1 h of stirring the reaction mixture was rinsed with 24 mL water, 24 mL KHSO4 (10 %), 16 mL sat.…”

Multilayered Curcumin-loaded Hydrogel Microcarriers With Antimicrobial Function

“…In recent years, bacterial infections have become a major problem worldwide [1,2]. Microbes are becoming more and more resistant to antibiotics and other antimicrobial agents.…”

Liquid-like Polymer Coating as a Promising Candidate for Reducing Electrode Contamination and Noise in Complex Biofluids

“…6,14−17 Despite tremendous advances in the electrode sensing technology, these sensors still suffer from nonspecific adhesion of proteins, bacteria, and other biological components that cause biofouling in many applications. 18,19 This biofouling hinders the effective coupling of the target biomarkers onto the electrode surface and hence seriously interferes with the long-term stability of sensing. 20−22 There-fore, it is imperative to develop a functional electrode sensing system that eliminates nonspecific adhesion and contamination to maintain electrochemical performance in complex biofluids.…”

“…For decades, bioelectrodes have been recognized as one of the most promising artificial biological devices for biomedical or environmental applications. − At present, bioelectrodes are widely used in biosensing, drug delivery, capture of circulating tumor cells, and euroregulation , due to their many potential advantages such as high efficiency and speed. In particular, representative electrochemical sensing devices, integrated three-electrode systems (ITESs), with the characteristics of simplicity and portability, offer promising potential for rapid and sensitive monitoring of biomarkers such as hydrogen peroxide, glucose, and protein. − Furthermore, ITESs are widely used for in situ marker monitoring and analysis in various fields with clinical, food, environmental, and biological applications. ,− Despite tremendous advances in the electrode sensing technology, these sensors still suffer from nonspecific adhesion of proteins, bacteria, and other biological components that cause biofouling in many applications. , This biofouling hinders the effective coupling of the target biomarkers onto the electrode surface and hence seriously interferes with the long-term stability of sensing. − Therefore, it is imperative to develop a functional electrode sensing system that eliminates nonspecific adhesion and contamination to maintain electrochemical performance in complex biofluids.…”