Inhibiting effects of <i>Streptococcus salivarius</i> on competence‐stimulating peptide‐dependent biofilm formation by <i>Streptococcus mutans</i>

Tamura, Shohei; Yonezawa, Hideo; Motegi, Mizuho; Nakao, Ryoma; Yoneda, Saori; Watanabe, Haruo; Yamazaki, T.; Senpuku, Hidenobu

doi:10.1111/j.1399-302x.2008.00489.x

Cited by 70 publications

(52 citation statements)

References 52 publications

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“…Mixed cultures of S. mutans with other oral bacteria, whether commensal species such as Streptococcus salivarius or pathogens such as Porphyromonas gingivalis and Treponema denticola, present situations where the competing species inhibit S. mutans' ability to regulate QS-controlled behaviors and do so via an extracellular factor (304,305). Though mechanisms for this inhibition have not been discerned, the inhibitory activity can be abolished by boiling active supernatants (305) or by supplementing cultures with excessive S. mutans CSP (304). Clearly, niches where large amounts of microbial interaction and competition exist may provide excellent examples of natural QS antagonism.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

678

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

678

556

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“…Although many studies have described the mechanisms of streptococcal adhesion (17,24,37,41) and coaggregation among oral bacteria (25,38), the subsequent process of bacterial accumulation, proliferation, and biofilm formation leading to functional heterogeneous species in the organized sessile community is poorly understood, especially in the presence of sucrose that induces cariogenicity. However, it is likely that the cooperative interactions between mutans streptococci and other oral streptococci play important roles in the development of dental biofilm and caries in the oral cavity (27,48,50,53).…”

mentioning

confidence: 99%

Inhibition of Streptococcus mutans Biofilm Formation by Streptococcus salivarius FruA

Ogawa

Furukawa

Fujita

et al. 2011

Appl Environ Microbiol

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The oral microbial flora consists of many beneficial species of bacteria that are associated with a healthy condition and control the progression of oral disease. Cooperative interactions between oral streptococci and the pathogens play important roles in the development of dental biofilms in the oral cavity. To determine the roles of oral streptococci in multispecies biofilm development and the effects of the streptococci in biofilm formation, the active substances inhibiting Streptococcus mutans biofilm formation were purified from Streptococcus salivarius ATCC 9759 and HT9R culture supernatants using ion exchange and gel filtration chromatography. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry analysis was performed, and the results were compared to databases. The S. salivarius HT9R genome sequence was determined and used to indentify candidate proteins for inhibition. The candidates inhibiting biofilms were identified as S. salivarius fructosyltransferase (FTF) and exo-beta-D-fructosidase (FruA). The activity of the inhibitors was elevated in the presence of sucrose, and the inhibitory effects were dependent on the sucrose concentration in the biofilm formation assay medium. Purified and commercial FruA from Aspergillus niger (31.6% identity and 59.6% similarity to the amino acid sequence of FruA from S. salivarius HT9R) completely inhibited S. mutans GS-5 biofilm formation on saliva-coated polystyrene and hydroxyapatite surfaces. Inhibition was induced by decreasing polysaccharide production, which is dependent on sucrose digestion rather than fructan digestion. The data indicate that S. salivarius produces large quantities of FruA and that FruA alone may play an important role in multispecies microbial interactions for sucrose-dependent biofilm formation in the oral cavity.

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“…In healthy human beings, S. salivarius is the main component in biofilm that forms on the buccal mucosa, tongue, dorsum epithelial, and pharynx mucosa. Some strains of S. salivarius on the tongue release bacteriocins that are toxic to oral flora bacteria and that change characteristics from commensal to pathogenic [4,5]. The literature shows S. salivarius as toxic to the oral streptococci involved in tooth decay, including Streptococcus mutans, Streptococcus sobrinus, and Streptococcus pyogenes, and to the pathogens involved in periodontitis [6].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Potential of Streptococcus Salivarius Isolated From the Saliva and Tongue Dorsum to Inhibit the Growth of Fusobacterium Nucleatum

2017

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Objective: Analyzing the potential of S. salivarius isolated from the saliva and tongue dorsum of adults to inhibit the growth of Fusobacterium nucleatum.Methods: Polymerase chain reaction, deferred antagonism test, and well-diffused agar test.Results: Inhibition of the growth F. nucleatum by S. salivarius isolated from the tongue dorsum (p>0.05). No inhibition to the growth of F. nucleatum by S. salivarius isolated from the saliva. No inhibition to the growth of F. nucleatum by the protein produced by S. salivarius. Conclusions:The growth of F. nucleatum was not inhibited by S. salivarius isolated from the saliva but by S. salivarius isolated from the dorsum of the tongue.

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Inhibiting effects of Streptococcus salivarius on competence‐stimulating peptide‐dependent biofilm formation by Streptococcus mutans

Cited by 70 publications

References 52 publications

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Inhibition of Streptococcus mutans Biofilm Formation by Streptococcus salivarius FruA

Analysis of the Potential of Streptococcus Salivarius Isolated From the Saliva and Tongue Dorsum to Inhibit the Growth of Fusobacterium Nucleatum

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