Objective: Tooth decay or the caries process is a common dental problem that affects millions of people worldwide. Many risk factors are modifiable,while others are not (e.g., genetic factors). Polymorphism of the enamelin (ENAM) gene, which is required to ensure production of an essentialprotein for enamel development, may pose as a risk factor for the caries process. This study sought to investigate the possibility of ENAM C2452Tpolymorphism acting as a risk factor in the caries process.Methods: The polymerase chain reaction–restriction fragment length polymorphism method was employed to evaluate DNA samples taken from 95subjects with a high caries prevalence and 89 control subjects for ENAM C2452T polymorphism.Results: Based on Chi-squared tests, there were significant genotype and allele distribution differences between the group with a high cariesprevalence and the control group (p=0.005 and p=0.007). Polymorphism in this context may, therefore, serve as a risk factor for caries onset andprogression (OR: 3.62).Conclusion: ENAM C2452T polymorphism is related to the caries process and may constitute a risk factor.
Objective: To analyze the inhibitory potential of S. salivarius isolated from saliva and the dorsum of the tongue, and the protein it produces, in inhibiting the growth of Aggregatibacter actinomycetemcomitans.Methods: Examine the inhibition zone of A. actinomycetemcomitans formed by the treatment of S. salivarius isolated from saliva and the tongue dorsum, along with the protein produced, in 10 participants using a deferred antagonism test with well-diffused gelatin.
Results:The inhibitory zone of S. salivarius isolated from saliva and the tongue against A. actinomycetemcomitans is insignificantly different (p≥0.05). There is no inhibition of the growth of A. actinomycetemcomitans shown by whole-cell and spent medium proteins of S. salivarius.
Conclusion:S. salivarius isolated from both saliva and the tongue dorsum is able to inhibit the growth of A. actinomycetemcomitans, but not the protein they produce.
Result:The maximum inhibitor diameter for each isolation was 11.17 mm at 10 10 CFU for the dorsum of tongue isolates and 8.17 mm at 10 9 CFU for saliva isolates.Conclusions: Clinical S. salivarius isolates from the dorsum of tongue had greater potential for inhibiting E. faecalis growth compared to the saliva isolates and control bacteria.
Objective: The aim of this study is to analyze the inhibitory potential of Streptococcus salivarius, after it has been isolated from saliva and the tongue's dorsum, on the growth of Streptococcus mutans.Method: Polymerase chain reaction, SDS PAGE, Bradford test, deferred antagonism test, and well-diffusion test were used.Result: S. salivarius isolated from saliva and the tongue's dorsum inhibited the growth of S. mutans, but it was not the case with S. salivarius ATCC 13419 (p<0.05). However, there was no significant difference between the inhibition of S. mutans from S. salivarius isolated from saliva and the tongue's dorsum (p>0.05).
Conclusion:S. salivarius isolated from saliva and the tongue's dorsum can inhibit the growth of S. mutans, but there was no significant difference in the inhibitory potential of S. salivarius isolated from saliva on the tongue's dorsum.
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