Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage

Chhibber, Sanjay; Nag, Deepika; Bansal, Shruti

doi:10.1186/1471-2180-13-174

Cited by 80 publications

(48 citation statements)

References 22 publications

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“…Then, 200 μL of the suspension was added to microtiter plate wells. After incubation at 37 ° C for 24 h, planktonic cells were removed and the biofilms were treated with phage, antibiotic, and their combination [Chhibber et al, 2013]. The phage and ampicillin were used at different MOIs (1 and 100) and concentrations (including an MIC of 16 µg/mL and about 15-fold higher than the MIC value), respectively [Rahman et al, 2011].…”

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…The isolated phage's ability to remove P. mirabilis biofilm was assessed alone or combined with the antibiotic according to Chhibber et al [2013], with some modifications in microtiter plates. A bacterial suspension (1.5 × 10 8 CFU/mL) was prepared in the medium (BHI broth supplemented with 1% saccharose).…”

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

“…Treatment of biofilms with phages might be difficult due to emergence of phage-resistant strains and the poor penetration of phages within older biofilms caused by the presence of a large number of metabolically inactive cells. Thus, biofilm treatment with a combination of chemically distinct antimicrobials might be an effective strategy for eliminating these problems [Chhibber et al, 2013]. Similar to multidrug therapy, combined phage-drug therapy is less likely to fail due to resistance, as bacteria resistant to one agent can still be killed by the second [Carson et al, 2010;Kirby, 2012;Nzakizwanayo et al, 2016].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of Proteus mirabilis Isolated from Urinary Tract Infection

2018

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Proteus mirabilis is one of the most common causes of urinary tract infection (UTI), particularly in patients undergoing long-term catheterization. Phage vB_PmiS-TH was isolated from wastewater with high lytic activity against P. mirabilis (TH) isolated from UTI. The phage had rapid adsorption, a large burst size (∼260 PFU per infected cell), and high stability at a wide range of temperatures and pH values. As analyzed by transmission electron microscopy, phage vB_PmiS-TH had an icosahedral head of ∼87 × 62 nm with a noncontractile tail about 137 nm in length and 11 nm in width. It belongs to the family Siphoviridae. Combination of the phage vB_PmiS-TH with ampicillin had a higher removal activity against planktonic cells of P. mirabilis (TH) than the phage or the antibiotic alone. Combination of the phage at a multiplicity of infection of 100 with a high dose of ampicillin (246 µg/mL) showed the highest biofilm removal activity after 24 h. This study demonstrates that using a combination of phage and antibiotic could be significantly more effective against planktonic and biofilm forms of P. mirabilis (TH).

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Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of Proteus mirabilis Isolated from Urinary Tract Infection

2018

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“…Hence, novel antimicrobial agents which selectively reduce the virulence of pathogens rather than inducing antimicrobial resistance are the need of the hour [16]. Antibiofilm agents are one such a kind which selectively eradicate the persistent biofilms thereby facilitate the diffusion of antibiotics into the complex biofilm ambience [17,18]. Identification and characterization of new biosurfactants and antibiofilm agents with profuse biological activities will aid mankind to combat recurrent infections caused by drug resistant biofilm forming pathogens [19].…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Padmavathi

Pandian

2014

Indian J Microbiol

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Coral Associated Bacteria (CAB) (N = 22) isolated from the mucus of the coral Acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. Six CAB (U7, U9, U10, U13, U14, and U16) were found to produce biosurfactants and were identified by 16S ribosomal RNA gene sequencing as Providencia rettgeri, Psychrobacter sp., Bacillus flexus, Bacillus anthracis, Psychrobacter sp., and Bacillus pumilus respectively. Their cell surface hydrophobicity was determined by Microbial adhesion to hydrocarbon assay and the biosurfactants produced were extracted and characterized by Fourier Transform Infrared spectroscopy. Since the biosurfactants are known for their surface modifying capabilities, antibiofilm activity of positive isolates was evaluated against biofilm forming Pseudomonas aeruginosa ATCC10145. Stability of the active principle exhibiting antibiofilm activity was tested through various temperature treatments ranging from 60 to 100°C and Proteinase K treatment. CAB isolates U7 and U9 exhibited stable antibiofilm activity even after exposure to higher temperatures which is promising for the development of novel antifouling agents for diverse industrial applications. Further, this is the first report on biosurfactant production by a coral symbiont.

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“…[9][10][11] With this background, bacteriophages have a great unexplored potential, in both preclinical and clinical research.…”

Section: Introductionmentioning

confidence: 99%

Experimental approach for bacteriophage susceptibility testing of planktonic and sessile bacterial populations – Study protocol

Săndulescu

Popa

et al. 2014

GERMS

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Introduction Antimicrobial resistance is a growing threat for all clinical branches. This phenomenon poses important challenges in controlling infectious diseases. However, multidrug resistance is not the only issue, as bacteria that are otherwise susceptible to common antibiotics express other patterns for evading antibiotherapy, for example they can aggregate within a self-produced matrix to form biofilm.Methods We intend to perform a prospective laboratory study of the germs isolated from different samples collected from patients admitted with infectious pathology in reference hospitals in Romania. We will perform antibiotic resistance testing as well as phage testing, both on solid and liquid growth medium, for Staphylococcus spp., Enterococcus spp., and Pseudomonas spp. We intend to collect data for 150 patients with different infections with these identified pathogens.Phage susceptibility testing will be performed using 5 types of strain-specific bacteriophage mixtures: PYO, INTESTI, STAPHYLOCOCCAL (Eliava BioPreparations, Tbilisi, Georgia), PHAGYO, PHAGESTI (JSC "Biochimpharm", Tbilisi, Georgia). For phage-susceptible strains, we will evaluate biofilm formation in the presence of phages, as well as phage effect on already formed biofilm.Expected results Through this study, we intend to provide the first set of results on bacteriophagesusceptibility of bacteria isolated from patients with hard to treat infections, from reference hospitals in Romania. By evaluating a large number of bacterial strains we aim to predict and project biofilm kinetics, while adding binary phage dilutions at key timepoints during biofilm formation.

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Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage

Cited by 80 publications

References 22 publications

Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of Proteus mirabilis Isolated from Urinary Tract Infection

Isolation and Characterization of a Lytic Bacteriophage (vB_PmiS-TH) and Its Application in Combination with Ampicillin against Planktonic and Biofilm Forms of Proteus mirabilis Isolated from Urinary Tract Infection

Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar

Experimental approach for bacteriophage susceptibility testing of planktonic and sessile bacterial populations – Study protocol

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