Inhibiting bacterial biofilm formation by stimulating c-di-GMP regulation using citrus peel extract from Jeju Island

Kim, Hanshin; Ham, So-Young; Ryoo, Hwa-Soo; Kim, Do-Hyung; Yun, Eun-Tae; Park, Hee-Deung; Park, Jeong Hoon

doi:10.1016/j.scitotenv.2023.162180

Cited by 10 publications

(2 citation statements)

References 55 publications

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“…of transcriptional regulators, serves as the central regulator of T3SS gene expression (Janssen et al, 2020). In the latest study, citrus peel extract from Jeju Island (CPEJ) can inhibit bacterial biofilm formation (Kim et al, 2023). The result shows that CPEJ can significantly reduce the c-di-GMP level through increased phosphodiesterase activity, which suggests the potential of CPEJ for applications in clinical.…”

Section: Biofilm Infections Microscopic Changes and Paraclinical Chan...mentioning

confidence: 99%

A growing battlefield in the war against biofilm-induced antimicrobial resistance: insights from reviews on antibiotic resistance

Pai,

Patil,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

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Biofilms are a common survival strategy employed by bacteria in healthcare settings, which enhances their resistance to antimicrobial and biocidal agents making infections difficult to treat. Mechanisms of biofilm-induced antimicrobial resistance involve reduced penetration of antimicrobial agents, increased expression of efflux pumps, altered microbial physiology, and genetic changes in the bacterial population. Factors contributing to the formation of biofilms include nutrient availability, temperature, pH, surface properties, and microbial interactions. Biofilm-associated infections can have serious consequences for patient outcomes, and standard antimicrobial therapies are often ineffective against biofilm-associated bacteria, making diagnosis and treatment challenging. Novel strategies, including antibiotics combination therapies (such as daptomycin and vancomycin, colistin and azithromycin), biofilm-targeted agents (such as small molecules (LP3134, LP3145, LP4010, LP1062) target c-di-GMP), and immunomodulatory therapies (such as the anti-PcrV IgY antibodies which target Type IIIsecretion system), are being developed to combat biofilm-induced antimicrobial resistance. A multifaceted approach to diagnosis, treatment, and prevention is necessary to address this emerging problem in healthcare settings.

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Section: Biofilm Infections Microscopic Changes and Paraclinical Chan...mentioning

confidence: 99%

A growing battlefield in the war against biofilm-induced antimicrobial resistance: insights from reviews on antibiotic resistance

Pai,

Patil,

Liu

et al. 2023

Front. Cell. Infect. Microbiol.

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show abstract

“…Recent evidence obtained from one of the dominant marine Vibrio spp., Vibrio parahaemolyticus , has also made it clear that high c-di-GMP favors biofilm formation ( Kimbrough et al, 2020 ; Martinez-Mendez et al, 2021 ). In addition, interfering with c-di-GMP metabolism and signaling has been shown to be a promising way to control biofilm development ( Liu et al, 2022 ; Kim et al, 2023 ). Therefore, modulation of the intracellular c-di-GMP pool is a key regulator of bacterial adaptation to sessile and motile lifestyles in response to complex environmental and internal cues.…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins in Vibrio alginolyticus unveils key diguanylate cyclases controlling multiple biofilm-associated phenotypes

Gong,

Zeng,

Chen

et al. 2023

Front. Microbiol.

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The biofilm lifestyle is critical for bacterial survival and proliferation in the fluctuating marine environment. Cyclic diguanylate (c-di-GMP) is a key second messenger during bacterial adaptation to various environmental signals, which has been identified as a master regulator of biofilm formation. However, little is known about whether and how c-di-GMP signaling regulates biofilm formation in Vibrio alginolyticus, a globally dominant marine pathogen. Here, a large set of 63 proteins were predicted to participate in c-di-GMP metabolism (biosynthesis or degradation) in a pathogenic V. alginolyticus strain HN08155. Guided by protein homology, conserved domains and gene context information, a representative subset of 22 c-di-GMP metabolic proteins were selected to determine which ones affect biofilm-associated phenotypes. By comparing phenotypic differences between the wild-type and mutants or overexpression strains, we found that 22 c-di-GMP metabolic proteins can separately regulate different phenotypic outputs in V. alginolyticus. The results indicated that overexpression of four c-di-GMP metabolic proteins, including VA0356, VA1591 (CdgM), VA4033 (DgcB) and VA0088, strongly enhanced rugose colony morphotypes and strengthened Congo Red (CR) binding capacity, both of which are indicators of biofilm matrix overproduction. Furthermore, rugose enhanced colonies were accompanied by increased transcript levels of extracellular polysaccharide (EPS) biosynthesis genes and decreased expression of flagellar synthesis genes compared to smooth colonies (WTpBAD control), as demonstrated by overexpression strains WTp4033 and ∆VA4033p4033. Overall, the high abundance of c-di-GMP metabolic proteins in V. alginolyticus suggests that c-di-GMP signaling and regulatory system could play a key role in its response and adaptation to the ever-changing marine environment. This work provides a robust foundation for the study of the molecular mechanisms of c-di-GMP in the biofilm formation of V. alginolyticus.

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Use of Lemon Peel Extract as Antimicrobial Supported on Eco-friendly Polyvinyl Alcohol/Polydimethylsiloxane Sponges

García Fernández,

Levy,

Salustro

et al. 2024

J Polym Environ

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Inhibiting bacterial biofilm formation by stimulating c-di-GMP regulation using citrus peel extract from Jeju Island

Cited by 10 publications

References 55 publications

A growing battlefield in the war against biofilm-induced antimicrobial resistance: insights from reviews on antibiotic resistance

A growing battlefield in the war against biofilm-induced antimicrobial resistance: insights from reviews on antibiotic resistance

Bioinformatic and functional characterization of cyclic-di-GMP metabolic proteins in Vibrio alginolyticus unveils key diguanylate cyclases controlling multiple biofilm-associated phenotypes

Use of Lemon Peel Extract as Antimicrobial Supported on Eco-friendly Polyvinyl Alcohol/Polydimethylsiloxane Sponges

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