Inhibiting Amadori-modified albumin formation improves biomarkers of podocyte damage in diabetic rats

Cohen, Morrel H.; Shearman, Clyde W.

doi:10.1002/phy2.83

Cited by 3 publications

(2 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in serum GA concentration is followed by nephrin level increase in diabetic murine. Conversely, if serum GA level is reduced by controlling hyperglycemia, the urinary nephrin also decreased [32].…”

Section: Correlation Between Serum Glycated Albumin With Urinary Nephmentioning

confidence: 99%

See 1 more Smart Citation

Correlation between Uncontrolled Blood Glucose and Oxidative Stress with Urinary Nephrin Level in Type 2 Diabetes Mellitus

Aprilia¹,

Angraeni²,

Decroli³

et al. 2019

Proceedings of the Proceedings of the 1st EAI International Conference on Medical and Health Research, ICoMHER November 13-14th

View full text Add to dashboard Cite

Diabetes mellitus (DM) is one of the world's health problem with increasing prevalence and important medical and social impacts. Diabetes mellitus causes micro and macrovascular complications depending on the presence of blood glucose level disorder. Impaired glucose levels have two components, chronic hyperglycemia and acute fluctuations of blood glucose levels from peak to nadir. These two components cause DM with two main mechanisms, protein glycation, and oxidative stress. Glycated albumin describes uncontrolled medium range glucose level while malondialdehyde reflects oxidative stress. Nephrin is one of the damage markers of podocyte that represents diabetic kidney disease. To find out the correlation between uncontrolled blood glucose with urinary nephrin level in a patient with type 2 diabetes mellitus. This is an observational study with the cross-sectional method. Subjects are 30 types 2 DM patients. Patients serum glycated albumin (GA) are measured, along with serum malondialdehyde (MDA) and urinary nephrin. Among 30 types 2 DM patients, there is a significant increase in GA and MDA level. Mean serum GA level is 20,87% (5,91) %, serum MDA level is 9,24 (5,59-13,59) and urinary nephrin is 369,25 (6-3952) ng/ml. There is a moderate correlation between serum GA with urinary nephrin (p=0,027) and moderate correlation between serum MDA and urinary nephrin (p=0,003). There is a correlation between uncontrolled blood glucose with urinary nephrin level in type 2 diabetes mellitus.

show abstract

Section: Correlation Between Serum Glycated Albumin With Urinary Nephmentioning

confidence: 99%

“…A study by Cohen et al (2013) measured the level of glycated albumin, creatinine, albumin, and nephrin in diabetic murine compared to nondiabetic marines. This study demonstrated a positive linear correlation between GA and urinary nephrin (p<0,05 and r=0,58).…”

Section: Correlation Between Serum Glycated Albumin With Urinary Nephmentioning

confidence: 99%

Correlation between Uncontrolled Blood Glucose and Oxidative Stress with Urinary Nephrin Level in Type 2 Diabetes Mellitus

Aprilia¹,

Angraeni²,

Decroli³

et al. 2019

Proceedings of the Proceedings of the 1st EAI International Conference on Medical and Health Research, ICoMHER November 13-14th

View full text Add to dashboard Cite

show abstract

Hypoxia inducible factor-1α mediates the profibrotic effect of albumin in renal tubular cells

Wang

Zhang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Proteinuria is closely associated with the progression of chronic kidney diseases (CKD) by producing renal tubulointerstitial fibrosis. Over-activation of hypoxia inducible factor (HIF)-1α has been implicated in the progression of CKD. The present study tested the hypothesis that HIF-1α mediates albumin-induced profibrotic effect in cultured renal proximal tubular cells. Incubation of the cells with albumin (40 μg/ml) for 72 hrs significantly increased the protein levels of HIF-1α, tissue inhibitor of metalloproteinase (TIMP)-1 and collagen-I, which were blocked by HIF-1α shRNA. Albumin also stimulated an epithelial-mesenchymal transition (EMT) as indicated by the decrease in epithelial marker E-cadherin, and the increase in mesenchymal markers α-smooth muscle actin and fibroblast-specific protein 1. HIF-1α shRNA blocked albumin-induced changes in these EMT markers as well. Furthermore, albumin reduced the level of hydroxylated HIF-1α, indicating an inhibition of the activity of prolyl-hydroxylases, enzymes promoting the degradation of HIF-1α. An anti-oxidant ascorbate reversed albumin-induced inhibition of prolyl-hydroxylase activity. Overexpression of prolyl-hydroxylase 2 (PHD2) transgene, a predominant isoform of PHDs in renal tubules, to reduce HIF-1α level significantly attenuated albumin-induced increases in TIMP-1 and collagen-I levels. These results suggest that albumin-induced oxidative stress inhibits PHD activity to accumulate HIF-1α, which mediates albumin-induced profibrotic effects in renal tubular cells.

show abstract

Growth arrest specific 2–like protein 1 expression is upregulated in podocytes through advanced glycation end-products

Liebisch

Bondeva

Franke

et al. 2016

Nephrol. Dial. Transplant.

View full text Add to dashboard Cite

show abstract

Inhibiting Amadori-modified albumin formation improves biomarkers of podocyte damage in diabetic rats

Cited by 3 publications

References 61 publications

Correlation between Uncontrolled Blood Glucose and Oxidative Stress with Urinary Nephrin Level in Type 2 Diabetes Mellitus

Correlation between Uncontrolled Blood Glucose and Oxidative Stress with Urinary Nephrin Level in Type 2 Diabetes Mellitus

Hypoxia inducible factor-1α mediates the profibrotic effect of albumin in renal tubular cells

Growth arrest specific 2–like protein 1 expression is upregulated in podocytes through advanced glycation end-products

Contact Info

Product

Resources

About