Inheritance of steroidal glycoalkaloids in potato tuber flesh

Peng, Zhen; Wang, Pei; Tang, Dié; Shang, Yi; Li, Canhui; Huang, Sanwen; Zhang, Chunzhi

doi:10.1016/s2095-3119(19)62718-8

Cited by 10 publications

(15 citation statements)

References 28 publications

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“…2a). We further amplified the collinear region, including the 2100 bp promoter and the 1810 bp gene coding sequence, where NSF/Sli is located in E172 29 (a self-compatible F 1 individual generated by crossing a self-incompatible diploid line E and chc 525-3). The promoter sequences in E172 and M6 are identical to that of RH, but there are five DNA base differences within the NSF/Sli coding region, resulting in two amino acid changes (C214R and Q249R) in M6 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Zhang

et al. 2021

Nat Commun

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Potato is the third most important staple food crop. To address challenges associated with global food security, a hybrid potato breeding system, aimed at converting potato from a tuber-propagated tetraploid crop into a seed-propagated diploid crop through crossing inbred lines, is under development. However, given that most diploid potatoes are self-incompatible, this represents a major obstacle which needs to be addressed in order to develop inbred lines. Here, we report on a self-compatible diploid potato, RH89-039-16 (RH), which can efficiently induce a mating transition from self-incompatibility to self-compatibility, when crossed to self-incompatible lines. We identify the S-locusinhibitor (Sli) gene in RH, capable of interacting with multiple allelic variants of the pistil-specific S-ribonucleases (S-RNases). Further, Sli gene functions like a general S-RNase inhibitor, to impart SC to RH and other self-incompatible potatoes. Discovery of Sli now offers a path forward for the diploid hybrid breeding program.

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Section: Resultsmentioning

confidence: 99%

A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Zhang

et al. 2021

Nat Commun

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“…We found different expression patterns of various UDP-glycosyltransferases (see Additional File 5 ), especially UDP-glycosyltransferase 90A1 (log2 FC = -3.01), which confirmed different GA landscapes between the samples and may affect PP of PLE. It was recently demonstrated that the pattern of GAs in progeny derived from crosses with S. chacoense segregates and may be regulated by 38 genes that are located on chromosome VIII and coexpressed with GAME genes [ 31 ]. Comprehensive analysis of DEGs, the GO enrichment, pointing significant participation of metabolic processes (BP-30.9%) and catalytic activity (MF-62.2%) as most differentiating the bulks.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and proteomic insights into phytotoxic activity of interspecific potato hybrids with low glycoalkaloid contents

et al. 2021

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Background Glycoalkaloids are bioactive compounds that contribute to the defence response of plants against herbivore attack and during pathogenesis. Solanaceous plants, including cultivated and wild potato species, are sources of steroidal glycoalkaloids. Solanum plants differ in the content and composition of glycoalkaloids in organs. In wild and cultivated potato species, more than 50 steroidal glycoalkaloids were recognized. Steroidal glycoalkaloids are recognized as potential allelopathic/phytotoxic compounds that may modify the growth of target plants. There are limited data on the impact of the composition of glycoalkaloids on their phytotoxic potential. Results The presence of α-solasonine and α-solamargine in potato leaf extracts corresponded to the high phytotoxic potential of the extracts. Among the differentially expressed genes between potato leaf bulks with high and low phytotoxic potential, the most upregulated transcripts in sample of high phytotoxic potential were anthocyanin 5-aromatic acyltransferase-like and subtilisin-like protease SBT1.7-transcript variant X2. The most downregulated genes were carbonic anhydrase chloroplastic-like and miraculin-like. An analysis of differentially expressed proteins revealed that the most abundant group of proteins were those related to stress and defence, including glucan endo-1,3-beta-glucosidase acidic isoform, whose expression level was 47.96× higher in potato leaf extract with low phytotoxic. Conclusions The phytotoxic potential of potato leaf extract possessing low glycoalkaloid content is determined by the specific composition of these compounds in leaf extract, where α-solasonine and α-solamargine may play significant roles. Differentially expressed gene and protein profiles did not correspond to the glycoalkaloid biosynthesis pathway in the expression of phytotoxic potential. We cannot exclude the possibility that the phytotoxic potential is influenced by other compounds that act antagonistically or may diminish the glycoalkaloids effect.

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“…Potato is an important vegetable crop and factory raw material. 1 SGAs in germinated potato tubers cause poisoning in human beings and animals when the SGA content exceeds safety standards. However, it is also used as a medicine to treat a variety of diseases.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Therefore, potato is considered as one of the most important food crops globally following wheat, rice, and maize. 1 However, in germinated potato tubers, there is a great amount of toxic steroidal glycoalkaloids (SGAs). Excessive SGAs usually lead to poisoning effects on humans and animals, resulting in severe economic loss.…”

Section: ■ Introductionmentioning

confidence: 99%

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Multiomic Analysis Reveals Core Regulatory Mechanisms underlying Steroidal Glycoalkaloid Metabolism in Potato Tubers

Shen

Hua

Huang

et al. 2021

J. Agric. Food Chem.

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Steroidal glycoalkaloids (SGAs) present in germinated potato tubers are toxic; however, the mechanisms underlying SGA metabolism are poorly understood. Therefore, integrated transcriptome, metabolome, and hormone analyses were performed in this study to identify and characterize the key regulatory genes, metabolites, and phytohormones related to glycoalkaloid regulation. Based on transcriptome sequencing of bud eyes of germinated and dormant potato tubers, a total of 6260 differentially expressed genes were identified, which were mainly responsible for phytohormone signal transduction, carbohydrate metabolism, and secondary metabolite biosynthesis. Two TCP14 genes were identified as the core transcription factors that potentially regulate SGA synthesis. Metabolite analysis indicated that 149 significantly different metabolites were detected, and they were enriched in metabolic and biosynthetic pathways of secondary metabolites. In these pathways, the α-solanine content was increased and the expression of genes related to glycoalkaloid biosynthesis was upregulated. Levels of gibberellin and jasmonic acid were increased, whereas that of abscisic acid was decreased. This study lays a foundation for investigating the biosynthesis and regulation of SGAs and provides the reference for the production and consumption of potato tubers.

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Inheritance of steroidal glycoalkaloids in potato tuber flesh

Cited by 10 publications

References 28 publications

A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Transcriptional and proteomic insights into phytotoxic activity of interspecific potato hybrids with low glycoalkaloid contents

Multiomic Analysis Reveals Core Regulatory Mechanisms underlying Steroidal Glycoalkaloid Metabolism in Potato Tubers

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