A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Ma, Ling; Zhang, Chengqi; Zhang, Bo; Tang, Feng; Li, Futing; Liao, Qinggang; Tang, Dié; Peng, Zhen; Jia, Yuxin; Gao, Meng; Guo, Han; Zhang, Jinzhe; Luo, Xuming; Yang, Haisong; Gao, Dongli; Lucas, William J.; Li, Canhui; Huang, Sanwen; Shang, Yi

doi:10.1038/s41467-021-24266-7

Cited by 53 publications

(60 citation statements)

References 31 publications

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“…Furthermore, in pummelo, the S-haplotypes of the progeny from the self-pollinated ‘GY’, whose S-haplotype was S1S2, segregated into only two classes (S1S2:S2S2) at a distorted ratio of 274:256. This ratio was consistent with an abnormity linked to the S2 locus [ 19 ]. Interestingly, we also found the phenomenon of S-haplotype abnormal separation in cabbage and developed two sets of specific primers to solve the problem of loss of S-haplotypes.…”

Section: Introductionsupporting

confidence: 82%

An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage

Chen

Zhang

Ren

et al. 2022

Plants

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Cabbage (Brassica oleracea L. var. capitata) self-incompatibility is important for heterosis. However, the seed production of elite hybrid cannot be facilitated by honey bees due to the cross-incompatibility of the two parents. In this study, the self-compatibility of 58 winter cabbage inbred lines was identified by open-flower self-pollination (OS) and molecular techniques. Based on the NCBI database, a new class I S-haplotype-specific marker, PKC6F/PKC6R, was developed. Verification analyses revealed 9 different S-haplotypes in the 58 cabbage inbred lines; of these lines, 46 and 12 belonged to class I (S6, S7, S12, S14, S33, S45, S51, S68) and class II (S15) S-haplotypes, respectively. The coincidence rate between the self-compatibility index and S-haplotype was 91%. This study developed a Tri-Primer-PCR amplification method to rapidly select plants with specific S-haplotypes in biased segregated S-locus populations. Furthermore, it established an S-haplotype identification system based on these nine S-haplotypes. To overcome parental cross-incompatibility (18-503 and 18-512), an inbred line (18-2169) with the S15 haplotype was selected from the sister lines of self-incompatible 18-512 (S68, class I S-haplotype). The inbred line (18-2169) showed self-compatibility and cross-compatibility with 18-503. This study provides guidance for self-compatibility breeding in cabbage and predicts parental cross-incompatibility in elite combinations.

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Section: Introductionsupporting

confidence: 82%

An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage

Chen

Zhang

Ren

et al. 2022

Plants

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“…SFB specifically degrades non-self S-RNase through the formation of SCF SFB complex with SCF, while its self S-RNase is not degraded. This inhibits the growth of self-pollen tubes by degrading ribosomal RNA (rRNA), thus presenting self-incompatibility in potato and other plants [ 58 ]. In addition, StKFB08 , StKFB13 , StKFB20 , StKFB22 , StKFB28 , StKFB33 , StKFB35 and StKFB36 , were also highly expressed in stamen or other flower tissues, indicating that they may also regulate potato flowering development.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolutionary and functional analyses of KFB family members in potato

Tang

Dong

et al. 2022

BMC Plant Biol

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Background Kelch repeat F-box (KFB) proteins play vital roles in the regulation of multitudinous biochemical and physiological processes in plants, including growth and development, stress response and secondary metabolism. Multiple KFBs have been characterized in various plant species, but the family members and functions have not been systematically identified and analyzed in potato. Results Genome and transcriptome analyses of StKFB gene family were conducted to dissect the structure, evolution and function of the StKFBs in Solanum tuberosum L. Totally, 44 StKFB members were identified and were classified into 5 groups. The chromosomal localization analysis showed that the 44 StKFB genes were located on 12 chromosomes of potato. Among these genes, two pairs of genes (StKFB15/16 and StKFB40/41) were predicted to be tandemly duplicated genes, and one pair of genes (StKFB15/29) was segmentally duplicated genes. The syntenic analysis showed that the KFBs in potato were closely related to the KFBs in tomato and pepper. Expression profiles of the StKFBs in 13 different tissues and in potato plants with different treatments uncovered distinct spatial expression patterns of these genes and their potential roles in response to various stresses, respectively. Multiple StKFB genes were differentially expressed in yellow- (cultivar ‘Jin-16’), red- (cultivar ‘Red rose-2’) and purple-fleshed (cultivar ‘Xisen-8’) potato tubers, suggesting that they may play important roles in the regulation of anthocyanin biosynthesis in potato. Conclusions This study reports the structure, evolution and expression characteristics of the KFB family in potato. These findings pave the way for further investigation of functional mechanisms of StKFBs, and also provide candidate genes for potato genetic improvement.

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“…Whereas in self-pollination, self S-RNases evade degradation and exert cytotoxicity inside the pollen tube to inhibit its growth (Hua and Kao, 2008;McClure et al, 2011). A neo-functionalization of an F-box gene was reported in two recent studies to underly the Sli locus, responsible for breaking down the stylar incompatible response in potato (Solanum tuberosum L.) (Eggers et al, 2021;Ma et al, 2021). Similarly, an F-box gene represents an interesting candidate with a putative role in the complex downstream pathway that leads to rejection of self-pollen in grasses and a disruptive mutation might lead to SC by halting its inhibiting function.…”

Section: Discussionmentioning

confidence: 99%

Identification of Candidate Genes for Self-Compatibility in Perennial Ryegrass (Lolium perenne L.)

et al. 2021

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Self-incompatibility (SI) is a genetic mechanism preventing self-pollination in ~40% of plant species. Two multiallelic loci, called S and Z, control the gametophytic SI system of the grass family (Poaceae), which contains all major forage grasses. Loci independent from S and Z have been reported to disrupt SI and lead to self-compatibility (SC). A locus causing SC in perennial ryegrass (Lolium perenne L.) was previously mapped on linkage group (LG) 5 in an F2 population segregating for SC. Using a subset of the same population (n = 68), we first performed low-resolution quantitative trait locus (QTL) mapping to exclude the presence of additional, previously undetected contributors to SC. The previously reported QTL on LG 5 explained 38.4% of the phenotypic variation, and no significant contribution from other genomic regions was found. This was verified by the presence of significantly distorted markers in the region overlapping with the QTL. Second, we fine mapped the QTL to 0.26 centimorgan (cM) using additional 2,056 plants and 23 novel sequence-based markers. Using Italian ryegrass (Lolium multiflorum Lam.) genome assembly as a reference, the markers flanking SC were estimated to span a ~3 Mb region encoding for 57 predicted genes. Among these, seven genes were proposed as relevant candidate genes based on their annotation and function described in previous studies. Our study is a step forward to identify SC genes in forage grasses and provides diagnostic markers for marker-assisted introgression of SC into elite germplasm.

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A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

Cited by 53 publications

References 31 publications

An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage

An Identification System Targeting the SRK Gene for Selecting S-Haplotypes and Self-Compatible Lines in Cabbage

Genome-wide identification, evolutionary and functional analyses of KFB family members in potato

Identification of Candidate Genes for Self-Compatibility in Perennial Ryegrass (Lolium perenne L.)

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