Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.)

Nutritionally Enhanced Staple Food Crops

“…This latter QTL was linked with a QTL found for Mesoamerican beans by Blair et al (2010c). Further studies by Blair et al (2009bBlair et al ( , 2010b in both inter-and intragene pool populations, respectively, found specific major and minor QTL for Fe and Zn concentrations with the former type mainly on LG b11. Therefore, at least four major QTL have been identified in common bean affecting micronutrient concentration depending on the gene pool and genetic background of the material tested.…”

“…LGs, 17%-18% variation; a QTL for P (19% variation) on B1 colocated with QTLs accounting 34% variation each for Fe and Zn on same LG Cichy et al 2009 Six QTL for P on LG B2 and B6 and three QTL for Phyt on LG B6, with QTL for P or Phyt were related to seed weight QTL on LGs B6, B7, and B10 in an inter-gene pool mapping population Blair et al 2009b Rice Mutant alleles of the low phyt homozygous lethal (XS-lpa2-1) and nonlethal (XS-lpa-2-2) mapped: XS-lpa2-1 gene to a region on chromosome 3 between marker RM14360 and RM1332, where also located the rice orthologue (OsMRP5) of the maize lpa1; a single base pair change in the sixth exon of XS-lpa2-1 and a 5-bp deletion in the first exon of XS-lpa2-2 resulted these mutations Xu et al 2009 TIGR locus LOC_Os02g57400 identified as the candidate gene for mutant alleles: lpa1-1 is a single base pair substitution mutation while lpa1-2 involves a 1,475 bp fragment deletion; a CAPS marker (LPA1_CAPS) for lpa1-1…”

“…The atomic absorption spectrophotometer (AAS) method has been suggested for routine estimation of Fe and Zn in seeds (Sahrawat et al 2002;Blair et al 2009bBlair et al , 2010aBlair et al ,b,2011). An excellent review on the role of atomic spectrometry in plant science has been published elsewhere, and it is important to recognize recent developments in multielemental and speciation analyses in plants with the resulting functional roles of different elements in plant science ).…”

“…X-ray fluorescence spectrometry is another method that allows identifying a wide range of micronutrients including elements such as P, which is indicative of the antinutrient phytate. Inductively coupled plasma (ICP) analysis is more of a gold standard but is expensive and therefore of less utility in breeding especially as it is usually highly correlated with AAS readings (Blair et al 2009b(Blair et al , 2010c and is the current method of choice to detect elements such as aluminum, which has been proposed as an indicator of iron contamination (Stangoulis and Sison 2008). Lorenz et al (2007) developed a rapid and inexpensive method for measuring phytate and inorganic phosphorus (Pi) concentrations in maize, which provides adequate precision and simplicity to deal with large number of breeder's samples for estimating phytate and Pi levels simultaneously.…”

Sequence related amplified polymorphism (SRAP) analysis for genetic diversity and micronutrient content among gene pools in mungbean [Vigna radiata (L.) Wilczek]

“…In our study, the value of correlation coefficient (r) was found to be 0.434. The inheritance of iron and zinc accumulation in common bean was shown to be predominantly quantitative with some common QTL found for both minerals (Blair et al 2009). Colocalizing QTL was most notable on linkage group B11 for seed iron and zinc.…”