Inheritance of RAPD fragments in haploid and diploid tissues of Pinus sylvestris (L.)

Abstract

We studied the inheritance of RAPD fragments in haploid and diploid tissues of eight parents and 80 diploid controlled F1 progeny of Pinus sylvestris (L.). Most fragments detected in the diploid tissue of parents were also present in their haploid macrogametophytes. All fragments observed in the parents were found in the progeny. Individual fragments amplified by a single primer were observed in unrelated trees suggesting that primer competition is not likely to cause 'epistatic' effects on RAPD polymorphism. Segregation of all variable fragments observed in haploid and diploid materials fitted the proportions expected for a dominant Mendelian trait. The segregating fragments were either present or absent confirming the dominant character of RAPD variation reported in most previous studies. None of the fragments detected in this study appeared to originate from uniparentally inherited cytoplasmic DNA. Our present results provide additional support for the usefulness of RAPD fragments as genetic markers. Nevertheless, the few differences observed between haploid and diploid tissues with respect to some fragments emphasize the need for inheritance studies preceding the use of RAPD fragments as genetic markers.

Identification of the Male Parents of Half-sib Progeny from Japanese Black Pine (Pinus thunbergii Parl.) Clonal Seed Orchard using RAPD Markers.

“…Use of RAPD markers for the parentage analysis has been limited (Welsh et al 1991, Scott et al 1992, Akerman et al 1995, because of their dominant character. However, the limitation of the dominant nature of RAPD markers can be alleviated by the segregation analysis of haploid megagametophytes (Lu et al 1995, Szmidt et al 1996. We were able to identify the male parents of most seedlings from the diagnostic progeny, by comparing the genotype of orchard clones inferred by analyzing Fig.…”

“…Nevertheless, it is noteworthy that we can show the possibility of RAPD markers for the paternity analysis in seed orchards. RAPD markers are very polymorphic in most conifers (Carlson et al 1991, Lu et al 1995, Van de Ven and McNicol 1995, Goto 1998, and it is possible to find RAPD markers sufficient for the paternity analysis. Furthermore RAPD markers are already being used in most laboratories, and no prior sequence information is needed before starting the research.…”

“…Recently, inheritance of RAPD fragments in F 1 generations and haploid tissues has been reported (Carlson et al 1991, Roy et al 1992, Lu et al 1995. It has been confirmed that they segregate as a Mendelian trait in most cases.…”

“…It has been confirmed that they segregate as a Mendelian trait in most cases. Lu et al (1995) succeeded in deducing parent genotypes by segregation analysis of several haploid megagametophytes. In order to utilize the valuable feature that conifers have both haploid and diploid tissues, it is necessary to ascertain that individual RAPDs can be detected in both types of tissue (Lu et al 1995).…”

“…Lu et al (1995) succeeded in deducing parent genotypes by segregation analysis of several haploid megagametophytes. In order to utilize the valuable feature that conifers have both haploid and diploid tissues, it is necessary to ascertain that individual RAPDs can be detected in both types of tissue (Lu et al 1995). In this respect, clones of conifer seed orchards are particularly well suited for identifying the genotype using RAPD fragments.…”