Inheritance of powdery mildew (Erysiphe polygoni DC) resistance in mungbean (Vigna radiata L. Wilczek)

Abstract

Detached mungbean (Vigna radiata L.Wilczek) leaves were inoculated with a conidial suspension of a local isolate (TI-1) of the powdery mildew pathogen (Erysiphe polygoni DC) under controlled environment conditions. Based on the latent period and severity of the infection, a rating scale of 0-5 was used to classify the host pathogen interactions. Reactions 0, 1 and 2 were considered resistant and referred to as R0, R1 and R2 while 3, 4 and 5 were classified as susceptible (S). RUM lines (resistant to powdery mildew) and their derivatives are crossed with several susceptible (reaction types 3-5) genotypes and the inheritance of the resistance was studied in the F1, F2 and F3 generations. The results showed that powdery mildew resistance in mungbean is governed by two dominant genes designated as Pm-1 and Pm-2. When both Pm-1 and Pm-2 were present, an R0 reaction was observed after inoculation with TI-1. The resistant reaction was R1 when only Pm-1 was present and R2 in the presence of Pm-2. In the absence of both Pm-1 and Pm-2, susceptible reactions 3, 4 and 5 were observed.

Perspectives and Challenges for Sustainable Management of Fungal Diseases of Mungbean [Vigna radiata (L.) R. Wilczek var. radiata]: A Review

“…Mungbean germplasm accessions can be screened by inoculating with the pathogen in the controlled environments. Reliable and efficient methods were developed for screening mungbean seedlings against powdery mildew in the greenhouse (Wongpiyasatid et al, 1999;Kasettranan et al, 2010); and in the laboratory using a detached leaf assay (Reddy et al, 1987). For the assessment of foliar diseases, both qualitative and quantitative rating scales were used (Reddy et al, 1994b;Wongpiyasatid et al, 1999;Khunti et al, 2005;Marappa, 2008;Suryawanshi et al, 2009).…”

“…However, identification of molecular markers associated with disease resistant major genes and QTL shows the potential application of MAS in mungbean disease resistance. Genetic studies using different sources of resistance revealed both monogenic (qualitative) and quantitative modes of inheritance in mungbean for powdery mildew resistance (Reddy et al, 1994a;Kasettranan et al, 2009). Gawande and Patil (2003) reported that both additive and dominant gene actions were important in inheritance of powdery mildew resistance including non-allelic interactions.…”

“…Gawande and Patil (2003) reported that both additive and dominant gene actions were important in inheritance of powdery mildew resistance including non-allelic interactions. Several earlier studies reported monogenic inheritance of powdery mildew resistance controlled by single dominant genes and studies were conducted using mungbean varieties ML3 and ML5 (AVRDC, 1979), and breeding lines VC 1560A (AVRDC, 1981), ATF 3640 (Humphry et al, 2003) and RUM (Reddy et al, 1994a). Using restriction fragment length polymorphism (RFLP) markers, Chaitieng et al (2002) (Young et al, 1993;Sorajjapinun et al, 2005).…”

Genotypes against Major Diseases in Green Gram and Black Gram under Natural Field Conditions

“…Similarly Jameel Akhtar et al, (2014) Several sources of resistance to Powdery mildew disease in mungbean have been reported (Hartman et al, 1993 andReddy et al, 1994b). Genetic studies using different resistance sources revealed different modes of inheritance (Reddy et al, 1994a;Reddy, 2009;Sorajjapinun et al, 2005 andKasettranan et al, 2009), suggesting that there are different mechanisms or genes conferring resistance to powdery mildew disease.…”

“…Numerous attempts have been made for the identification of resistant sources against these diseases (Basandrai et al, 1999(Basandrai et al, , 2003(Basandrai et al, , 2011aIqbal et al, 2004;Raje and Rao, 2002;Reddy et al, 1994aReddy et al, , 2001Reddy et al, and 2008 of Green gram and Black gram.…”