The Selective Silylation of D-Mannitol Assisted by Phenylboronic Acid and the Solid State and Solution Structures of the Intermediate 1,6-Bis(silyl) Bis(phenylboronates). -Condensation of D-mannitol (I) with two equivalents of phenylboronic acid followed by silylation with t-butylsilyl chlorides (III) produces the unusually stable 1,6-disilyl bis(boronates) (IV), which require oxidative conditions to cleave the boronate esters. X-ray crystal structure analyses of the intermediates (IV) reveal that they exist as two fused six-membered rings. NMR studies suggest that these structures also predominate in solution. -(BHASKAR, V.; D'ELIA, A.; DUGGAN*, P. J.; HUMPHREY, D. G.; KRIPPNER, G. Y.; NHAN, T. T.; TYNDALL, E. M.; J.
Cyclodextrins (CDs) are carrier molecules produced by cyclization of α-1,4-glucans by Cyclodextrin Glycosyl Transferase (CGTase). These torus shaped molecules have hydrophobic cavity and hydrophilic shell making them useful in pharmaceutical, food, textile, pesticide and cosmetic industries. In this study, culture conditions for the production of CGTase by organism belonging to Arthrobacter genus obtained from a paddy field soil were optimized by single parameter mode. Soluble starch, yeast extract and magnesium sulphate played an important role in CGTase production. Percentage increase in CGTase yield under optimized conditions was 396.77%. The enzyme precipitated by 60% ammonium sulphate was purified using DEAE-sepharose. The molecular weight of the purified protein as determined by SDS-PAGE was 75 kDa. Purified CGTase was thermostable and stable over a wide pH range. Dissolution studies on β -cyclodextrin-Irbesartan complex revealed that β -CDs formed were useful in preparing immediate release oral dosage forms.
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