Inheritance of male sterility mutations induced in haploid sorghum tissue culture

Elkonin, L. A.; Gudova, T. N.; Ishin, A. G.

doi:10.1007/bf00039305

Cited by 10 publications

(6 citation statements)

References 15 publications

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“…It was found that young anthers produced more callus as compared to mature anthers. A number of reports are available on studies concerning haploid sorghum culture and culture of male sterile lines (Rose et al, 1986a, b;Elkonin et al, 1987Elkonin et al, , 1993Elkonin et al, , 1994Elkonin et al, , 1995Elkonin, 1995;Elkonin andEnaleeva, 1997, 1998). Cytological analysis of meiosis in sterile plants by Elkonin and Enaleeva (1998) revealed that the tissue culture-induced mutation caused significant abnormalities in chromosome synapsis during prophase-I that resulted in the formation of multiple micronuclei during cell division.…”

Section: In Vitro Plant Regeneration Haploidy Polyploidy and Somaclmentioning

confidence: 96%

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

Maqbool

Devi

Sticklen

2001

In Vitro Cell.Dev.Biol.-Plant

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This report reviews the contributions to the improvement of sorghum (Sorghum bicolor (L.) Moench) through traditional approaches with emphasis on the application of biotechnological methods. Strategies include breeding for higher yield, improved grain quality, and biotic and abiotic stress tolerance. Hybrid development and polyploidy breeding are also discussed. Plant breeders, working in concert with biotechnologists, have developed new powerful tools for plant genetic manipulation and genotype evaluation that will significantly improve the efficiency of plant breeding. Improving sorghum through biotechnology is the latest in a long series of technologies that have been applied to this crop. Five basic tools of technology have been developed for sorghum improvement: (1) in vitro protocols for efficient plant regeneration; (2) molecular markers; (3) gene identification and cloning; (4) genetic engineering and gene transfer technology to integrate desirable traits into the sorghum genome; and (5) genomics and germplasm databases. Reports on studies involving the problems, progress, and prospects for utilizing the biotechnological methods for sorghum improvement are discussed.

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Section: In Vitro Plant Regeneration Haploidy Polyploidy and Somaclmentioning

confidence: 96%

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

Maqbool

Devi

Sticklen

2001

In Vitro Cell.Dev.Biol.-Plant

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“…Panicles of 1-2 tillers per plants were covered using paper bag to avoid any cross pollination that might occur. At seed setting stage, number of seeds set per spikelets was counted among the R0 regenerants and fertility status of the genotypes was estimated following Elkonin et al (1994) method. Male sterile plants were recognized with the production of anthers without pollen grains dehisced on fresh receptive stigmas.…”

Section: Fertility Investigationmentioning

confidence: 99%

Male sterility and gametoclonal variations from gynogenically derived polyploids of tef (Eragrostis tef), zucc. Trotter

Gugsa¹,

Loerz²

2013

Afr. J. Plant Sci.

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The production of plants from the gynogenic culture of tef (Eragrostis tef) was reported from in vitro gynogenesis of unpollinated flower explant of cultivar 'DZ-01-196'. Out of the 159 regenerated R0 lines, 151 tetraploids, 5 di-haploids, 2 aneuploids and 1 octoploid plant were obtained which showed gametoclonal variation in various traits including male sterility. Panicle bending, accessory floret development and larger seed size were among the unique traits obtained. Analysis of variance revealed significant differences for all morphological traits studied in the R0 generations. Tetraploid R0s showed superiority over the control for plant height, flag leaf length and number of florets per spikelet. Male sterility traits were recorded among the tetraploid R0 lines mainly due to failure of pollen formation. Though two complete and four partial male sterile lines were obtained in the R0 generation, this trait was not exhibited in those plants raised from the partially male sterile lines. Male sterility and the various gametoclonal variations obtained from this study will facilitate the hybridization and crop improvement program of tef.

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“…Previously, we have obtained male-sterile mutants from tissue culture of sorghum (Elkonin et al 1994). A significant influence of nuclear background on expression of induced male sterility was observed.…”

Section: Introductionmentioning

confidence: 99%

Cytological investigation of male sterility in sorghum caused by a dominant mutation (Ms tc ) derived from tissue culture

Tsvetova

Elkonin

2003

Sex Plant Reprod

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Male sterility mutations are an important tool in the investigation of anther and pollen development and for obtaining hybrid seeds in plant breeding. Cytological analysis of microsporo-and microgameto-genesis in sorghum plants with dominant mutation of male sterility (Ms tc ) derived from tissue culture has been carried out. Using substitution backcrosses, this mutation was introduced first into the nuclear background of the fertile sorghum line SK-723 and from this line into Volzhskoe-4w (V-4w). The mechanism of Ms tc action on anther and pollen development differed in different nuclear backgrounds. In SK-723, phenotypic expression of Ms tc began before the beginning of meiosis, which resulted in degeneration of sporogenous tissue in some anthers and in significant disturbances of anther morphology. In microsporocytes that did not degenerate, the frequency of non-specific meiotic abnormalities characteristic of the fertile line SK-723 significantly increased. In addition, in the mutant plants, a number of specific meiotic abnormalities-almost complete desynapsis, and formation of syncytial structures-were observed, apparently the consequence of Ms tc action. In mono-or bi-nucleate microspores, degenerative processes resulting in formation of empty or anomalously coloured pollen grains led to almost complete male sterility. In the V-4w nuclear background, changes in anther structure and meiotic disturbances were infrequent. The degenerative processes began at the uni-or binucleate microspore stage and resulted in formation of empty or abnormally coloured pollen grains, and in partial pollen sterility. Thus, the same nuclear male sterility-inducing mutation in different nuclear backgrounds affects different stages of pollen development.

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Inheritance of male sterility mutations induced in haploid sorghum tissue culture

Cited by 10 publications

References 15 publications

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

Biotechnology: Genetic improvement of sorghum (Sorghum bicolor (L.) Moench)

Male sterility and gametoclonal variations from gynogenically derived polyploids of tef (Eragrostis tef), zucc. Trotter

Cytological investigation of male sterility in sorghum caused by a dominant mutation (Ms tc ) derived from tissue culture

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