Inheritance of high palmitic acid content in the seed oil of sunflower mutant CAS-5

Pérez‐Vich, Begoña; Fernandez, J. A.; Garcés, Rafael; Fernández‐Martínez, José M.

doi:10.1007/s001220051097

Cited by 26 publications

(10 citation statements)

References 16 publications

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“…Accordingly, the alleles ln2 will not have a phenotypic effect for reduced linolenic-acid concentration in C-101, which presents a standard fatty acid profile, thus being feasible different allelic configurations in lines derived from it such as N2-3591 and N2-4961 (Velasco et al 1997a). Similar models have been postulated for genes controlling increased levels of saturated fatty acids in sunflower (PØrez-Vich et al 1999a, b). According to this hypothesis, the genetic constitution of the three parents was as follows: LnLnLn1Ln1Ln2Ln2 for N2-3591, lnlnLn1Ln1ln2ln2 for N2-4961, and LnLnln1ln1ln2ln2 for HF-186.…”

Section: Discussionmentioning

confidence: 85%

Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm

et al. 2003

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Seed oil of current zero erucic-acid germplasm of Ethiopian mustard ( Brassica carinata A. Braun) is characterized by a low concentration of oleic acid and high concentrations of linoleic and linolenic acids. Sources of increased oleic-acid (HO) and reduced linolenic-acid (LL) concentration have been developed separately in high erucic-acid germplasm. The objectives of the present research were to study the inheritance of the HO and LL traits in crosses HO x LL, and to develop HOLL recombinants, both in high erucic-acid and zero erucic-acid backgrounds. The HO mutant N2-3591 (about 20% oleic acid compared to 9% in conventional high erucic-acid materials), was reciprocally crossed with the LL lines N2-4961 and HF-186 (both with about 5% linolenic acid compared to 12% in standard high erucic-acid materials). Increased oleic acid concentration of N2-3591 was found to be controlled by alleles at one locus (Ol), whereas three different loci for reduced linolenic-acid concentration (Ln, Ln1 and Ln2) were identified in N2-4961 and HF-186. Crosses between N2-3591 and N2-4961 generated HOLL recombinants where levels of increased oleic-acid and reduced linolenic-acid were similar to those of the parents. However, a transgressive segregation for oleic acid was observed in crosses between N2-3591 and HF-186, where F(2) seeds with up to 29.7% oleic acid were obtained, in comparison to an upper limit of 25.1% in the N2-3591 parent grown in the same environment. The transgressive increased oleic-acid was expressed in the F(3) generation and was attributed to the presence of a second locus, designated Ol2. The transgressive trait was transferred to the zero erucic-acid line 25X-1, resulting in a zero erucic-acid germplasm with very high oleic-acid concentration (83.9% compared to 32.9% in 25X-1) and low linolenic-acid concentration (5.0% compared to 16% in 25X-1). Additionally, two other lines exhibiting different stable levels of increased oleic-acid (70.7% and 79.5%, respectively) and reduced levels of linolenic-acid (7.5% and 8.7%, respectively) were isolated.

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Section: Discussionmentioning

confidence: 85%

Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm

et al. 2003

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“…Mutagenic breeding programs aimed at reducing the concentration of polyunsaturated fatty acids have been reported in several oilseeds such as soybean, sunflower and flax. Selections from these programs have been used extensively to study the inheritance of fatty acid composition and to develop new cultivars with specif ic fatty acid prof iles (Ntiamoah et al, 1995;Rahman et al, 1996;Pérez-Vich et al, 1999). Conversely, breeding programs in palm tree aimed at generating more liquid oils have led to individuals with high percentages of unsaturated fatty acids (Jones, 1984).…”

Section: Introductionmentioning

confidence: 99%

Variability of fatty acid composition in olive (Olea europaea L.) progenies

León

Uceda

Jiménez³

et al. 2004

Span. j. agric. res.

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Seedlings from crosses between the olive cultivars "Arbequina", "Frantoio" and "Picual" were evaluated for fatty acid composition over two consecutive years. Gas chromatography was used for analyzing the main fatty acids of olive oil: palmitic (C16:0), palmitoleic (C16:1), stearic (C18:0), oleic (C18:1) and linoleic (C18:2). Wide ranges of variation were obtained for all the fatty acids; as large or even larger than the ranges reported from the evaluation of olive cultivar collections. Significant differences were found between crosses for all the fatty acids analysed except for palmitic acid and between years for all of them except stearic acid, being the interaction crosses x year non significant for any of them. The wide variability observed for all the fatty acids represents a very promising base to obtain new olive cultivars with high oil quality, as vegetative propagation allows the conservation of the most interesting plants.

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“…Furthermore, the CAS-5 mutant also accumulated palmitolinoleic acid (16:2n-4). At least three partially recessive genes are responsible for the production of high quantities of palmitic FA in these mutants (4). Biochemical characterization of these mutants showed that the keto-acyl synthase II (KASII), an enzyme of the FAS II complex, was less active than the standard sunflower KASII.…”

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Lipid characterization of seed oils from high‐palmitic, low‐palmitoleic, and very high‐stearic acid sunflower lines

Serrano-Vega

Martı́nez-Force

Garcés

2005

Lipids

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Information obtained in recent years regarding the enzymes involved in FA synthesis can now be applied to develop novel sunflower lines by incorporating enzymes with specific characteristics into lines with a defined background. We have generated three highly saturated mutant lines in this way and characterized their FA content. The new high-palmitic, low-palmitoleic lines CAS-18 and CAS-25, the latter on a high-oleic background, have been selected from the high-stearic mutant CAS-3 by introducing a deficient stearic acid desaturase in a high-palmitic background from the previously developed mutant lines CAS-5 and CAS-12, respectively. As such, the desaturation of palmitic acid and the synthesis of palmitoleic acid and its derivatives (asclepic and palmitolinoleic acids) were reduced in these high-palmitic lines, increasing the stearic acid content. Likewise, introducing a FA thioesterase from a high-palmitic line (e.g., CAS-5) into the high-stearic CAS-3 increased the stearic acid content from 27 to 32% in the new high-stearic line CAS-31. As previously described in high-palmitic lines, high growth temperatures did not reduce the linoleic acid content of the oil. Furthermore, the FA composition of TAG, DAG, and phospholipids was modified in these lines. Besides a high degree of saturation, the TAG from these new vegetable oils have a low content of saturated FA in the sn-2 position. The alpha asymmetric coefficient obtained also indicates that the saturated FA are asymmetrically distributed within the TAG molecules. Indeed, the disaturated TAG content rose from 31.8 to 48.2%. These values of disaturated TAG are the highest to date in a temperate oilseed.

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Inheritance of high palmitic acid content in the seed oil of sunflower mutant CAS-5

Cited by 26 publications

References 16 publications

Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm

Development of high-oleic, low-linolenic acid Ethiopian-mustard (Brassica carinata) germplasm

Variability of fatty acid composition in olive (Olea europaea L.) progenies

Lipid characterization of seed oils from high‐palmitic, low‐palmitoleic, and very high‐stearic acid sunflower lines

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