Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment

Abstract

Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.

GENETIC TRANSFORMATION AND REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) USING Agrobacterium SYSTEM

“…Apical meristems of seedlings were submitted to particle bombardment, and after stimulation of axillary shoot proliferation, transgenes plants were recovered at 0.03% of shoots. Using the same strategy, Aragão et al (1996) obtained transformants of cv. Olathe.…”

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate

“…Despite its importance, production growth rates are the number of shoots and leaves differed significantly limited by viral, fungal and bacterial pathogens, insects, among the common bean cultivars evaluated. The lack of drought tolerances and nutritional deficiencies higher average of shoots was obtained for Brunca > (Aragão et al 1996). Therefore, there is considerable Telire > Bribrí > Guaymí > Huetar.…”

“…Therefore, there is considerable Telire > Bribrí > Guaymí > Huetar. Moreover, interest in the development of new bean cultivars with independently of the cultivar, the induction medium useful agronomical traits (Aragão et al 1996). supplemented with 5 mgl -1 BAP and 20 or 40 mgl -1 AS resulted in the higher average of shoots formation.…”

The challenge of vector development in gene therapy

“…Conceptually the most obvious of such methods, direct injection, requires sophisticated techniques for injection on a micro-scale. originally used in gene transfer to plant cells and tissues (9) and then applied to a variety of organisms including mammal cells and tissues (10)(11)(12)(13)(14)(15)(16) consists of coating tiny spheres of a heavy metal (e.g., gold) with DNA, and then shooting these coated particles against the target cells at high speed. The accelerated particles penetrate the cells carrying the DNA.…”