Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene

Abstract

The most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F 2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F 2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT 300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs.

Marcadores moleculares derivados de sequências expressas do genoma café potencialmente envolvidas na resistência à ferrugem

“…Com a ferramenta "electronic northern" (Carazzolle et al, 2007), foram agrupadas as 214.964 ESTs provenientes das 37 bibliotecas de cDNA do CafEST (Vieira et al, 2006). A percentagem normalizada de ESTs das bibliotecas que constituem cada um dos "contigs" foi descrita.…”

“…Os iniciadores que apresentaram perfil de bandas monomórficas em agarose foram posteriormente submetidos à eletroforese em géis desnaturantes de poliacrilamida a 6%, os quais foram corados com nitrato de prata a 4% e visualizados em transiluminador sob luz branca. As imagens obtidas foram digitalizadas de acordo com Brito et al (2010).…”

Phenylalanine Ammonia-Lyase and Source-Flow-Sink Related Attributes in Rice Genotypes Subjected to High Night Temperatures

“…Abiotic and biotic stresses represent the major constraints that result in agricultural losses on the global scale and projected climate changes could increase their negative effects in the future (Brito et al, 2010(Brito et al, , 2016Diola et al, 2011Diola et al, , 2013Weber et al, 2014;Guimarães et al, 2017). Recently, reports are highlighting via climate models predict an increasing frequency and intensity of heat waves and high temperature events throughout current century (IPCC, 2013;Becklin et al, 2016).…”

Field Performance and Genetic Fidelity of Micropropagated Plants of Coffea canephora (Pierre ex A. Froehner)

“…Various molecular markers such as RAPD [11][12][13][14], AFLP [15,16], SSR [17] and ISSR [18] are used for determining the genetic fidelity of micropropagated plants. In coffee (C. arabica), Landey et al [19] analyzed genetic fidelity of in vitro propagated plants of C. arabica using AFLP and SSAP markers and confirmed that genetic variation observed in micropropagated plants are caused by abnormal chromosome numbers.…”