Ingredient selection for plastic composite supports for L-(+)-lactic acid biofilm fermentation by Lactobacillus casei subsp. rhamnosus

Ho, Kai-Lai G.; Pometto, Anthony L.; Hinz, Paul N.; Dickson, James S.; Demırcı, Ali

doi:10.1128/aem.63.7.2516-2523.1997

Cited by 61 publications

(41 citation statements)

References 15 publications

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“…At the same time, they are called as microbial cell layers, which are attached on the support materials. Due to high biomass concentration, stability, and potential of long-term fermentation of cells attached on the surface materials, biofilm reactors can be used for production of value-added products such as human lysozyme (Ercan and Demirci 2015b), ethanol (Germec et al 2015(Germec et al , 2016, lactic acid (Ho et al 1997), kojic acid (Liu et al 2016b), isoflavone aglycones (Liu et al 2016a), etc. Nonetheless, microparticles used as a novel approach to improve morphology engineering of filamentous microorganisms and increase their productivity are also applied.…”

Section: Introductionmentioning

confidence: 99%

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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Mannanases, one of the important enzyme group for industry, are produced by numerous filamentous fungi, especially Aspergillus species with different fermentation methods. The aim of this study was to show the best fermentation method of β-mannanase production for fungal growth in fermenter. Therefore, different fermentation strategies in fed-batch fermentation (suspended, immobilized cell, biofilm and microparticle-enhanced bioreactor) were applied for β-mannanase production from glucose medium (GM) and carob extract medium (CEM) by using recombinant Aspergillus sojae. The highest β-mannanase activities were obtained from microparticle-enhanced bioreactor strategy. It was found to be 347.47 U/mL by adding 10 g/L of AlO to GM and 439.13 U/mL by adding 1 g/L of talcum into CEM. The maximum β-mannanase activities for suspended, immobilization, and biofilm reactor remained at 72.55 U/mL in GM, 148.81 U/mL in CEM, and 194.09 U/mL in GM, respectively. The reason for that is the excessive, and irregular shaped growth and bulk formation, inadequate oxygen transfer or substrate diffusion in bioreactor. Consequently, the enzyme activity was significantly enhanced by addition of microparticles compared to other fed-batch fermentation strategies. Also, repeatable β-mannanase activities were obtained by controlling of the cell morphology by adding microparticle inside the fermenter.

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Section: Introductionmentioning

confidence: 99%

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

et al. 2017

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“…Repeated-batch fermentations were performed in a bench stirred tank reactor (Sartorius Biostad B Plus, Goettingen, Germany) with a 5-L vessel (working volume of 3-L) with the selected plastic composite support (PCS) material, which consists of polypropylene (50%, w/w), soybean hull (35%, w/w), soybean flour (5%, w/w), yeast extract (5%, w/w), bovine albumin (5%, w/w), and mineral salts at trace quantity [32]. The prepared inoculum (24-h grown culture of S. cerevisiae) was used to inoculate the reactor at 1% (v/v) ratio [5].…”

Section: Repeated-batch Fermentationsmentioning

confidence: 99%

Kinetic Modeling and Techno-economic Feasibility of Ethanol Production From Carob Extract Based Medium in Biofilm Reactor

et al. 2019

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In this study, different carob extract-based media containing Medium A (included all ingredients), Medium B (included yeast extract and salts), Medium C (included (NH4)2SO4 and salts), Medium D (included only salts) and Medium E (included no ingredients) were evaluated for ethanol fermentation by Saccharomyces cerevisiae in a biofilm reactor and their results were used for kinetic modeling. The logistic model for cell growth, Luedeking-Piret model for ethanol production and Modified Luedeking-Piret model for substrate consumption were studied. Kinetic parameters were determined by fitting the observed values of the models. The findings indicated that the predicted data with the suggested kinetic model for each medium fitted very well the experimental data. Estimated kinetics were also in good agreement with experimental kinetics. The techno-economic analysis was performed with the unit costs of the components used in the medium and ethanol. Medium-based process economic feasibility proved carob extract-based Medium E and subsequently Medium D as most economical for ethanol production. The present study verified the potential of carob extract-based medium for increased economical production of ethanol. In conclusion, the ethanol production in a biofilm reactor is growth-associated since α (gP/gX) was greater than β (gP/gX.h) and Media D and E increased the economic production of carob extract-based ethanol.

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“…PCS disks employed were a blend of 50% (w/w) polypropylene (Quantum USI Division, Cincinnati, OH), 35% (w/ w) ground soybean hulls (20 mesh) and vacuum-dried (48 h at 110°C, 30 in. of mercury) soybean hulls (Cargill Soy Processing Plant, Iowa Falls, IA), 5% (w/w) yeast extract (Ardamine Z, Universal Flavors), 5% (w/w) defatted soybean flour (Archer Daniels Midland, Decatur, IL), 5% (w/ w) bovine albumin (American Protein Corp., Ames, IA), and mineral salts (2 g of sodium acetate, 1.2 g of MgSO 4 и 7H 2 O, and 0.06 g of MnSO 4 и 7H 2 O per kilogram of mixture) (Ho et al, 1997b). PCS was prepared by high-temperature extrusion in a Brabender PL2000 corotating twin-screw extruder (Model CTSE-V, C. W. Brabender Instruments, Inc., South Hackensack, NJ) through a tubing die assembly (3.2-mm i.d.…”

Section: Plastic Composite Supportsmentioning

confidence: 99%

“…and glucose concentration, and viable suspended cell counts. Biofilm on PCS disks was determined by the stripping sand method (Ho et al, 1997b), followed by fluorescent differential stain flow cytometry. LAF medium without solvent was evaluated as control.…”

Section: Biofilm Fermentationmentioning

confidence: 99%

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Resistance of Lactobacillus casei in plastic‐composite‐support biofilm reactors during liquid membrane extraction and optimization of the lactic acid extraction system

Demirci¹,

Cotton²,

Pometto³

et al. 2003

Biotech & Bioengineering

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Lactic acid fermentations were performed with plastic-composite-support (PCS) disks in solvent-saturated media with Lactobacillus casei subsp. rhamnosus (ATCC 11443). The PCS disks contained 50% (w/w) polypropylene, 35% (w/w) ground soybean hulls, 5% (w/w) yeast extract, 5% (w/w) soybean flour, and 5% (w/w) bovine albumin. Bioassays were performed by growing L. casei in solvent-saturated media after soaking the PCS disks. Eighteen different solvent and carrier combinations were evaluated. Overall, L. casei biofilm fermentation demonstrated the same lactic acid production in solvent-saturated medium as suspended cells in medium without solvents (control). To evaluate PCS solvent-detoxifying properties, two bioassays were developed. When solvent-saturated medium in consecutive equal volumes (10 mL then 10 mL) was exposed to PCS, both media demonstrated lactic acid fermentation equal to the control. However, when solvent-saturated medium with two consecutive unequal volumes (10 mL then 90 mL) was exposed to PCS, some degree of toxicity was observed. Furthermore, iso-octane, tributylphosphate (TBP), and Span 80 were optimized for recovery as 91%, 5%, and 4% (v/v), respectively, with a 1:1 ratio of 1.2 M Na(2)CO(3) stripping solution. Also, recovery by emulsion liquid extraction in the hollow-fiber contactor was minimal due to low recovery at pH 5.0 and incompatibility of the solvent and hollow-fiber material. These results suggest that PCS biofilm reactors can benefit lactic acid fermentation by eliminating the toxic effect from solvent leakage into the fermentation medium from liquid-liquid extractive integrated fermentations.

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Ingredient selection for plastic composite supports for L-(+)-lactic acid biofilm fermentation by Lactobacillus casei subsp. rhamnosus

Cited by 61 publications

References 15 publications

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Effect of different fermentation strategies on β-mannanase production in fed-batch bioreactor system

Kinetic Modeling and Techno-economic Feasibility of Ethanol Production From Carob Extract Based Medium in Biofilm Reactor

Resistance of Lactobacillus casei in plastic‐composite‐support biofilm reactors during liquid membrane extraction and optimization of the lactic acid extraction system

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