Ingestible Gastrointestinal Sampling Devices: State-of-the-Art and Future Directions

Amoako-Tuffour, Yaw; Jones, Mitchell L.; Shalabi, Nabil; Labbé, Alain; Vengallatore, Srikar; Prakash, Satya

doi:10.1615/critrevbiomedeng.2014010846

Cited by 22 publications

(20 citation statements)

References 99 publications

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“…Conventional clinical approaches of exploring and collecting GI content remain invasive, resource intensive, and often unable to capture all the information contained in these heterogeneous GI samples. A new class of GI sampling capsules is available, which is based on an intra-luminal technique that offers the possibilities of the spatial and temporal information of the GI samples [29]. The future use of these clinical techniques in oral biopharmaceutics expects to improve our understanding of the GI processes involved in oral drug delivery.…”

Section: Gastrointestinal Experimental Absorption Modelsmentioning

confidence: 99%

Intestinal Permeability and Drug Absorption: Predictive Experimental, Computational and In Vivo Approaches

2019

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The main objective of this review is to discuss recent advancements in the overall investigation and in vivo prediction of drug absorption. The intestinal permeability of an orally administered drug (given the value Peff) has been widely used to determine the rate and extent of the drug’s intestinal absorption (Fabs) in humans. Preclinical gastrointestinal (GI) absorption models are currently in demand for the pharmaceutical development of novel dosage forms and new drug products. However, there is a strong need to improve our understanding of the interplay between pharmaceutical, biopharmaceutical, biochemical, and physiological factors when predicting Fabs and bioavailability. Currently, our knowledge of GI secretion, GI motility, and regional intestinal permeability, in both healthy subjects and patients with GI diseases, is limited by the relative inaccessibility of some intestinal segments of the human GI tract. In particular, our understanding of the complex and highly dynamic physiology of the region from the mid-jejunum to the sigmoid colon could be significantly improved. One approach to the assessment of intestinal permeability is to use animal models that allow these intestinal regions to be investigated in detail and then to compare the results with those from simple human permeability models such as cell cultures. Investigation of intestinal drug permeation processes is a crucial biopharmaceutical step in the development of oral pharmaceutical products. The determination of the intestinal Peff for a specific drug is dependent on the technique, model, and conditions applied, and is influenced by multiple interactions between the drug molecule and the biological membranes.

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Section: Gastrointestinal Experimental Absorption Modelsmentioning

confidence: 99%

Intestinal Permeability and Drug Absorption: Predictive Experimental, Computational and In Vivo Approaches

2019

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“…Here, we describe the development and testing of an innovative noninvasive technology to sample the intestinal lumen in vivo. Beyond the prospects and opportunities for ingestible GI sampling devices discussed in a prior article, the proposed device is specifically targeted to study the gut microbiome. We created an ingestible, biocompatible, 3D‐printed microengineered pill with an integrated osmotic sampler that requires no battery for its operation.…”

Section: Introductionmentioning

confidence: 99%

Ingestible Osmotic Pill for In Vivo Sampling of Gut Microbiomes

Nejad

Oliviera

Sadeqi

et al. 2019

Advanced Intelligent Systems

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Technologies capable of noninvasively sampling different locations in the gut upstream of the colon enable new insights into the role of organ-specific microbiota in human health. Herein, an ingestible, biocompatible, battery-less, 3D-printed microengineered pill with an integrated osmotic sampler and microfluidic channels for in vivo sampling of the gut lumen and its microbiome upstream of the colon is discussed. The pill's sampling performance is characterized using realistic in vitro models and validated in vivo in pigs and primates. Herein, the results show that the bacterial populations recovered from the pill's microfluidic channels closely resemble the bacterial population demographics of the microenvironment to which the pill is exposed. Herein, it is believed that such lab-on-a-pill devices revolutionize the understanding of the spatial diversity of the gut microbiome and its response to medical conditions and treatments.

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“…[12][13][14] As an alternative approach, smart functional capsules with the ability to collect samples at different targeted locations in the entire GI tract can address several limitations associated with conventional colonoscopy and gastroscopy. 15,16 Furthermore, capsule-based devices can improve patient comfort, without the requirement of being administered in clinical settings. Perhaps one of the best-known smart capsules used for diagnostic and treatment in the eld of gastrointestinal disorder is the PillCam™ capsule endoscopy (CE) technology.…”

Section: Introductionmentioning

confidence: 99%

“…In active devices, the actuation and sampling mechanism are oen attained by using an on-board battery that provides the required energy to actuate various plungers, pistons, and biopsy forceps. 15,[19][20][21] For instance, Park et al designed an active capsule that takes tissue biopsies with a microactuator that moves forward and backward via slider-crank mechanism. 20 The triggering process of microactuator is achieved by electric power applied to the shape memory alloy heating wire.…”

Section: Introductionmentioning

confidence: 99%

“…23 To prevent such challenges, various passive actuation sampling mechanism approaches have been exploited by enabling the capsules to be more compact, economically viable, with fewer safetyassociated issues. 15 In such designs, the capsule moves through the GI tract via peristalsis motion with an average speed of 1-2 cm min À1 and the samples are collected through simple passive actuations such as capillary wicking actions and pressure differentials forces. 12 In a patent application, a capsule was designed with a vacuum interior and a sealed opening.…”

Section: Introductionmentioning

confidence: 99%

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Smart capsule for non-invasive sampling and studying of the gastrointestinal microbiome

et al. 2020

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Gut microbiota plays an important role in host physiology such as obesity, diabetes, and various neurological diseases. Thus, microbiome sampling is a fundamental approach towards better understanding of possible diseases. However, conventional sampling methods, such as endoscopies or colonoscopies, are invasive and cannot reach the entire small intestine. To address this need, a batteryless 3D-printed sampling capsule, which can collect microbiome samples throughout the entirety of the GI tract was designed. The capsule (9 mm Â 15 mm) consists of a 3D printed acrylic housing, a fastabsorbing hydrogel, and a flexible PDMS membrane. Fluids containing samples of the microbial flora within the GI tract enter the device through a sampling aperture on the cap of the device. Once the microbiome enters the housing, the hydrogel absorbs the fluid and swells, effectively protecting the samples within its polymeric matrix, while also pushing on the flexible PDMS membrane to block the sampling aperture from further fluid exchange. The retrieved capsule can be readily disassembled due to the screw-cap design of the capsule and the hydrogel can be removed for further bacterial culture and analysis. As a proof of concept, the capsule's bacterial sampling efficiency and the ability to host microbial samples within the hydrogel in a sealed capsule were validated using a liquid culture containing Escherichia coli. The demonstrated technology provides a promising inexpensive tool for direct sampling and assessment of microbes throughout the GI tract and can enable new insights into the role of diet in mediating host-microbe interactions and metabolism.

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Ingestible Gastrointestinal Sampling Devices: State-of-the-Art and Future Directions

Cited by 22 publications

References 99 publications

Intestinal Permeability and Drug Absorption: Predictive Experimental, Computational and In Vivo Approaches

Intestinal Permeability and Drug Absorption: Predictive Experimental, Computational and In Vivo Approaches

Ingestible Osmotic Pill for In Vivo Sampling of Gut Microbiomes

Smart capsule for non-invasive sampling and studying of the gastrointestinal microbiome

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