Infrequent patterns of upper gastrointestinal tract malignancy

Abstract

The skin and the upper gastrointestinal tract are frequent sites of malignant tumors. However, cutaneous metastasis from primary gastrointestinal tract lesions remains a relative rarity. Similarly rare is spread of primary skin cancer (nonmelanomatous) to upper gastrointestinal tract. This paper presents an unusual example of primary esophageal carcinoma with synchronous skin and bone metastases and a case of squamous cell carcinoma of the stomach, possibly due to spread from a remote squamous cell carcinoma of the skin.

A chromatographic and immunoprofiling approach to optimising workflows for extraction of gluten proteins from flour

“…Parameters for the transfer were 15 V, 300 A, 100 W for 35 mins for two gels. Any free binding sites on the nitrocellulose membrane were blocked by incubation with phosphate buffered saline (137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.4, 0.05 % (v/v) Tween-20 and 5 % (w/ v) skimmed milk powder (blocking buffer) overnight at 4 • C. After washing, membranes were incubated for 1 h at ambient temperature with primary anti-gluten antibodies with the following dilutions (v:v) in blocking buffer: R5 − 1:100, G12 -1:1000, IFRN0610 -1:100 and ONT18A5 -1:500 [38]. After washing, membranes were incubated with secondary goat anti-mouse IgG antibody conjugated to AP (diluted 1:5000, v:v in blocking buffer) for 1 h at ambient temperature.…”